New approaches to unravelling post-translational controls operating on the cyanobacterial carbon dioxide concentrating mechanism. Marine blue-green algae contribute to global primary productivity but their carbon dioxide acquisition processes are poorly understood. The project will employ mutagenesis and genome sequencing to discover the controls that regulate carbon dioxide uptake processes required for efficient photosynthesis and use this data to aid in engineering crop plants that use less w ....New approaches to unravelling post-translational controls operating on the cyanobacterial carbon dioxide concentrating mechanism. Marine blue-green algae contribute to global primary productivity but their carbon dioxide acquisition processes are poorly understood. The project will employ mutagenesis and genome sequencing to discover the controls that regulate carbon dioxide uptake processes required for efficient photosynthesis and use this data to aid in engineering crop plants that use less water.Read moreRead less
Adapting cyanobacterial bicarbonate transporters for application in crop improvement. Marine blue-green algae contribute to global primary productivity but their carbon dioxide (CO2) acquisition processes are poorly understood. The project will determine protein structure and regulatory controls present in two classes of bicarbonate transporters required for efficient photosynthesis, and use this data towards the engineering crop plants that use less water.
Functional genomics of light stress resistance in the model organism Chlamydomonas: combining molecular genetics, transcriptome and proteome analysis. This project aims at combining molecular genetics, transcriptome and proteome analysis to identify genes and pathways underlying high light stress tolerance in previously isolated mutants of the chlorophyte Chlamydomonas reinhardtii. Comprehensive profiles of transcriptome-proteome linkage will be constructed without the complications of multicel ....Functional genomics of light stress resistance in the model organism Chlamydomonas: combining molecular genetics, transcriptome and proteome analysis. This project aims at combining molecular genetics, transcriptome and proteome analysis to identify genes and pathways underlying high light stress tolerance in previously isolated mutants of the chlorophyte Chlamydomonas reinhardtii. Comprehensive profiles of transcriptome-proteome linkage will be constructed without the complications of multicellularity for this unicellular photosynthetic model organism. We will establish a public proteome reference database and provide new microarrays and molecular markers beneficial for research in Chlamydomonas. We expect to advance understanding of high light resistance mechanisms so that it will eventually be applicable to improve productivity in crop plants growing under various environmental stress conditions.Read moreRead less
Novel photoprotective mechanisms and functional biodiversity of high light tolerance in the model alga Chlamydomonas. Most plants have limited capacity to avoid high light (HL) stress which commonly accompanies drought and high temperature stress. We will identify novel genes and proteins that underlie diverse mechanisms of photoprotection in unique very high light resistant (VHLR) mutants in the alga Chlamydomonas and develop new tools to screen other plants for these attributes. Depending on p ....Novel photoprotective mechanisms and functional biodiversity of high light tolerance in the model alga Chlamydomonas. Most plants have limited capacity to avoid high light (HL) stress which commonly accompanies drought and high temperature stress. We will identify novel genes and proteins that underlie diverse mechanisms of photoprotection in unique very high light resistant (VHLR) mutants in the alga Chlamydomonas and develop new tools to screen other plants for these attributes. Depending on progress, we expect to express them in the higher plant Arabidopsis as a first step towards utilization of VHLR genes for crop improvement. Understanding the mechanisms conferring HL photoprotection is a research priority in plant sciences and will further strengthen Australia's innovative contributions to the internationally networked Chlamydomonas Genome Project.Read moreRead less
REdefining metabolic Schemes and Pathways In plant leaf REspiration. Leaf respiration-related metabolism in terrestrial vegetation liberates considerable amounts of carbon dioxide, ammonia and hydrogen sulphide into the atmosphere. Such gaseous losses are detrimental to biomass production but respiration also sustains nutrient assimilation and biosyntheses. This project aims to describe flux patterns in respiratory metabolism and disentangle interactions with other pathways such as photorespirat ....REdefining metabolic Schemes and Pathways In plant leaf REspiration. Leaf respiration-related metabolism in terrestrial vegetation liberates considerable amounts of carbon dioxide, ammonia and hydrogen sulphide into the atmosphere. Such gaseous losses are detrimental to biomass production but respiration also sustains nutrient assimilation and biosyntheses. This project aims to describe flux patterns in respiratory metabolism and disentangle interactions with other pathways such as photorespiration and nitrogen assimilation. It will exploit stable isotopes to quantify metabolic partitioning and show coordination between major processes. It will establish key mechanisms by which respiration dictates plant carbon balance and contributes to identifying metabolic bottle-necks in plant primary production.Read moreRead less
