Phylogeny As A Basis For Molecular Identification Of Pathogenic Fungi
Funder
National Health and Medical Research Council
Funding Amount
$440,750.00
Summary
Pathogenic fungi are becoming increasingly important in causing potentially life-threatening diseases in immunocompromised hosts (e.g. AIDS, transplant patients). Many of the emerging fungal pathogens are inherently resistent to triazole antifungal drugs and clinical responses to established drugs remain suboptimal, despite apparent sensitivity in the laboratory. Current techniques of fungal identification are insensitive, unspecific, slow, labour-intensive and require skilled personnel for the ....Pathogenic fungi are becoming increasingly important in causing potentially life-threatening diseases in immunocompromised hosts (e.g. AIDS, transplant patients). Many of the emerging fungal pathogens are inherently resistent to triazole antifungal drugs and clinical responses to established drugs remain suboptimal, despite apparent sensitivity in the laboratory. Current techniques of fungal identification are insensitive, unspecific, slow, labour-intensive and require skilled personnel for the ID of less common fungi. To improve clinical outcomes by prompt selection-initiation of the best antifungal regimes, and to truncate the interval from initiation of therapy to cure, early, accurate identification of the causative agent is crucial, making a fast identification to the species level after culture or direct from clinical specimens a necessity. A correct fungal identification can only be achieved if the phylogenetic relationships between the pathogenic organisms and their taxonomy is resolved. Gene detection is more reproducible than detection of morphological and biochemical differences. The proposal focuses on the establishment of an accurate phylogenetic system of pathogenic fungi, which will form the basis of a universally applicable molecular identification system and to develop a molecular reference database for human pathogenic fungi. This project will contribute sequence data of pathogenic fungi to the Tree of Life project. This project unites expertise in classical mycology, molecular biology, bioinformatics and infectious diseases, to develop an accurate phylogeny of medically important fungi, providing a unique opportunity to establish a quality controlled reference database accessible via the world-wide-web. This will provide a faster and more accurate ID of pathogenic fungi, which will lead to better clinical treatment.Read moreRead less
Nuclear structure and function in the nucleated planctomycete bacterium Gemmata obscuriglobus: a third cell plan for living organisms? The project will contribute knowledge of how cells such as those of animals and plants evolved from bacterial components. Origins of cell nuclei and pores in nuclear membranes will be studied via the simple eukaryote-like nucleus of the planctomycete Gemmata obscuriglobus, from Australian freshwater. Simple nuclear pore-like structures of G. obscuriglobus will he ....Nuclear structure and function in the nucleated planctomycete bacterium Gemmata obscuriglobus: a third cell plan for living organisms? The project will contribute knowledge of how cells such as those of animals and plants evolved from bacterial components. Origins of cell nuclei and pores in nuclear membranes will be studied via the simple eukaryote-like nucleus of the planctomycete Gemmata obscuriglobus, from Australian freshwater. Simple nuclear pore-like structures of G. obscuriglobus will help understanding nucleus function in animal cells, and such pores will give insight into 'minimal' composition needed for cell nuclei, and allow design of biological nanopores. The origin of the nucleus is a major problem in biology, and an Australian contribution to its solution will achieve international recognition. Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0344009
Funder
Australian Research Council
Funding Amount
$300,000.00
Summary
South Australian Regional Facility for Molecular Evolution and Ecology. Adelaide and Flinders Universities and their partners, the South Australian Museum and the South Australian Research and Development Institute, seek to enhance the throughput and efficiency of molecular genotyping for the large amount of research undertaken in Adelaide in the general areas of molecular evolution and ecology, and to enhance long-term storage and accessibility of the valuable biological materials used for geno ....South Australian Regional Facility for Molecular Evolution and Ecology. Adelaide and Flinders Universities and their partners, the South Australian Museum and the South Australian Research and Development Institute, seek to enhance the throughput and efficiency of molecular genotyping for the large amount of research undertaken in Adelaide in the general areas of molecular evolution and ecology, and to enhance long-term storage and accessibility of the valuable biological materials used for genotyping. This will be achieved by developing a new fully integrated, jointly managed regional facility, that serves all relevant research groups in South Australia.Read moreRead less
The Origin and Evolution of the Animal Phyla inferred from Analysis of Multiple-Gene Data. Australia has recently begun an extensive research programme in the genomics of our flora and fauna. The enormous amounts of data that emerge from such research are highly complex, but they hold the key to understanding how biological organisms change over time. Our research will untangle that data to answer fundamental, unanswered questions in modern science: How did the animal groups originate? How are ....The Origin and Evolution of the Animal Phyla inferred from Analysis of Multiple-Gene Data. Australia has recently begun an extensive research programme in the genomics of our flora and fauna. The enormous amounts of data that emerge from such research are highly complex, but they hold the key to understanding how biological organisms change over time. Our research will untangle that data to answer fundamental, unanswered questions in modern science: How did the animal groups originate? How are they related to each other? How is biodiversity changing? The answers to these questions and the new analytical tools we will develop will put Australia firmly on the international "map" of Bioinformatics.Read moreRead less
Molecular Cell Biology and Comparative Genomics Of Planctomycetes and Verrucomicrobia In Relation To Evolution Of Cytoskeletal Proteins and Membrane-bounded Compartments. Planctomycetes and verrucomicrobia are evolutionarily distinct groups of bacteria which possess unusual cell structure and which share some significant genes important in cell biology with eukaryotes e.g. in verrucomicrobia the cytoskeleton protein tubulin. These bacteria are important for understanding the transition from no ....Molecular Cell Biology and Comparative Genomics Of Planctomycetes and Verrucomicrobia In Relation To Evolution Of Cytoskeletal Proteins and Membrane-bounded Compartments. Planctomycetes and verrucomicrobia are evolutionarily distinct groups of bacteria which possess unusual cell structure and which share some significant genes important in cell biology with eukaryotes e.g. in verrucomicrobia the cytoskeleton protein tubulin. These bacteria are important for understanding the transition from non-nucleated cells with simple cell division to nucleated cells with chromosome separation via cytoskeletal protein movement.The project will compare genomes of of planctomycetes and verrucomicrobia to determine their relationship, determine whether the tubulin homolog of verrucomicrobia can form cytoskeleton structures, and characterize the cytoskeleton of ammonium-oxidizing planctomycetes used in wastewater treatment.Read moreRead less
