Practical application of gene silencing: is delivery of long double stranded ribonucleic acid (dsRNA) by plant cells efficient in conferring host resistance to parasitic nematodes? Nematode that attack plants cause $120 billion of crop losses worldwide. Chemicals used for their control are being phased out because of environmental concerns, and natural resistance is limited. The aim of this project is to use Australian IP to develop a new form of resistance to nematodes based on knowledge of th ....Practical application of gene silencing: is delivery of long double stranded ribonucleic acid (dsRNA) by plant cells efficient in conferring host resistance to parasitic nematodes? Nematode that attack plants cause $120 billion of crop losses worldwide. Chemicals used for their control are being phased out because of environmental concerns, and natural resistance is limited. The aim of this project is to use Australian IP to develop a new form of resistance to nematodes based on knowledge of the host-pathogen interactions. A successful outcome could contribute an additional 5-20% increase in crop yields (depending on the crop) through inherent resistance of crops to nematode pests. This would benefit rural communities and the national economy, and could also generate international royalties.Read moreRead less
The response of mitochondria to oxidative stress in plants. Crops encounter many situations in their environment which place them under stress. Reactive oxygen molecules produced in these situations act as messengers to trigger defence mechanisms but also cause cellular damage. Mitochondria are the subcellular compartments involved in energy production and are essential for plant development and growth. However, they also have been implicated in the response of plants to stress and pathogen atta ....The response of mitochondria to oxidative stress in plants. Crops encounter many situations in their environment which place them under stress. Reactive oxygen molecules produced in these situations act as messengers to trigger defence mechanisms but also cause cellular damage. Mitochondria are the subcellular compartments involved in energy production and are essential for plant development and growth. However, they also have been implicated in the response of plants to stress and pathogen attack, and in production of reactive oxygen molecules. This proposal seeks to investigate how mitochondria are involved in these processes, focusing on the role of terminal oxidases. Potential outcomes include crops better able to cope with environmental stress.Read moreRead less
Finding damage thresholds in pyrethrum to optimise crop profitability. This project aims to use a new vascular approach to develop a quantitative stress tolerance framework for the crop species pyrethrum, defining the risks to plant production of water, heat and frost stress. Using novel optical and x-ray technology, this project seeks to pinpoint damaging stress thresholds and combine this knowledge with crop monitoring technology in a way that will allow crop managers to avoid damaging stress ....Finding damage thresholds in pyrethrum to optimise crop profitability. This project aims to use a new vascular approach to develop a quantitative stress tolerance framework for the crop species pyrethrum, defining the risks to plant production of water, heat and frost stress. Using novel optical and x-ray technology, this project seeks to pinpoint damaging stress thresholds and combine this knowledge with crop monitoring technology in a way that will allow crop managers to avoid damaging stress events. The intended outcome is to enable the pyrethrum industry, and ultimately a diversity of crop managers, to better utilise new advances in monitoring technology to maximise the benefits of irrigation such that yields are high relative to water use and damage by stress is avoided. Immediate beneficiaries will be the pyrethrum industry, but the research will provide a model, applicable to the multitude of irrigated crops in Australia. Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0775702
Funder
Australian Research Council
Funding Amount
$337,000.00
Summary
Climate controlled physical containment 2 (PC2) and pathogen/ insect contained glasshouse facility. The Australian economy relies heavily on agricultural production. The outcomes of the projects supported by these growth facilities will be of economic benefit to the nation by producing new knowledge of plant-insect and plant-pathogen interactions, how plants acquire essential nutrients, and how they respond to environmental stress. The research outcomes will benefit the environment by increasin ....Climate controlled physical containment 2 (PC2) and pathogen/ insect contained glasshouse facility. The Australian economy relies heavily on agricultural production. The outcomes of the projects supported by these growth facilities will be of economic benefit to the nation by producing new knowledge of plant-insect and plant-pathogen interactions, how plants acquire essential nutrients, and how they respond to environmental stress. The research outcomes will benefit the environment by increasing legume production and so reducing land degradation and risk of nitrate contamination of waterways and lowering the environmental risk from agrochemicals by developing safer strategies for control of pests and diseases. In addition, a number of projects that will benefit from the glasshouse facilities aim to produce healthier and safer foods.Read moreRead less
