Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0668294
Funder
Australian Research Council
Funding Amount
$110,000.00
Summary
Isotope Ratio Mass Spectrometry Facility for Nitrogen and Water Analysis in Plants. Continual improvement to agricultural plant production is key to maintaining future sustainable growth in Australian agriculture. Our respective research teams are focussed on improving how plants utilise both nitrogen and water. Many questions remain with respect to where, how and when plants use and or access these important nutrients. The proposed facility will enable plant scientists to begin in-depth anal ....Isotope Ratio Mass Spectrometry Facility for Nitrogen and Water Analysis in Plants. Continual improvement to agricultural plant production is key to maintaining future sustainable growth in Australian agriculture. Our respective research teams are focussed on improving how plants utilise both nitrogen and water. Many questions remain with respect to where, how and when plants use and or access these important nutrients. The proposed facility will enable plant scientists to begin in-depth analysis of both nitrogen transport mechanisms and the ability to model root development and water allocation in crop species. This research will ultimately lead to improved knowledge on how plants respond to their environment and where modifications can be made to generate sustainable crops suited to Australian agriculture.Read moreRead less
IMPROVING NITROGEN USE EFFICIENCY IN CROP PLANTS: ROLE OF THE AMMONIUM TRANSPORT FAMILY AMT. Improving nitrogen use efficiency in crop plants will reduce the use of environmentally damaging nitrogen fertilisers that threaten through leaching the sustainability of Australia's agricultural sector and local water ecosystems. Plants contain genes that encode transport proteins required for the uptake of nitrogen (ammonium and nitrate) from the soil. We will identify the in planta activity of the A ....IMPROVING NITROGEN USE EFFICIENCY IN CROP PLANTS: ROLE OF THE AMMONIUM TRANSPORT FAMILY AMT. Improving nitrogen use efficiency in crop plants will reduce the use of environmentally damaging nitrogen fertilisers that threaten through leaching the sustainability of Australia's agricultural sector and local water ecosystems. Plants contain genes that encode transport proteins required for the uptake of nitrogen (ammonium and nitrate) from the soil. We will identify the in planta activity of the AMT family of ammonium transporters and associated signalling pathways which control the uptake and assimilation of ammonium in plants. This project will confirm the mechanisms involved in ammonium uptake from the soil and lead to the development of ammonium-nitrogen efficient crop plants.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0561161
Funder
Australian Research Council
Funding Amount
$110,000.00
Summary
Joint Facility for Genome Analysis of Nutrient Transport Proteins. The joint facility for genome analysis of nutrient transport proteins is a new initiative between the University of Adelaide, the Australian Centre for Plant Functional Genomics, and the University of Western Australia to use a high throughput Xenopus oocyte expression system to screen plant cDNA/cRNA collections for genes encoding nutrient transport proteins. The facility will also provide a platform to rapidly accelerate our p ....Joint Facility for Genome Analysis of Nutrient Transport Proteins. The joint facility for genome analysis of nutrient transport proteins is a new initiative between the University of Adelaide, the Australian Centre for Plant Functional Genomics, and the University of Western Australia to use a high throughput Xenopus oocyte expression system to screen plant cDNA/cRNA collections for genes encoding nutrient transport proteins. The facility will also provide a platform to rapidly accelerate our present capacity for Xenopus oocyte expression analysis of nutrient transport proteins. This facility will greatly aid our current research quantum in this field and allow for new discoveries related to nutrient transport in plants.Read moreRead less
Salinity tolerance and long-distance transport in cereals. The aim of this program is to alter shoot accumulation of solutes in cereals by exploiting novel transgenic technology to manipulate processes in specific cell types in the roots. The primary objective is the generation of cereals which have increased tolerance of saline soils. This is clearly of much agricultural significance in Australia. More general outcomes include the generation of plants with altered concentrations of a range of n ....Salinity tolerance and long-distance transport in cereals. The aim of this program is to alter shoot accumulation of solutes in cereals by exploiting novel transgenic technology to manipulate processes in specific cell types in the roots. The primary objective is the generation of cereals which have increased tolerance of saline soils. This is clearly of much agricultural significance in Australia. More general outcomes include the generation of plants with altered concentrations of a range of nutrients in both leaves and grain. This will be of wide agricultural and nutritional benefit, as well as providing an understanding of principles underlying the long-distance co-ordination of processes in plants.Read moreRead less
Aquaporins in roots: resolving observations linking them to diverse processes in water relations and plant productivity. The knowledge we gain will benefit Australia by allowing better management of plant water use and productivity. This is critical for adaptation to a drier climate where water is a critical resource. Large quantities of water move through aquaporin proteins in plants, therefore our understanding of these and the way they influence other processes in plant growth could enable us ....Aquaporins in roots: resolving observations linking them to diverse processes in water relations and plant productivity. The knowledge we gain will benefit Australia by allowing better management of plant water use and productivity. This is critical for adaptation to a drier climate where water is a critical resource. Large quantities of water move through aquaporin proteins in plants, therefore our understanding of these and the way they influence other processes in plant growth could enable us to manipulate plants to conserve water or to extract it more efficiently from the soil. Molecular aspects of the project could reveal new unexploited links between water and plant productivity. High calibre PhD and Honours students will also be educated to maintain the momentum of international excellence within Australia in the field of plant water relations.Read moreRead less
