Improving plant reproductive success under heat stress: A sweet approach. This project aims to determine how genetic manipulation of cell wall invertase (CWIN) activity could regulate pollen germination, elongation and fruit set under heat stress using tomato as a model. Plant reproductive processes are highly susceptible to heat stress, which often leads to pollination failure and fruit and seed abortion, hence irreversible yield loss. Research has established that CWIN-mediated sugar metabolis ....Improving plant reproductive success under heat stress: A sweet approach. This project aims to determine how genetic manipulation of cell wall invertase (CWIN) activity could regulate pollen germination, elongation and fruit set under heat stress using tomato as a model. Plant reproductive processes are highly susceptible to heat stress, which often leads to pollination failure and fruit and seed abortion, hence irreversible yield loss. Research has established that CWIN-mediated sugar metabolism and signaling may play crucial roles in pollen growth and fruit set under heat stress. The intended outcome is the generation of critical knowledge that will advance understanding on reproductive development under heat stress, thereby providing significant benefits, such as novel ideas and solutions for improving crop yield.Read moreRead less
The use of molecular sponges to inhibit small Ribonucleic acid activity in plants. The deletion of gene activity is the most powerful way to understand gene function; however for genes encoding small Ribonucleic acids (RNAs) no current methodology can efficiently achieve this. Here, we aim to develop a gene silencing technology for small RNA encoding genes, which can be utilised to determine their function and used for biotechnological applications.
Can altered sugar sensing improve crop productivity? This project aims at genetically manipulating sugar sensing pathways in the model C4 grass Setaria viridis, and at replacing sugar sensors in the model C3 crop Oryza sativa (rice) with those from S. viridis. This project expects to elucidate the impact of altered sugar perception on crop photosynthesis and yield. Expected outcomes includes advancing a novel “pull” approach to improve yield in C3 crops by using C4-like sugar sensors to reduce f ....Can altered sugar sensing improve crop productivity? This project aims at genetically manipulating sugar sensing pathways in the model C4 grass Setaria viridis, and at replacing sugar sensors in the model C3 crop Oryza sativa (rice) with those from S. viridis. This project expects to elucidate the impact of altered sugar perception on crop photosynthesis and yield. Expected outcomes includes advancing a novel “pull” approach to improve yield in C3 crops by using C4-like sugar sensors to reduce feedback regulation of photosynthesis which in turn limits productivity. This is in contrast to previous ‘push’ approaches aimed at directly increasing photosynthesis. Hence, this project provides significant benefits by contributing to the next green revolution needed to lift agricultural yields.Read moreRead less
The hunt for Ribonucleic Acid riboswitches and genetic sensors of metabolic flux in plants. Ribonucleic Acid (RNA) contains both structural and sequence information that coordinates feedback of metabolic processes in response to environmental change, thereby promoting cellular adaptation and survival. This project will discover ancient RNA modules and structural switches in plants that sense chemical reactions and regulate pathway flux.
Boosting C4 photosynthesis to climate proof crop yields. Building next generation C4 crops, such as maize, sugarcane and sorghum, to cope with drought and heat stress is requisite to ensure the supply of food and fodder. Here we will increase the content and / or catalytic efficiency of the primary carboxylase of C4 photosynthesis (PEPC) that supplies CO2 to the carbon concentrating mechanism and ensures high photosynthetic rates. We will develop new SynBio tools to create and test novel PEPC is ....Boosting C4 photosynthesis to climate proof crop yields. Building next generation C4 crops, such as maize, sugarcane and sorghum, to cope with drought and heat stress is requisite to ensure the supply of food and fodder. Here we will increase the content and / or catalytic efficiency of the primary carboxylase of C4 photosynthesis (PEPC) that supplies CO2 to the carbon concentrating mechanism and ensures high photosynthetic rates. We will develop new SynBio tools to create and test novel PEPC isoforms with desirable properties. Ultimately, the project aims to identify isoforms that improve plant fitness under stress conditions. Optimising PEPC activity will provide next generation solutions to improve water balance and carbon assimilation to keep C4 crops productive under future climates.Read moreRead less
Oxygen isotope discrimination during C4 photosynthesis. Plants with the C4 photosynthetic pathway, like sugarcane and pasture grasses, are vital to Australian agriculture and natural ecosystems. This project will use novel laser spectroscopy to measure oxygen isotope discrimination during photosynthesis and quantify the influence of C4 plants on isotopic signatures of atmospheric CO2.
