Protecting cereal grain development at high temperatures. This project aims to investigate new temperature-responsive factors that regulate cereal grain development to protect grain production under heat stress. The new research will leverage international collaborations with access to cutting-edge genetic and technological resources, and refine novel X-ray imaging techniques in Australia, to observe how temperature affects flower structure and function in barley and rice. Favourable mutations t ....Protecting cereal grain development at high temperatures. This project aims to investigate new temperature-responsive factors that regulate cereal grain development to protect grain production under heat stress. The new research will leverage international collaborations with access to cutting-edge genetic and technological resources, and refine novel X-ray imaging techniques in Australia, to observe how temperature affects flower structure and function in barley and rice. Favourable mutations that optimise plant yield and fitness will be defined and explored in other, more complex, cereals such as wheat. Expected outcomes will be fundamental breakthroughs in understanding how plants respond to, and buffer, the effects of heat to lead to translational breeding strategies that bolster grain yield.Read moreRead less
Targeting chloroplasts to enhance crop salt tolerance. Yield losses in crop plants due to increasingly saline soils are linked to the effects of salt on chloroplasts. By comparing chloroplast water- and salt-transport mechanisms of closely related salt-loving and salt-sensitive plants, this Fellowships aims to discover how chloroplasts maintain function in saline conditions. Novel biophysics and molecular techniques will be used to characterise transporters in model plants, and proof-of-concept ....Targeting chloroplasts to enhance crop salt tolerance. Yield losses in crop plants due to increasingly saline soils are linked to the effects of salt on chloroplasts. By comparing chloroplast water- and salt-transport mechanisms of closely related salt-loving and salt-sensitive plants, this Fellowships aims to discover how chloroplasts maintain function in saline conditions. Novel biophysics and molecular techniques will be used to characterise transporters in model plants, and proof-of-concept complementation experiments aim to confer salt tolerance on sensitive plants. These fundamental insights are likely to lead to rapid, step-change improvements in salt tolerance, especially in agriculturally relevant crops, to benefit Australia’s agri-industry and ensure food security in the future.Read moreRead less
Remodelling encapsulin nanocages to help enhance plant carbon fixation. Nature has evolved mechanisms in microbial systems to improve photosynthetic efficiency by saturating the enzyme Rubisco with carbon dioxide. These carbon concentrating mechanisms are genetically complex, precluding successful introduction into crops. Our simpler approach is to use encapsulins, a new source of robust bacterial pore-containing nanocages made from a single gene. This project will optimise the development of sy ....Remodelling encapsulin nanocages to help enhance plant carbon fixation. Nature has evolved mechanisms in microbial systems to improve photosynthetic efficiency by saturating the enzyme Rubisco with carbon dioxide. These carbon concentrating mechanisms are genetically complex, precluding successful introduction into crops. Our simpler approach is to use encapsulins, a new source of robust bacterial pore-containing nanocages made from a single gene. This project will optimise the development of synthetic encapsulin-Rubisco carbon-fixing nanoreactors and transform them into leaf chloroplasts to test their impact on plant photosynthesis and growth. Our genetically simpler solution will aid ongoing global efforts to deliver overdue step change improvements in agricultural productivity.Read moreRead less
Improving plant reproductive success under heat stress: A sweet approach. This project aims to determine how genetic manipulation of cell wall invertase (CWIN) activity could regulate pollen germination, elongation and fruit set under heat stress using tomato as a model. Plant reproductive processes are highly susceptible to heat stress, which often leads to pollination failure and fruit and seed abortion, hence irreversible yield loss. Research has established that CWIN-mediated sugar metabolis ....Improving plant reproductive success under heat stress: A sweet approach. This project aims to determine how genetic manipulation of cell wall invertase (CWIN) activity could regulate pollen germination, elongation and fruit set under heat stress using tomato as a model. Plant reproductive processes are highly susceptible to heat stress, which often leads to pollination failure and fruit and seed abortion, hence irreversible yield loss. Research has established that CWIN-mediated sugar metabolism and signaling may play crucial roles in pollen growth and fruit set under heat stress. The intended outcome is the generation of critical knowledge that will advance understanding on reproductive development under heat stress, thereby providing significant benefits, such as novel ideas and solutions for improving crop yield.Read moreRead less
Can altered sugar sensing improve crop productivity? This project aims at genetically manipulating sugar sensing pathways in the model C4 grass Setaria viridis, and at replacing sugar sensors in the model C3 crop Oryza sativa (rice) with those from S. viridis. This project expects to elucidate the impact of altered sugar perception on crop photosynthesis and yield. Expected outcomes includes advancing a novel “pull” approach to improve yield in C3 crops by using C4-like sugar sensors to reduce f ....Can altered sugar sensing improve crop productivity? This project aims at genetically manipulating sugar sensing pathways in the model C4 grass Setaria viridis, and at replacing sugar sensors in the model C3 crop Oryza sativa (rice) with those from S. viridis. This project expects to elucidate the impact of altered sugar perception on crop photosynthesis and yield. Expected outcomes includes advancing a novel “pull” approach to improve yield in C3 crops by using C4-like sugar sensors to reduce feedback regulation of photosynthesis which in turn limits productivity. This is in contrast to previous ‘push’ approaches aimed at directly increasing photosynthesis. Hence, this project provides significant benefits by contributing to the next green revolution needed to lift agricultural yields.Read moreRead less
