Single molecule sensing on nanopillars: Reading complex molecular circuits. This project aims to develop an entirely new nanotechnology to visualise dynamic molecular circuits in real time, and within any biological sample as small as a single cell. This project expects to generate new knowledge in the field of cell biology and sensor technology, using innovative nanofabrication and nanoscopic fluid flows to advance understanding of the emerging field of single protein molecule interactions in c ....Single molecule sensing on nanopillars: Reading complex molecular circuits. This project aims to develop an entirely new nanotechnology to visualise dynamic molecular circuits in real time, and within any biological sample as small as a single cell. This project expects to generate new knowledge in the field of cell biology and sensor technology, using innovative nanofabrication and nanoscopic fluid flows to advance understanding of the emerging field of single protein molecule interactions in cellular pathways. Expected outcomes include a universal technology platform to detect single molecules in single cells, with potential to deliver valuable intellectual property of commercial interest and economic benefit through technological advancements.Read moreRead less
Trapping and Watching Biomolecular Complexes near Nanopores. This project aims to develop a technology to trap and interrogate nanosized molecular complexes in their natural state. Nanosized complexes in water provide the core machinery of biological systems and require detailed understanding to help unravel fundamental biological mechanisms. A prototype of a methodology has been developed to trap and interrogate nanoscaled objects as small as 190 nanometres within a nanopore device. By performi ....Trapping and Watching Biomolecular Complexes near Nanopores. This project aims to develop a technology to trap and interrogate nanosized molecular complexes in their natural state. Nanosized complexes in water provide the core machinery of biological systems and require detailed understanding to help unravel fundamental biological mechanisms. A prototype of a methodology has been developed to trap and interrogate nanoscaled objects as small as 190 nanometres within a nanopore device. By performing research to fully understand, miniaturise and develop this method further, this project aims to enable the quantitative observation of nanoscaled biological machinery involved in cell-to-cell communication and DNA unfolding. Such a technology platform may have applications in areas such as biology, biotechnology and advanced materials.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE210101666
Funder
Australian Research Council
Funding Amount
$395,588.00
Summary
Engineering nanoparticles with enhanced adhesion at the nano-bio interfaces. This project aims to develop a next-generation adhesive nanoparticle platform through in-depth understandings of nanoparticle interactions with bio-interfaces. This project expects to generate new knowledge in the multidisciplinary research field at nano-bio-interfaces by using a recently developed nano-colloidal probe technology, instructing the rational design of nanoparticles with enhanced interface adhesive properti ....Engineering nanoparticles with enhanced adhesion at the nano-bio interfaces. This project aims to develop a next-generation adhesive nanoparticle platform through in-depth understandings of nanoparticle interactions with bio-interfaces. This project expects to generate new knowledge in the multidisciplinary research field at nano-bio-interfaces by using a recently developed nano-colloidal probe technology, instructing the rational design of nanoparticles with enhanced interface adhesive properties. Expected outcomes include a family of adhesive nanoparticles designed for nanopesticide and animal feed applications, with the potential to deliver valuable intellectual property of commercial interest and economic benefit through technology advancement.Read moreRead less
DNA exhibits new self-assembled structures due to clustered DNA methylation. This project aims to develop a technology to investigate detailed epigenetic patterns in DNA by directly interrogating the physical properties of DNA polymers in their native state. Epigenetics controls whether genes and genetic programs are turned on or off in living systems. The project will build on a recent discovery that key physical properties of native DNA polymers are strongly influenced by epigenetic patterns c ....DNA exhibits new self-assembled structures due to clustered DNA methylation. This project aims to develop a technology to investigate detailed epigenetic patterns in DNA by directly interrogating the physical properties of DNA polymers in their native state. Epigenetics controls whether genes and genetic programs are turned on or off in living systems. The project will build on a recent discovery that key physical properties of native DNA polymers are strongly influenced by epigenetic patterns created by living organisms. By fully understanding this phenomenon, this project aims to provide new tools for the study of epigenetics with broad potential applications in the life sciences, biotechnology and nanotechnology.Read moreRead less
Industrial Transformation Training Centres - Grant ID: IC210100056
Funder
Australian Research Council
Funding Amount
$3,975,864.00
Summary
ARC Training Centre for Next-Gen Technologies in Biomedical Analysis . The Centre for Next-Gen Technologies in Biomedical Analysis will deliver workforce trained in the development of transformative technologies that will rapidly expand the Australian pharmaceutical, diagnostic and defence sector. The university-industry partnership will increase Australia’s manufacturing capability by fast tracking screening, by integrating 3D printing, advanced sensing, big data analytics, machine learning an ....ARC Training Centre for Next-Gen Technologies in Biomedical Analysis . The Centre for Next-Gen Technologies in Biomedical Analysis will deliver workforce trained in the development of transformative technologies that will rapidly expand the Australian pharmaceutical, diagnostic and defence sector. The university-industry partnership will increase Australia’s manufacturing capability by fast tracking screening, by integrating 3D printing, advanced sensing, big data analytics, machine learning and artificial intelligence for the delivery of optimal solutions in diagnosis, treatment and wellbeing. The centre will deliver training in Industry 4.0 skills which will boost early-stage scale-up and accelerate the sector’s supply chain, which is pivotal for the Australian industries to maintain a competitive edge. Read moreRead less