Leaves in 3D: photosynthesis and water-use efficiency. This project aims to develop leaf anatomical ideotypes with improved photosynthesis and water-use efficiency for wheat, rice, chickpea and cotton using novel three dimensional imaging and modelling techniques. This project expects to generate new understanding of the role of leaf anatomy on leaf function. Expected outcomes of this project include the world's first 3D spatially-explicit, anatomically accurate model of leaves of crop plants to ....Leaves in 3D: photosynthesis and water-use efficiency. This project aims to develop leaf anatomical ideotypes with improved photosynthesis and water-use efficiency for wheat, rice, chickpea and cotton using novel three dimensional imaging and modelling techniques. This project expects to generate new understanding of the role of leaf anatomy on leaf function. Expected outcomes of this project include the world's first 3D spatially-explicit, anatomically accurate model of leaves of crop plants to allow virtual experiments identifying optimized anatomy for improved photosynthetic performance. Benefits to the agricultural industry include increased crop productivity and water-use efficiency to meet future global food demand and to make the most of Australia's limited water resourcesRead moreRead less
Harnessing peptide hormone outputs to improve root nodulation’s efficacy. This project aims to transform our understanding of symbiotic nitrogen fixation in legume root nodules. Root nodulation sustainably fixes sizeable amounts of nitrogen to boost crop production worldwide yet its utilisation is waning in favour of using nitrogen fertilisers. The project applies cutting-edge tools to define how two hormone systems boost and limit nitrogen fixation, respectively. The project expects to reveal w ....Harnessing peptide hormone outputs to improve root nodulation’s efficacy. This project aims to transform our understanding of symbiotic nitrogen fixation in legume root nodules. Root nodulation sustainably fixes sizeable amounts of nitrogen to boost crop production worldwide yet its utilisation is waning in favour of using nitrogen fertilisers. The project applies cutting-edge tools to define how two hormone systems boost and limit nitrogen fixation, respectively. The project expects to reveal ways to reconfigure these hormone outputs to improve nodule number and the efficacy of nitrogen fixation. The findings will benefit agriculture by reducing the reliance on costly nitrogen fertilisers, thus mitigating the huge environmental damage they cause, and will provide more sustainable ways to ensure food security.Read moreRead less
Remodelling encapsulin nanocages to help enhance plant carbon fixation. Nature has evolved mechanisms in microbial systems to improve photosynthetic efficiency by saturating the enzyme Rubisco with carbon dioxide. These carbon concentrating mechanisms are genetically complex, precluding successful introduction into crops. Our simpler approach is to use encapsulins, a new source of robust bacterial pore-containing nanocages made from a single gene. This project will optimise the development of sy ....Remodelling encapsulin nanocages to help enhance plant carbon fixation. Nature has evolved mechanisms in microbial systems to improve photosynthetic efficiency by saturating the enzyme Rubisco with carbon dioxide. These carbon concentrating mechanisms are genetically complex, precluding successful introduction into crops. Our simpler approach is to use encapsulins, a new source of robust bacterial pore-containing nanocages made from a single gene. This project will optimise the development of synthetic encapsulin-Rubisco carbon-fixing nanoreactors and transform them into leaf chloroplasts to test their impact on plant photosynthesis and growth. Our genetically simpler solution will aid ongoing global efforts to deliver overdue step change improvements in agricultural productivity.Read moreRead less
Salinity tolerance along an aridity gradient: linking physiological processes with morphological constraints on leaf function in mangroves. The proposed research will provide insight into the physiological and morphological features that control the productivity of mangrove forests across broad gradients in salinity and aridity. Central to this is this identification of plant traits that increase salt and drought tolerance, which will assist in the development of plant varieties suited to Austra ....Salinity tolerance along an aridity gradient: linking physiological processes with morphological constraints on leaf function in mangroves. The proposed research will provide insight into the physiological and morphological features that control the productivity of mangrove forests across broad gradients in salinity and aridity. Central to this is this identification of plant traits that increase salt and drought tolerance, which will assist in the development of plant varieties suited to Australian conditions. The results will also contribute to development of process-based models to better predict the response of mangrove vegetation to changing climate. A deep understanding of the processes that influence the growth and survival of mangroves is of fundamental importance to sustainable fisheries and protection of wildlife reliant on coastal ecosystems. Read moreRead less