Non-Covalent Interactions Probed by Velocity Map Imaging. Our research program concerns non-covalent interactions, which are of fundamental importance in a range of areas including condensed phase chemistry, the folding of large molecules, biomolecule shape, host-guest chemistry and molecular self-assembly. We probe non-covalent interactions through studies of van der Waals molecules, which involve two or more molecules or atoms held together by non-covalent forces. Our group has developed new ....Non-Covalent Interactions Probed by Velocity Map Imaging. Our research program concerns non-covalent interactions, which are of fundamental importance in a range of areas including condensed phase chemistry, the folding of large molecules, biomolecule shape, host-guest chemistry and molecular self-assembly. We probe non-covalent interactions through studies of van der Waals molecules, which involve two or more molecules or atoms held together by non-covalent forces. Our group has developed new experimental methodologies for determining the strength of intermolecular interactions and these will be used to study the effect of 3-body interactions. Because of their role in chemical reaction, we will also undertake detailed studies of complexes involving radical species.Read moreRead less
WAVES IN THE MIDDLE ATMOSPHERE. Atmospheric waves have a profound influence in the atmosphere. A unique network of radars in the southern hemisphere will be used to study wave processes in the upper atmosphere. The project will investigate causes of wave variability, wave sources and wave-wave interactions and will involve satellite measurements and international collaboration. Results will guide the development and testing of schemes that incorporate the effects of small-scale waves in numerica ....WAVES IN THE MIDDLE ATMOSPHERE. Atmospheric waves have a profound influence in the atmosphere. A unique network of radars in the southern hemisphere will be used to study wave processes in the upper atmosphere. The project will investigate causes of wave variability, wave sources and wave-wave interactions and will involve satellite measurements and international collaboration. Results will guide the development and testing of schemes that incorporate the effects of small-scale waves in numerical weather and climate models. Outcomes from the project will have application in modelling climate change.Read moreRead less
Estimating genotype-environment interaction using genomic information. This project aims to develop statistical methods that can explore genotype–environment interaction at the genomic level using genome-wide single nucleotide polymorphisms or sequence data. It plans to estimate how the effects of genetic variants change with changing environmental conditions and how overall genetic variance changes due to changing effects in specific gene regions. It plans to deliver statistical models and meth ....Estimating genotype-environment interaction using genomic information. This project aims to develop statistical methods that can explore genotype–environment interaction at the genomic level using genome-wide single nucleotide polymorphisms or sequence data. It plans to estimate how the effects of genetic variants change with changing environmental conditions and how overall genetic variance changes due to changing effects in specific gene regions. It plans to deliver statistical models and methods and an efficient algorithm implemented in software, which would broadly benefit the field of complex trait genetics. Methods to estimate genotype–environment interaction effects at the genomic level would help elucidate complex biological systems, including human genetic response to changing environmental factors and the potential adaptation of animals to changing environmental conditions.Read moreRead less
Reconstructing wheat evolution using ancient DNA. The domestication of wild grasses by farmers was a step change in human history; it led to the emergence of modern cereals and with them, western civilisation. This project will apply modern DNA sequencing methods to 5000-year-old cereal seeds to reconstruct the history of wheat, barley and other crops, and identify lost ancient forms and diversity.
European impact on Eastern Australian coastal lakes: understanding pre-impact conditions and post-settlement modification. Coastal lakes are vulnerable to European impact, yet little is known about how they have been altered in the last 200 years. For example: Are blue-green algal blooms a natural occurrence in coastal lakes? How long have freshwater lakes been isolated from the ocean? Preliminary results indicate that, at key sites, blue-green algae were more abundant before European settlement ....European impact on Eastern Australian coastal lakes: understanding pre-impact conditions and post-settlement modification. Coastal lakes are vulnerable to European impact, yet little is known about how they have been altered in the last 200 years. For example: Are blue-green algal blooms a natural occurrence in coastal lakes? How long have freshwater lakes been isolated from the ocean? Preliminary results indicate that, at key sites, blue-green algae were more abundant before European settlement and that coastal lakes thought to be permanently fresh have been exposed to the ocean within the past 200 years. By documenting change at representative sites, the project will identify which lake types are most vulnerable to impact. Results from the project will ensure that costly restoration efforts are not misplaced.Read moreRead less