Control of foliar diseases in horticulture using milk components: widening applicability through understanding mechanisms. Fungal diseases, such as powdery mildew and botrytis grey mould, have the potential to cause considerable losses in horticultural crops. Chemical fungicides, some of which are broad-spectrum biocides potentially harmful to human health, are applied routinely in disease management. Milk and whey, which can damage powdery mildew fungi, offer alternatives to conventional fungic ....Control of foliar diseases in horticulture using milk components: widening applicability through understanding mechanisms. Fungal diseases, such as powdery mildew and botrytis grey mould, have the potential to cause considerable losses in horticultural crops. Chemical fungicides, some of which are broad-spectrum biocides potentially harmful to human health, are applied routinely in disease management. Milk and whey, which can damage powdery mildew fungi, offer alternatives to conventional fungicides. Identification of the components of milk which damage fungi, and their mechanisms of activity, will facilitate the development of environmentally sustainable strategies for management of fungal diseases in Australian horticulture. This will have particular benefits for personnel who regularly apply fungicides in glasshouses.Read moreRead less
Improving nitrogen use efficiency in crop plants: Functional analysis of high-affinity ammonium transport in plant lines altered in ammonium transport capacity. Improving nitrogen fertiliser use in crop species will benefit Australia and world agriculture by reducing nitrogen-linked environmental pollution. Nitrogen fertilisers can be leached from soils and result in the pollution of ground water, rivers, estuaries and oceans. Using model plant systems we are dissecting the genes and their encod ....Improving nitrogen use efficiency in crop plants: Functional analysis of high-affinity ammonium transport in plant lines altered in ammonium transport capacity. Improving nitrogen fertiliser use in crop species will benefit Australia and world agriculture by reducing nitrogen-linked environmental pollution. Nitrogen fertilisers can be leached from soils and result in the pollution of ground water, rivers, estuaries and oceans. Using model plant systems we are dissecting the genes and their encoded proteins responsible for ammonium nitrogen uptake into plants. Identifying the in planta function of these genes will allow new breeding approaches to specifically target genes that will improve the efficiency of ammonium uptake and lessen the reliance on the supply of nitrogen fertilizers in modern agricultural crop production.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE150100837
Funder
Australian Research Council
Funding Amount
$354,000.00
Summary
Engineering enzymes controlling plant polysaccharide properties. This project will aim to use data to define how the synthesis and interconversion of nucleotide sugars is regulated and how this controls the properties of arabinoxylan in economically important plants. Dietary consumption of arabinoxylan reduces chronic diseases. Additionally, the attributes of arabinoxylan influence the cost of processing plant biomass. However, genetic control of the properties of the plant polysaccharide arabin ....Engineering enzymes controlling plant polysaccharide properties. This project will aim to use data to define how the synthesis and interconversion of nucleotide sugars is regulated and how this controls the properties of arabinoxylan in economically important plants. Dietary consumption of arabinoxylan reduces chronic diseases. Additionally, the attributes of arabinoxylan influence the cost of processing plant biomass. However, genetic control of the properties of the plant polysaccharide arabinoxylan is unresolved. A major control point in the partitioning of carbon from photosynthesis into arabinoxylan is the activity of sugar nucleotide interconverting enzymes. To characterise these enzymes, genomic, glycomic and enzyme kinetic data will be combined and the target enzymes will be modified in transgenic plants.Read moreRead less
Symbiotic transport proteins in legumes. Some plants form a symbiosis with soil bacteria (rhizobia) that convert atmospheric nitrogen to ammonia which is then supplied to the plant. This enables legumes to grow without application of nitrogen-based fertilizer, avoiding environmental problems such as run-off and land degradation, thereby contributing to sustainable agriculture practise. We will investigate the interactions between plant and rhizobia, focusing on identifying genes and proteins wh ....Symbiotic transport proteins in legumes. Some plants form a symbiosis with soil bacteria (rhizobia) that convert atmospheric nitrogen to ammonia which is then supplied to the plant. This enables legumes to grow without application of nitrogen-based fertilizer, avoiding environmental problems such as run-off and land degradation, thereby contributing to sustainable agriculture practise. We will investigate the interactions between plant and rhizobia, focusing on identifying genes and proteins which govern nutrient exchange between the partners and development of the special structures in the roots that house the bacteria. Subsequent manipulation of these genes and proteins may allow us to identify control points and enhance nitrogen fixation.
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Molecular analysis of the symbiotic interface of nitrogen-fixing legumes. Some legumes form a symbiosis with soil bacteria (rhizobia) that convert atmospheric nitrogen to ammonia which is then supplied to the plant. This enables legumes to grow without application of nitrogen-based fertilizer, avoiding environmental problems such as run-off and land degradation, thereby contributing to sustainable agriculture practise. We will investigate the interactions between plant and rhizobia, focusing on ....Molecular analysis of the symbiotic interface of nitrogen-fixing legumes. Some legumes form a symbiosis with soil bacteria (rhizobia) that convert atmospheric nitrogen to ammonia which is then supplied to the plant. This enables legumes to grow without application of nitrogen-based fertilizer, avoiding environmental problems such as run-off and land degradation, thereby contributing to sustainable agriculture practise. We will investigate the interactions between plant and rhizobia, focusing on identifying genes and proteins which govern nutrient exchange between the partners and development of the special structures in the roots that house the bacteria. Subsequent manipulation of these genes and proteins may allow us to identify control points and enhance nitrogen fixation.Read moreRead less