Nano-reactors: Protein cages as reusable scaffolds for designer enzymes. This project aims to develop robust protein cages derived from the coats of viruses to contain heat-stable P450 enzymes, for use as specialised protein bio-catalysts in chemical industries. A valuable chemical precursor of renewable bio-plastics will be produced from seed oils by enzymes, reducing the use of fossil fuels. This synthetic biology approach combines biotechnology, nanotechnology and protein engineering to estab ....Nano-reactors: Protein cages as reusable scaffolds for designer enzymes. This project aims to develop robust protein cages derived from the coats of viruses to contain heat-stable P450 enzymes, for use as specialised protein bio-catalysts in chemical industries. A valuable chemical precursor of renewable bio-plastics will be produced from seed oils by enzymes, reducing the use of fossil fuels. This synthetic biology approach combines biotechnology, nanotechnology and protein engineering to establish a plant-based platform biotechnology for using enzymes as catalysts to make high-value molecules. The project aims to show how to engineer clean, sustainable chemistry in designer nano-environments. This should make synthetic processes more sustainable and enhance advanced chemical manufacturing in Australia.Read moreRead less
Next generation hybrid nanomaterials: bispecific antibody-targeted polymers. This project aims to develop and optimise a novel platform technology that will assist in the development of hybrid materials consisting of nanomaterials and biomolecules, which form the basis of many commercial diagnostic devices. A novel antibody, MIL38, will provide the test bed for the technology, which will aim to deliver a platform that is stable under physiological conditions and that enables facile conjugation o ....Next generation hybrid nanomaterials: bispecific antibody-targeted polymers. This project aims to develop and optimise a novel platform technology that will assist in the development of hybrid materials consisting of nanomaterials and biomolecules, which form the basis of many commercial diagnostic devices. A novel antibody, MIL38, will provide the test bed for the technology, which will aim to deliver a platform that is stable under physiological conditions and that enables facile conjugation of nanomaterials with antibodies. This project has the potential to rapidly improve the ligation process between synthetic nanomaterials and biologics, leading to more efficient synthesis of targeted diagnostics. This would provide a significant commercial advantage for any nanomaterials developed for the field, and specifically for this project, expedite translation of MIL38.Read moreRead less
Establishing Design Principles Of Polymers For Intracellular Delivery . Engineered polymers have played a central role in the field of bionanotechnology by enabling targeted nanoscale cell interactions. Progress in the field of intracellular delivery is currently affected by a major bottleneck due to the absence of effective polymers that is applicable across the range of bimolecular cargoes. In essence depending on the type of cargo: DNA, RNA or protien, the polymer needs programmability. The l ....Establishing Design Principles Of Polymers For Intracellular Delivery . Engineered polymers have played a central role in the field of bionanotechnology by enabling targeted nanoscale cell interactions. Progress in the field of intracellular delivery is currently affected by a major bottleneck due to the absence of effective polymers that is applicable across the range of bimolecular cargoes. In essence depending on the type of cargo: DNA, RNA or protien, the polymer needs programmability. The limited tunability of traditional polymers agents makes them unsuitable for this particular application. The multidisciplinary project addresses this significant problem by engineering novel sequences of defined polymer based nanoscale agents to achieve efficient delivery in cells.Read moreRead less
Bioengineering self-assembly of innovative core-shell nanomaterials . This project aims to generate new knowledge in nanoscale bioengineering. It expects to develop a disruptive platform technology for design and manufacture of advanced nanomaterials to provide solutions for unmet needs in industry. It will explore an innovative bioengineering concept that merges biopolymer synthesis with virus-like particle self-assembly to produce innovative tunable core-shell nanomaterials. Expected outcomes ....Bioengineering self-assembly of innovative core-shell nanomaterials . This project aims to generate new knowledge in nanoscale bioengineering. It expects to develop a disruptive platform technology for design and manufacture of advanced nanomaterials to provide solutions for unmet needs in industry. It will explore an innovative bioengineering concept that merges biopolymer synthesis with virus-like particle self-assembly to produce innovative tunable core-shell nanomaterials. Expected outcomes are the development of advanced techniques for design and manufacture of innovate nanomaterials with enhanced stability and performance. This innovative platform technology for precision engineering of high-performance nanomaterials should provide significant benefits for biotechnological and agricultural industries.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE150100067
Funder
Australian Research Council
Funding Amount
$390,000.00
Summary
The Vevo 2100 Micro-ultrasound plus LAZR Photoacoustic Imaging Platform . The Vevo 2100 micro-ultrasound plus LAZR photoacoustic imaging platform: The Vevo/LAZR ultrasound/photoacoustic imaging facility will allow researchers to achieve multiple outcomes: to visualise and quantify, non-invasively, tissue and molecular structures; the movement and behaviour of cells; and the delivery patterns of administered imaging dyes and nanoparticles in mouse models and reconstructed tissues. This will enabl ....The Vevo 2100 Micro-ultrasound plus LAZR Photoacoustic Imaging Platform . The Vevo 2100 micro-ultrasound plus LAZR photoacoustic imaging platform: The Vevo/LAZR ultrasound/photoacoustic imaging facility will allow researchers to achieve multiple outcomes: to visualise and quantify, non-invasively, tissue and molecular structures; the movement and behaviour of cells; and the delivery patterns of administered imaging dyes and nanoparticles in mouse models and reconstructed tissues. This will enable researchers to obtain anatomical, functional, physiological and molecular data simultaneously and in real-time, with resolution down to 40 micrometres. This will translate into both user efficiency and laboratory cost effectiveness, but more significantly is expected to result in greater understanding of fundamental mechanisms regulating the body's cell and tissue functions.Read moreRead less