Whole-genome multivariate reaction norm model for complex traits. This project aims to develop a multivariate whole-genome genotype-covariate correlation and interaction model that can be applied to a wide range of existing genome-wide association study (GWAS) datasets. Genotype-covariate correlation and interaction (GCCI) are fundamental in biology but there is no standard approach to disentangle interaction from correlation in the whole-genome analyses. This project will address the key featur ....Whole-genome multivariate reaction norm model for complex traits. This project aims to develop a multivariate whole-genome genotype-covariate correlation and interaction model that can be applied to a wide range of existing genome-wide association study (GWAS) datasets. Genotype-covariate correlation and interaction (GCCI) are fundamental in biology but there is no standard approach to disentangle interaction from correlation in the whole-genome analyses. This project will address the key feature in biology, which relates to dissecting the complex mechanism of association and interaction. The proposed statistical model implemented in a context of a novel design based on multiple GWAS data sets is a paradigm shifting-tool with applications to multiple industries.Read moreRead less
Novel Mass Spectrometric Approaches to the Study of Protein-Protein Interactions. Protein-protein interactions mediate all fundamental cellular processes, yet the structural complexity of protein assemblies mean they are often difficult to characterise using traditional analytical methods. This project will develop and demonstrate novel mass spectrometric approaches towards a molecular level description of the structure and interactions of biological protein complexes, which in turn may underpi ....Novel Mass Spectrometric Approaches to the Study of Protein-Protein Interactions. Protein-protein interactions mediate all fundamental cellular processes, yet the structural complexity of protein assemblies mean they are often difficult to characterise using traditional analytical methods. This project will develop and demonstrate novel mass spectrometric approaches towards a molecular level description of the structure and interactions of biological protein complexes, which in turn may underpin the rational design of drugs for the treatment of a range of human health conditions. This project will also provide training of young researchers to the highest international standards in mass spectrometry and protein science, for benefit to Australian industry and research.Read moreRead less
Nanotribology and Nanorheometry: A Fundamental Study of the Dynamic Interactions of Particles and Surfaces at the Molecular Level. Friction and deformation occur from the mutual motion and interaction of microscopic particles and surfaces. This research aims to develop new theories and measurement techniques for these non-equilibrium phenomena by combining mathematical analysis and numerical computations with dynamic force measurement, surface modification, and surface characterisation on nanom ....Nanotribology and Nanorheometry: A Fundamental Study of the Dynamic Interactions of Particles and Surfaces at the Molecular Level. Friction and deformation occur from the mutual motion and interaction of microscopic particles and surfaces. This research aims to develop new theories and measurement techniques for these non-equilibrium phenomena by combining mathematical analysis and numerical computations with dynamic force measurement, surface modification, and surface characterisation on nanometre and molecular length scales. These insights and data will be critically important in designing low-friction surfaces that save energy and wear, in developing nanoscopic probes for the mechanical and structural properties of soft polymeric and bio-materials, and in making high performance coatings that control adhesion and particle aggregation in technologically advanced applications.Read moreRead less
Energetics and dynamics of solvated biologically relevant molecules using liquid microjet and ion imaging technologies. The shape of many biomolecules in solution plays a critical role in determining their biological activity and function. It is known that the bonds that form between the biomolecules and the water solvent control this shape. However, very little is known about the strength and structure of these bonds at different sites around the biomolecule. Many experiments have informed u ....Energetics and dynamics of solvated biologically relevant molecules using liquid microjet and ion imaging technologies. The shape of many biomolecules in solution plays a critical role in determining their biological activity and function. It is known that the bonds that form between the biomolecules and the water solvent control this shape. However, very little is known about the strength and structure of these bonds at different sites around the biomolecule. Many experiments have informed us about the strength of the bonds, others have told us where the bonds occur. This project will provide both pieces of information for the first time, allowing us to better understand, and therefore control, biological function. This work will assist in the development of new biotechnology processes, especially in the emerging area of proteomics.Read moreRead less
Laser Spectroscopic Studies of Non-Covalent Solute-Solvent Interactions Involving Non-Volatile Biomolecules in the Gas Phase. Solute-solvent interactions play a critical role in determining whether or not many biomolecules display biological activity in solution. Making use of our novel liquid microjet injection technology, we will generate micro-solvated gas phase biomolecules by rapid laser desorption from the surface of a liquid jet (here, micro-solvation describes up to ~10 solvent molecule ....Laser Spectroscopic Studies of Non-Covalent Solute-Solvent Interactions Involving Non-Volatile Biomolecules in the Gas Phase. Solute-solvent interactions play a critical role in determining whether or not many biomolecules display biological activity in solution. Making use of our novel liquid microjet injection technology, we will generate micro-solvated gas phase biomolecules by rapid laser desorption from the surface of a liquid jet (here, micro-solvation describes up to ~10 solvent molecules attached to a solute). Following desorption, laser spectroscopy will be used to directly characterise the non-volatile biomolecular solute-solvent topography. This project will create a new application of laser science to study biophysical and biochemical processes that remain difficult, if not impossible, to explore using traditional techniques.Read moreRead less