MicroRNA control of gene expression and development in plants. Controlling a gene's activity is fundamental in biotechnology, from dissecting a gene's function to introducing desired traits in crop plants. Recently a new class of genes have been identified that are 'master-regulators' able to control the activity of many genes, and through this, they can ultimately control how an organism develops and functions. By understanding how these genes operate and the extent of their influence, not only ....MicroRNA control of gene expression and development in plants. Controlling a gene's activity is fundamental in biotechnology, from dissecting a gene's function to introducing desired traits in crop plants. Recently a new class of genes have been identified that are 'master-regulators' able to control the activity of many genes, and through this, they can ultimately control how an organism develops and functions. By understanding how these genes operate and the extent of their influence, not only will we gain greater insights into the gene regulatory networks required for multicellular life, but how we may develop methods of controlling gene activity that will have tremendous biotechnological applications.Read moreRead less
Chloroplast pigments as developmental signals. This project will define roles of chloroplast pigments in cellular signaling, plant growth and development. Effective communication between the chloroplast and the nucleus is essential for maintaining viability and productivity of the cell. Similarly, long-distance signaling is required for coordinated growth and development and we hypothesise that pigments also have importance in this role. Our proposal brings together the multidisciplinary skills ....Chloroplast pigments as developmental signals. This project will define roles of chloroplast pigments in cellular signaling, plant growth and development. Effective communication between the chloroplast and the nucleus is essential for maintaining viability and productivity of the cell. Similarly, long-distance signaling is required for coordinated growth and development and we hypothesise that pigments also have importance in this role. Our proposal brings together the multidisciplinary skills and resources of six investigators to define how certain carotenoids and chlorophyll precursors regulate gene expression and to determine the identity of what is most likely a carotenoid derivative with hormonal properties that controls lateral branch growth.Read moreRead less
The key to making root nodules - new tricks for old hormones. This project aims to use and develop new chemical and microscopy-based tools to test whether the cell-type specific plant hormone changes induced by rhizobia in legumes can be triggered in non-legumes. Most crop plants rely on fossil fuel-derived nitrogen fertilisers, while legumes benefit from biologically-fixed nitrogen through a symbiosis with rhizobia bacteria that initiate nodules. It is not understood why non-legumes cannot form ....The key to making root nodules - new tricks for old hormones. This project aims to use and develop new chemical and microscopy-based tools to test whether the cell-type specific plant hormone changes induced by rhizobia in legumes can be triggered in non-legumes. Most crop plants rely on fossil fuel-derived nitrogen fertilisers, while legumes benefit from biologically-fixed nitrogen through a symbiosis with rhizobia bacteria that initiate nodules. It is not understood why non-legumes cannot form this symbiosis. This project aims to apply detailed knowledge of nodulation in model legumes to a phylogenetically diverse range of nodulating and non-nodulating plant species. This is expected to give new insight into the evolution of nodulation and advance the long held goal of extending nodulation to non-legumes.Read moreRead less
THE MECHANISMS OF PHOTOPROTECTION IN PLANTS - A GENOMICS AND PHOTOPHYSICAL APPROACH. Coping with adverse environmental conditions is central to plant survival in nature so understanding the photoprotective mechanisms of light acclimation is important for crop improvement. Therefore, effective acclimatory mechanisms at whole plant, cellular and molecular levels are essential to accommodate short and long-term exposure to potentially photodamaging full sunlight and environmental stresses, such as ....THE MECHANISMS OF PHOTOPROTECTION IN PLANTS - A GENOMICS AND PHOTOPHYSICAL APPROACH. Coping with adverse environmental conditions is central to plant survival in nature so understanding the photoprotective mechanisms of light acclimation is important for crop improvement. Therefore, effective acclimatory mechanisms at whole plant, cellular and molecular levels are essential to accommodate short and long-term exposure to potentially photodamaging full sunlight and environmental stresses, such as drought and temperature extremes that lead to plant death or greatly reduced crop yields due to free radical damage. This project brings together a unique cross-disciplinary expertise in biophysics, biochemistry, physiology and genomics to elucidate the known mechanisms and identify unknown factors in photoprotection.Read moreRead less
Plant microRNA systems: investigating slicing versus translational repression and the development of an anti-viral defence mechanism. The ability to specifically switch off genes has revolutionised biotechnological approaches, from fighting diseases to developing superior crops, however the underlying mechanism is poorly understood, limiting its application in practice. Outcomes from this project will provide such knowledge, allowing its precise use in diverse applications.