Using venoms to map critical and evolutionary conserved vulnerabilities. We have developed and applied new functional genomic approaches to study venom evolution. Using CRISPR screening, we find that unrelated venoms act on cells by exploiting the same vulnerabilities. By functionally mapping these vulnerabilities for all venom classes, we can begin to develop universal venom antidotes. Conversely, much of what we know about venom mechanisms comes from a small percentage of the biodiversity with ....Using venoms to map critical and evolutionary conserved vulnerabilities. We have developed and applied new functional genomic approaches to study venom evolution. Using CRISPR screening, we find that unrelated venoms act on cells by exploiting the same vulnerabilities. By functionally mapping these vulnerabilities for all venom classes, we can begin to develop universal venom antidotes. Conversely, much of what we know about venom mechanisms comes from a small percentage of the biodiversity within a venom, and we have developed genomic tools to study the venom “dark matter”. This work will lead to the full molecular characterisation of venom biodiversity, and new venom components will be useful for research or as novel medicines.Read moreRead less
Identification of novel plant transporters responsible for sucrose efflux. This project aims to clone and functionally characterise previously unknown membrane proteins that facilitate high rates of sucrose efflux from cells located at key transport bottlenecks regulating sucrose transport throughout the plant body and hence plant productivity. These aims will be realised through employing systems specifically designed to clone and functionally characterise sucrose efflux proteins encoded in pla ....Identification of novel plant transporters responsible for sucrose efflux. This project aims to clone and functionally characterise previously unknown membrane proteins that facilitate high rates of sucrose efflux from cells located at key transport bottlenecks regulating sucrose transport throughout the plant body and hence plant productivity. These aims will be realised through employing systems specifically designed to clone and functionally characterise sucrose efflux proteins encoded in plant genomes. Expected outcomes will be an understanding of sucrose transport throughout the plant body, build a valuable international partnership and open up new biotechnological opportunities to improve crop yield.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE240100068
Funder
Australian Research Council
Funding Amount
$931,950.00
Summary
Australian Advanced Metabolic Signal Discovery, and Imaging Platform. This proposal aims to establish an Australian Advanced Metabolic Signal Discovery and Imaging platform. The platform consists of an ultra-high resolution gas chromatography mass spectrometer and an imaging mass spectrometry upgrade for a second existing high resolution mass spectrometer. The facility will break barriers currently limiting discovery and localisation of metabolic changes during plant and animal development under ....Australian Advanced Metabolic Signal Discovery, and Imaging Platform. This proposal aims to establish an Australian Advanced Metabolic Signal Discovery and Imaging platform. The platform consists of an ultra-high resolution gas chromatography mass spectrometer and an imaging mass spectrometry upgrade for a second existing high resolution mass spectrometer. The facility will break barriers currently limiting discovery and localisation of metabolic changes during plant and animal development under environmental stress; integral chemical signals exchanged in host-microbe interactions; and volatile signatures linked to ecosystem health and developmental anomalies in animals. Results will inform innovative strategies to enhance biological adaptation, climate resilience and plant, animal, and ecosystem health.Read moreRead less
Testing links between genomic and morphological evolutionary rates. This project aims to identify, understand, and characterise patterns of evolutionary rates across different levels of biological variation. The project expects to generate knowledge about the tempo and mode of evolution by using a phylogenetic approach to test fundamental models of evolutionary rates, including the link between rates of genomic and morphological evolution. Expected outcomes of this project include detailed insig ....Testing links between genomic and morphological evolutionary rates. This project aims to identify, understand, and characterise patterns of evolutionary rates across different levels of biological variation. The project expects to generate knowledge about the tempo and mode of evolution by using a phylogenetic approach to test fundamental models of evolutionary rates, including the link between rates of genomic and morphological evolution. Expected outcomes of this project include detailed insights into the tempo and mode of macroevolution, better modelling of genomic and phenotypic evolution, and improved design of studies in evolutionary genomics. Benefits of the project include greater understanding of the evolutionary processes that have generated the diversity of the Australian biota.Read moreRead less
In depth characterisation of the gamma delta T cell immune synapse. This project aims to comprehensively characterise the activation principles of gamma delta T cells. These cells have an understudied but central role in vertebrate immunity and development. A missing piece of the puzzle is how gamma delta T cells sense stress and how this signal leads to activation. Expected outcomes include the generation of fundamental knowledge in immunology and structural biology. This proposal uses high-ski ....In depth characterisation of the gamma delta T cell immune synapse. This project aims to comprehensively characterise the activation principles of gamma delta T cells. These cells have an understudied but central role in vertebrate immunity and development. A missing piece of the puzzle is how gamma delta T cells sense stress and how this signal leads to activation. Expected outcomes include the generation of fundamental knowledge in immunology and structural biology. This proposal uses high-skilled techniques, including cryo-electron microscopy and single-molecule imaging and holds ancillary benefits to postgraduate students. Anticipated outcomes include influential publications, building a critical mass of expertise in Australia and fostering international collaborations with Australia at the epicentre.Read moreRead less