Structural reorganization of the hymenopteran mitochondrial genome. This study will be the first detailed investigation of the evolution of mt genome reorganization, and as such it will identify the processes that shape the evolution of a molecule widely used to interpret phylogeny. A description of the processes that lead to mt genome reorganization will have a substantial impact on our understanding in two areas of mt biology; (1) the discovery of new molecular phenomena that impact on the or ....Structural reorganization of the hymenopteran mitochondrial genome. This study will be the first detailed investigation of the evolution of mt genome reorganization, and as such it will identify the processes that shape the evolution of a molecule widely used to interpret phylogeny. A description of the processes that lead to mt genome reorganization will have a substantial impact on our understanding in two areas of mt biology; (1) the discovery of new molecular phenomena that impact on the organization and evolution of this genome, and (2) the interpretation of its phylogenetic content. It will establish our research group as a leader in the field of evolutionary genetics. Training of high quality students, with exposure to international researchers, will be a significant component of this program.Read moreRead less
Cultivating numerically significant soil bacteria. The vast majority of soil bacteria have not been able to be studied in the laboratory because they cannot be grown outside the soil. They are therefore termed unculturable. Most of these belong to groups that are completely unstudied. Advances made in the Janssen lab have overcome this impediment to laboratory cultivation of numerically abundant and globally distributed soil bacteria. This project will develop these advances to generate simple a ....Cultivating numerically significant soil bacteria. The vast majority of soil bacteria have not been able to be studied in the laboratory because they cannot be grown outside the soil. They are therefore termed unculturable. Most of these belong to groups that are completely unstudied. Advances made in the Janssen lab have overcome this impediment to laboratory cultivation of numerically abundant and globally distributed soil bacteria. This project will develop these advances to generate simple and widely applicable methods to enable many of the previously unculturable soil bacteria to be studied. This will allow assessments of their ecological roles and biotechnological potentials to be made.Read moreRead less
Molecular phylogeny and biodiversity of the plant family Rutaceae: evidence for Australian-New Caledonian biogeography. The results will contribute biological evidence for geological models of the evolution of New Caledonia and the Southwest Pacific region. Analyses will also contribute to improvement of methods and data interpretation in the field of historical biogeography. The molecular phylogeny will be the foundation for a new predictive classification of Australian Rutaceae at the level ....Molecular phylogeny and biodiversity of the plant family Rutaceae: evidence for Australian-New Caledonian biogeography. The results will contribute biological evidence for geological models of the evolution of New Caledonia and the Southwest Pacific region. Analyses will also contribute to improvement of methods and data interpretation in the field of historical biogeography. The molecular phylogeny will be the foundation for a new predictive classification of Australian Rutaceae at the level of tribes and genera. Improved phylogenetic classification underpins the delivery of biodiversity research, goods and services in Australia. Outcomes of improved taxonomy include species identification for ecological studies, identification of rare species, geographic areas of high conservation value and plants for horticulture.Read moreRead less
A predictive phylogenetic classification for Australian acacias and their tropical legume relatives worldwide. The results of this project will provide the foundation for a new predictive classification of Australian acacias and their relatives, among tropical legumes. Improved phylogenetic classification will underpin the delivery of biodiversity research, goods and services in Australia and neighbouring tropical countries. Outcomes of improved taxonomy include species identification and spec ....A predictive phylogenetic classification for Australian acacias and their tropical legume relatives worldwide. The results of this project will provide the foundation for a new predictive classification of Australian acacias and their relatives, among tropical legumes. Improved phylogenetic classification will underpin the delivery of biodiversity research, goods and services in Australia and neighbouring tropical countries. Outcomes of improved taxonomy include species identification and species selection in various industries and environmental programs. Acacias and legumes are used in agro-forestry, as shade trees for crops, fuel wood, stock feed, nitrogen fixation, planting for land reclamation in arid and salinity-affected areas, and as new plantation timber products. Read moreRead less
Resolving the classification and evolutionary history of the eucalypts. This collaborative project addresses the need to achieve a stable classification of the eucalypts, Australia's most ecologically and commercially important trees. The industry partners are lead agencies with responsibility for classification and identification of biodiversity, delivering taxonomic knowledge for end-users. Despite molecular studies in the last decade, the phylogenetic relationships of a number of the major ....Resolving the classification and evolutionary history of the eucalypts. This collaborative project addresses the need to achieve a stable classification of the eucalypts, Australia's most ecologically and commercially important trees. The industry partners are lead agencies with responsibility for classification and identification of biodiversity, delivering taxonomic knowledge for end-users. Despite molecular studies in the last decade, the phylogenetic relationships of a number of the major subgroups of the eucalypts are unknown. We will sequence new regions of DNA and combine this with morphological data to resolve the relationships of the eucalypt lineages, and hence their classification. Phylogenetic analysis also aids identification of high-value areas for conservation of relictual species.Read moreRead less