Why is the peribacteroid membrane transcription factor SAT1 required for legume nitrogen fixation and what is its role in other symbiotic systems? This project will investigate the functional activity of the plant membrane bound basic helix-loop-helix (bHLH) transcription factor SAT1 in both nitrogen fixing (Rhizobia) and phosphorus acquiring (Arbuscular Mycorrhizal) symbioses found in plants. The project will identify its regulation and downstream activities across both symbiosis using selected ....Why is the peribacteroid membrane transcription factor SAT1 required for legume nitrogen fixation and what is its role in other symbiotic systems? This project will investigate the functional activity of the plant membrane bound basic helix-loop-helix (bHLH) transcription factor SAT1 in both nitrogen fixing (Rhizobia) and phosphorus acquiring (Arbuscular Mycorrhizal) symbioses found in plants. The project will identify its regulation and downstream activities across both symbiosis using selected legumes and or cereals.Read moreRead less
Nutrient transfer across symbiotic membranes in soybean. Legume plants interact with soil bacteria that fix nitrogen from the air to obtain nitrogen required for growth, reducing their use of fertilisers. Understanding how nutrients are exchanged between bacteria and legumes may improve the efficiency of this process. This would make legumes a more valuable component of sustainable agricultural systems.
Molecular Resolution 3D Atlas of the Photosynthetic Machinery. The project aims to produce an atomic-resolution 3-D atlas of the photosynthetic machinery of single-cell green algae to guide the targeted engineering of high efficiency algae production cell lines and bio-inspired artificial solar fuel systems. Photosynthesis drives the first step of all algae production processes by capturing solar energy and converting it to chemical energy (for example sustainable fuels, food and high value prod ....Molecular Resolution 3D Atlas of the Photosynthetic Machinery. The project aims to produce an atomic-resolution 3-D atlas of the photosynthetic machinery of single-cell green algae to guide the targeted engineering of high efficiency algae production cell lines and bio-inspired artificial solar fuel systems. Photosynthesis drives the first step of all algae production processes by capturing solar energy and converting it to chemical energy (for example sustainable fuels, food and high value products), but excess light can cause photodamage. Microalgae have evolved intricate photo-protection mechanisms that can dissipate up to 90 per cent of the captured light energy. Fine-tuning the light harvesting complexes could considerably increase efficiency.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE100100078
Funder
Australian Research Council
Funding Amount
$600,000.00
Summary
Multiphoton confocal microscope. Recent developments in light microscopy have revolutionised modern molecular and cellular biology. Dramatic improvements in microscope hardware and software and in the range of fluorescent markers used to tag selected cellular components now provide new and exciting opportunities to localise and determine the function of ions and molecules not only in preserved samples but also, most excitingly, in living cells. The proposed multiphoton confocal microscope will ....Multiphoton confocal microscope. Recent developments in light microscopy have revolutionised modern molecular and cellular biology. Dramatic improvements in microscope hardware and software and in the range of fluorescent markers used to tag selected cellular components now provide new and exciting opportunities to localise and determine the function of ions and molecules not only in preserved samples but also, most excitingly, in living cells. The proposed multiphoton confocal microscope will allow researchers in Canberra to obtain high quality images of static and moving components in living cells and tissues and will facilitate the discovery of new knowledge that contributes to our understanding and control of development and disease in both plants and animals.Read moreRead less
Does plasma membrane perception of 2,4-D influence auxin resistance? This project aims to investigate the role of the cell membrane in synthetic auxin herbicide resistance by analysing the functions and interaction partners of candidate resistance proteins. It is expected that this project will generate new knowledge about the very early response of plants to auxin and the difference between susceptible and resistant weeds in perceiving auxin herbicides. Expected outcomes of this project include ....Does plasma membrane perception of 2,4-D influence auxin resistance? This project aims to investigate the role of the cell membrane in synthetic auxin herbicide resistance by analysing the functions and interaction partners of candidate resistance proteins. It is expected that this project will generate new knowledge about the very early response of plants to auxin and the difference between susceptible and resistant weeds in perceiving auxin herbicides. Expected outcomes of this project include the identification of potential herbicide synergists and a greater understanding of how weeds develop resistance to auxin herbicides. This should benefit Australian grain growers by providing more effective weed control options and lessening the amount of unnecessarily-applied herbicide in the environment.Read moreRead less
Identification of the molecular targets on filamentous fungi that lead to specific recognition and killing by an antifungal plant defensin. Tobacco flowers naturally produce potent antifungal molecules for protection against disease. The purpose of this project is to understand why these molecules are so toxic to fungal pathogens with a view to using them for control of fungal diseases in crops and humans.
How do MACPF/CDC proteins punch giant holes in lipid membranes? This project aims to study the Membrane Attack Complex (MAC)/Perforin-like/Cholesterol Dependent Cytolysins (MACPF/CDC) family which form unusually large holes in membranes. This project aims to define the exact molecular shape of a monomer in comparison with the exact molecular shape of the pore of the MACPF/CDC family. This project will also provide new information about the intermediate steps in pore formation. This will have maj ....How do MACPF/CDC proteins punch giant holes in lipid membranes? This project aims to study the Membrane Attack Complex (MAC)/Perforin-like/Cholesterol Dependent Cytolysins (MACPF/CDC) family which form unusually large holes in membranes. This project aims to define the exact molecular shape of a monomer in comparison with the exact molecular shape of the pore of the MACPF/CDC family. This project will also provide new information about the intermediate steps in pore formation. This will have major benefits to agribusiness and nanotechnology applications.Read moreRead less
Phage display derived antibody fragments for membrane protein research. Membrane proteins are key components of all living organisms and represent more than 50 per cent of all drug targets. This project will redefine the way membrane proteins are studied and will be highly beneficial to basic research, human disease and the biotechnology industry.
Multiscale Analysis Of Plasma Membrane Microdomains In Health And Disease
Funder
National Health and Medical Research Council
Funding Amount
$863,413.00
Summary
The cell surface encloses the cell in a protective barrier but it must also respond to signals coming from outside the cell. To accomplish this, the cell surface is made up of numerous regions each with a specialised role. This proposal aims to examine how lipids and proteins work together to make these specialised regions and aims to understand what goes wrong in diseases such as muscular dystrophy.
The mechanism of pore formation by Membrane Attack Complex/Perforin-like proteins. Members of the Membrane Attack Complex / Perforin (MACPF) family of proteins are essential for life, playing fundamental roles in immunity, tissue development and neuron formation. This project seeks to understand the basic mechanism of how MACPF proteins can form pores in target cells, a process central for killing in mammalian immunity.