A tale of two genomes: integrating mitochondrial biogenesis into the cell cycle and metabolic control. The human genome is cordoned into two distinct compartments in our cells. Most genes are in the nucleus, while a distinct set of genes are held within our mitochondria. Using yeast as a model organism, this project will provide a holistic view of how expression of the two genomes is coordinated.
Role of suppressor of cytokine signalling proteins (SOCS3) in defective muscle repair and ageing. Old muscles are slower and weaker than young muscles, they are injured more easily and they repair less successfully. This proposal investigates the role of SOCS3-signalling in muscle repair, ultimately to improve healing and to promote healthy ageing that will enable older Australians to enjoy a better quality of life.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE200100183
Funder
Australian Research Council
Funding Amount
$950,000.00
Summary
Protein Quantitation Centre of South Australia renewal for Systems Biology. This application aims to renew Mass Spectrometry (MS) instrumentation to characterise and quantify Biomolecules towards a better understanding of biological processes. UniSA, Uni Adelaide, Flinders have established the Protein Quantitation Centre of South Australia (PQCSA) in 2013 through an ARC LIEF lead by CI Hoffmann and this application will renew and expand MS capacity towards metabolites, glycans and lipids. This ....Protein Quantitation Centre of South Australia renewal for Systems Biology. This application aims to renew Mass Spectrometry (MS) instrumentation to characterise and quantify Biomolecules towards a better understanding of biological processes. UniSA, Uni Adelaide, Flinders have established the Protein Quantitation Centre of South Australia (PQCSA) in 2013 through an ARC LIEF lead by CI Hoffmann and this application will renew and expand MS capacity towards metabolites, glycans and lipids. This will enable researchers in South Australia to work towards a full understanding of biological processes and towards expanding their knowledge to Systems Biology. Expected outcome of the projects are multiple interdisciplinary collaborations between the CI's and should provide significant benefits in research outputs.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE120100794
Funder
Australian Research Council
Funding Amount
$375,000.00
Summary
Revealing dynamic mechanisms controlling pluripotency in mammalian stem cells and embryos. Every cell of our mature bodies originates from 'pluripotent' cells present in the early mammalian embryo. These cells can be captured and grown in plastic dishes. The project will use imaging methods to reveal how gene regulatory molecules control pluripotent cells in the embryo and in culture.
Metalloproteomics: A new piece of the systems biology puzzle. Systems biology uses advanced analytical technology to study the complex chemistry of the living cell. Many cellular functions are the result of chemical reactions involving metalloproteins, which are notoriously difficult to study due to the weak bonds between metal and protein that is not normally amenable to traditional proteomic approaches. In partnership with the leading analytical manufacturer Agilent Technologies, this project ....Metalloproteomics: A new piece of the systems biology puzzle. Systems biology uses advanced analytical technology to study the complex chemistry of the living cell. Many cellular functions are the result of chemical reactions involving metalloproteins, which are notoriously difficult to study due to the weak bonds between metal and protein that is not normally amenable to traditional proteomic approaches. In partnership with the leading analytical manufacturer Agilent Technologies, this project aims to adapt and apply advanced mass spectrometry to the study of metalloproteins, developing new methods for studying hundreds of molecules in single experiments. Using the C. elegans model organism the project aims to showcase the importance of metals in biology and develop new solutions for the $2.9 billion proteomics industry.Read moreRead less
Empirical and computational solutions for multi-omics single-cell assays. Emerging single-cell sequencing technologies are transforming molecular cell biology, but identifying novel cell types and their functions requires the integration of highly heterogeneous data. The development of computational methods able to extract biologically relevant results is hindered by the lack of high-quality datasets. This project aims to develop novel sequencing methodologies and generate data to drive our dime ....Empirical and computational solutions for multi-omics single-cell assays. Emerging single-cell sequencing technologies are transforming molecular cell biology, but identifying novel cell types and their functions requires the integration of highly heterogeneous data. The development of computational methods able to extract biologically relevant results is hindered by the lack of high-quality datasets. This project aims to develop novel sequencing methodologies and generate data to drive our dimension reduction multivariate method developments for data integration. By combining in silico and in vivo approaches, the project is anticipated to benefit scientists willing to work in cutting-edge single-cell research by providing useful protocols and tools to generate novel insights in cell biology. Read moreRead less
