Australian Centre For Vertebrate Mutation Detection (ACVMD)
Funder
National Health and Medical Research Council
Funding Amount
$1,611,794.00
Summary
Over the last 20 years, generation and analysis of genetically modified animals has proven to be an important step in the transition from in vitro studies of gene function to in vivo studies and eventually clinical research. The remarkable parallels between the human, mouse and zebrafish genomes means that there are now many examples of mutations that cause or modify disease in humans, and which lead to similar phenotypes when present in mice and zebrafish. Until recently, the prime method of in ....Over the last 20 years, generation and analysis of genetically modified animals has proven to be an important step in the transition from in vitro studies of gene function to in vivo studies and eventually clinical research. The remarkable parallels between the human, mouse and zebrafish genomes means that there are now many examples of mutations that cause or modify disease in humans, and which lead to similar phenotypes when present in mice and zebrafish. Until recently, the prime method of introducing mutations into specific genes of interest in the mouse (although still unavailable in the fish) was via homologous recombination, and the principal classes of mutations induced were large deletions or insertions. This type of mutation rarely occurs in humans. Rather, point mutations and single-nucleotide polymorphisms are the prevalent form of genetic variation. An alternative approach to the development of mouse models with the more relevant point mutations is TILLING (Targeting Induced Local Lesions IN Genomes). The goal of this Enabling Grant is to make TILLING technology accessible to the Australian research community and in doing so promote movement of research from the in vitro setting into animal models of disease.Read moreRead less
The Effects Of Estrogen-Responsive B Box Protein On Retinoid Sensitivity In Cancer And Its Significance In Development
Funder
National Health and Medical Research Council
Funding Amount
$82,421.00
Summary
Although effective, many cancer drugs often lead to side effects, especially in children. New therapies are needed that specifically target cancer cells while leaving normal cells unaffected. I am studying a novel protein (EBBP) which I believe has an important role in cancer cell growth. By studying EBBP I aim to be able to increase the effectiveness of the low toxic chemotherapy retinoic acid without increased side effects, as well as understand the functional role of EBBP in cancer cells.
My background is in the study of human molecular genetic disease, and my interest has evolved to the analysis of embryonic development using the mouse as a model system. My particular interest is in the molecular mechanisms governing limb and craniofacial
Regulation Of Inflammation And Thrombosis By Endothelial Protein C Receptor And Thrombomodulin In Xenograft Rejection
Funder
National Health and Medical Research Council
Funding Amount
$35,085.00
Summary
Pig-to-human organ transplantation may be the solution to the human organ shortage crisis. However, cross-species organ transplantation invariably results in graft destruction and rejection. Genetically modified mice expressing anti-rejection proteins will be tested to assess their effects and benefits on grafts. If such genes improve graft outcome, pigs with similar genetic modifications will be generated for the purposes of pig-to-primate organ transplantation studies.