Improving plant reproductive success under heat stress: A sweet approach. This project aims to determine how genetic manipulation of cell wall invertase (CWIN) activity could regulate pollen germination, elongation and fruit set under heat stress using tomato as a model. Plant reproductive processes are highly susceptible to heat stress, which often leads to pollination failure and fruit and seed abortion, hence irreversible yield loss. Research has established that CWIN-mediated sugar metabolis ....Improving plant reproductive success under heat stress: A sweet approach. This project aims to determine how genetic manipulation of cell wall invertase (CWIN) activity could regulate pollen germination, elongation and fruit set under heat stress using tomato as a model. Plant reproductive processes are highly susceptible to heat stress, which often leads to pollination failure and fruit and seed abortion, hence irreversible yield loss. Research has established that CWIN-mediated sugar metabolism and signaling may play crucial roles in pollen growth and fruit set under heat stress. The intended outcome is the generation of critical knowledge that will advance understanding on reproductive development under heat stress, thereby providing significant benefits, such as novel ideas and solutions for improving crop yield.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0561161
Funder
Australian Research Council
Funding Amount
$110,000.00
Summary
Joint Facility for Genome Analysis of Nutrient Transport Proteins. The joint facility for genome analysis of nutrient transport proteins is a new initiative between the University of Adelaide, the Australian Centre for Plant Functional Genomics, and the University of Western Australia to use a high throughput Xenopus oocyte expression system to screen plant cDNA/cRNA collections for genes encoding nutrient transport proteins. The facility will also provide a platform to rapidly accelerate our p ....Joint Facility for Genome Analysis of Nutrient Transport Proteins. The joint facility for genome analysis of nutrient transport proteins is a new initiative between the University of Adelaide, the Australian Centre for Plant Functional Genomics, and the University of Western Australia to use a high throughput Xenopus oocyte expression system to screen plant cDNA/cRNA collections for genes encoding nutrient transport proteins. The facility will also provide a platform to rapidly accelerate our present capacity for Xenopus oocyte expression analysis of nutrient transport proteins. This facility will greatly aid our current research quantum in this field and allow for new discoveries related to nutrient transport in plants.Read moreRead less
Coal-ash as a resource for sustainable soil-management in plant production systems. Acidification and salinisation are major causes of land degradation in Australia, costing $1-2 billion to combat. Farmers used 1.9M t of lime and 1.0M t of gypsum to manage their soils in 2000. Power stations annually produce 12M t of coal-ash that have functional characteristics of lime and gypsum and can potentially ameliorate degraded land. Presently only 43% of the ash is used and almost entirely for constr ....Coal-ash as a resource for sustainable soil-management in plant production systems. Acidification and salinisation are major causes of land degradation in Australia, costing $1-2 billion to combat. Farmers used 1.9M t of lime and 1.0M t of gypsum to manage their soils in 2000. Power stations annually produce 12M t of coal-ash that have functional characteristics of lime and gypsum and can potentially ameliorate degraded land. Presently only 43% of the ash is used and almost entirely for construction with the rest going into landfills. This project will determine the sustainable use of coal-ash to improve structure and nutritive properties of the soil and to raise crop yield across several sites in Australia.Read moreRead less
Regulation of Plant Development by Small RNAs. Understanding the roles of small RNAs and their pathways is a new field of research that is giving, and will continue to give profound insights into how multicellular organisms regulate gene expression at a genomic level. Research in this area has already led to RNA interference technology, by which almost any gene can be switched off, and there is considerable potential for other gene silencing and trait modification technologies to emerge. The pro ....Regulation of Plant Development by Small RNAs. Understanding the roles of small RNAs and their pathways is a new field of research that is giving, and will continue to give profound insights into how multicellular organisms regulate gene expression at a genomic level. Research in this area has already led to RNA interference technology, by which almost any gene can be switched off, and there is considerable potential for other gene silencing and trait modification technologies to emerge. The project will yield insights into fundamental biological processes which are expected to engender applications in agriculture and biotechnology. It will maintain and enhance Australia's position in this area.Read moreRead less
Small RNAs: what makes a plant, a plant. Understanding the roles of small RNAs and their pathways is a young field of research that is giving, and will continue to give, profound insights into how multicellular organisms regulate gene expression at a genomic level. Research in this area has already led to RNA interference technology, by which almost any gene can be switched off, and there is considerable potential for other gene silencing and trait modification technologies to emerge. The projec ....Small RNAs: what makes a plant, a plant. Understanding the roles of small RNAs and their pathways is a young field of research that is giving, and will continue to give, profound insights into how multicellular organisms regulate gene expression at a genomic level. Research in this area has already led to RNA interference technology, by which almost any gene can be switched off, and there is considerable potential for other gene silencing and trait modification technologies to emerge. The project will yield insights into fundamental biological processes which are expected to engender applications in agriculture and biotechnology. It will maintain and enhance Australia's position in this area.Read moreRead less
The use of molecular sponges to inhibit small Ribonucleic acid activity in plants. The deletion of gene activity is the most powerful way to understand gene function; however for genes encoding small Ribonucleic acids (RNAs) no current methodology can efficiently achieve this. Here, we aim to develop a gene silencing technology for small RNA encoding genes, which can be utilised to determine their function and used for biotechnological applications.