Systems-level characterization of scaffold protein signalling networks. The PEAK family of cell signalling scaffolds regulate cellular responses critical for normal development and physiology. This project will adopt a ‘holistic’ approach to characterizing their mechanism and function, integrating experimental and mathematical approaches and developing predictive computational models. It aims to generate fundamental new knowledge in cell, computational and synthetic biology with broad relevance ....Systems-level characterization of scaffold protein signalling networks. The PEAK family of cell signalling scaffolds regulate cellular responses critical for normal development and physiology. This project will adopt a ‘holistic’ approach to characterizing their mechanism and function, integrating experimental and mathematical approaches and developing predictive computational models. It aims to generate fundamental new knowledge in cell, computational and synthetic biology with broad relevance that will foster establishment of new international linkages. This research will also identify strategies for engineering novel scaffolds that re-program cellular behaviour towards specific, applied outcomes, with potential benefit for the fields of synthetic biology, bioengineering and biotechnology.Read moreRead less
Defining the molecular switches that govern discrete cellular fates. This project aims to elucidate how mammalian cells exploit the same molecular machinery to perform completely distinct jobs. While the repurposing of proteins by cells seems widespread, the mechanisms by which this occurs remains largely undefined. The project expects to generate new knowledge in the areas of cell signalling and systems biology, with important implications for many multi-functional proteins. It will utilise a h ....Defining the molecular switches that govern discrete cellular fates. This project aims to elucidate how mammalian cells exploit the same molecular machinery to perform completely distinct jobs. While the repurposing of proteins by cells seems widespread, the mechanisms by which this occurs remains largely undefined. The project expects to generate new knowledge in the areas of cell signalling and systems biology, with important implications for many multi-functional proteins. It will utilise a highly innovative and interdisciplinary approach that tightly integrates mathematical modelling and biological experiments. The expected outcomes will aid strategies for reprogramming cells towards a desired phenotype, which will bring significant benefits to the fields of synthetic biology and bioengineering.Read moreRead less
Mapping and defining inter-organ cross talk during exercise. This project aims to examine precisely how organs communicate and interact. These interactions are particularly important during exercise, when continued movement demands intricate organ communication, and have major ramifications for the whole organism as it ages. Precisely how this communication takes place is unclear, but we now know that the movement of cargo with extracellular vesicles (EVs) plays an integral role in organ to orga ....Mapping and defining inter-organ cross talk during exercise. This project aims to examine precisely how organs communicate and interact. These interactions are particularly important during exercise, when continued movement demands intricate organ communication, and have major ramifications for the whole organism as it ages. Precisely how this communication takes place is unclear, but we now know that the movement of cargo with extracellular vesicles (EVs) plays an integral role in organ to organ communication. This project expects to build upon unprecedented recent developments we have made in the biology of inter-organ communication via EVs. The expected outcomes will have broad impact across life science and biotechnology.Read moreRead less
Characterization of the dark metabolome of eukaryotic cells. The project aims to investigate the full metabolic potential of a group of eukaryotic organisms using advanced analytical and computational techniques. It will identify novel metabolites and enzyme activities that are currently not predicted from genome annotations. Expected outcomes of the project include the delineation of new metabolic processes that are common to all eukaryotes, the characterization of new enzymes families, and the ....Characterization of the dark metabolome of eukaryotic cells. The project aims to investigate the full metabolic potential of a group of eukaryotic organisms using advanced analytical and computational techniques. It will identify novel metabolites and enzyme activities that are currently not predicted from genome annotations. Expected outcomes of the project include the delineation of new metabolic processes that are common to all eukaryotes, the characterization of new enzymes families, and the generation of comprehensive metabolic databases. An improved understanding of cellular metabolism will provide direct benefits in biotechnology, food production, environmental monitoring and the diagnosis and treatment of human metabolic and infectious diseases. Read moreRead less