Improving the efficiency of CRISPR gene editing in cells. Human red blood cells are well-characterised and the globin gene locus is a model system for the study of gene regulation. Gene editing technologies and delivery tools are evolving rapidly and the globin gene locus is the perfect model for gene editing optimisation. This collaboration between UNSW Sydney and CSL aims to bring together our combined expertise and new technologies to develop an optimal platform for genetic modification in a ....Improving the efficiency of CRISPR gene editing in cells. Human red blood cells are well-characterised and the globin gene locus is a model system for the study of gene regulation. Gene editing technologies and delivery tools are evolving rapidly and the globin gene locus is the perfect model for gene editing optimisation. This collaboration between UNSW Sydney and CSL aims to bring together our combined expertise and new technologies to develop an optimal platform for genetic modification in a red blood cell line. Simultaneously, this project aims to generate fundamental insights into mechanisms of human gene regulation. The technological and biological outcomes of this project will be of benefit for future gene editing applications.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE140100114
Funder
Australian Research Council
Funding Amount
$560,000.00
Summary
High Throughput Cell Genomics Centre. High throughput cell genomics centre: This project will establish a high throughput cell genomics centre comprising a Fluidigm C1™ Single-Cell AutoPrep and BioMark™ HD system providing researchers with the most innovative approach to single cell and small population analyses. The instruments will enable the unique capability to conduct single cell transcriptome analysis and high throughput gene expression, SNP genotyping and copy number variation analysis as ....High Throughput Cell Genomics Centre. High throughput cell genomics centre: This project will establish a high throughput cell genomics centre comprising a Fluidigm C1™ Single-Cell AutoPrep and BioMark™ HD system providing researchers with the most innovative approach to single cell and small population analyses. The instruments will enable the unique capability to conduct single cell transcriptome analysis and high throughput gene expression, SNP genotyping and copy number variation analysis as well as validation of next generation sequencing data. The information generated is crucial to advancing knowledge in important research fields including infection and immunity, regenerative medicine, immune responses, biomarker discovery, drug discovery, biotechnology and agriculture.Read moreRead less
Decoding regulatory RNA function in bacteria. All complex biological processes in bacterial cells appear to utilise regulatory small RNAs to control gene expression, but we lack a systems-level understanding of their functions and mechanisms of control. This proposal aims to address this fundamental knowledge gap using machine learning and cutting-edge, systems-level techniques to determine how small RNA sequence and structure determines function. Small RNAs have been found to control a broad ra ....Decoding regulatory RNA function in bacteria. All complex biological processes in bacterial cells appear to utilise regulatory small RNAs to control gene expression, but we lack a systems-level understanding of their functions and mechanisms of control. This proposal aims to address this fundamental knowledge gap using machine learning and cutting-edge, systems-level techniques to determine how small RNA sequence and structure determines function. Small RNAs have been found to control a broad range of traits including metabolism, biofilm formation, antibiotic tolerance, and virulence. The work proposed here will enhance our ability to predict and control bacterial gene expression with potential future impacts on bioproduction, synthetic biology, and veterinary and medical microbiology.Read moreRead less
Investigating the biogenesis and function of circular RNAs in the brain. Circular RNAs (circRNAs) are e a novel class of RNA molecules produced in a wide spectrum of eukaryotic organisms, from yeast to humans. Their expression is particularly high in the nervous system in the fruit fly, mouse and humans. What mechanisms are responsible for the tissue-specific enrichment of circular RNA expression? What are the consequences of circular RNA production on gene expression? The overall goal of the pr ....Investigating the biogenesis and function of circular RNAs in the brain. Circular RNAs (circRNAs) are e a novel class of RNA molecules produced in a wide spectrum of eukaryotic organisms, from yeast to humans. Their expression is particularly high in the nervous system in the fruit fly, mouse and humans. What mechanisms are responsible for the tissue-specific enrichment of circular RNA expression? What are the consequences of circular RNA production on gene expression? The overall goal of the proposed project is to elucidate these important aspects of circRNA biogenesis. Specifically, the project aims to (a) discover proteins that regulate circRNA expression, (b) elucidate how circRNA expression interacts with alternative splicing, and (c) identify circular RNAs that play regulatory roles in gene expression. Read moreRead less
How does the noncoding genome regulate gene expression in the human brain? The non-coding genome is recognized as a major player in orchestrating gene expression in higher eukaryotes. This project aims to identify regions of the human genome that are important for gene expression during neuronal differentiation and depolarisation (i.e. neural enhancers), and to investigate their evolutionary properties. The roles of non-coding DNA in regulating the dynamic gene expression patterns underlying com ....How does the noncoding genome regulate gene expression in the human brain? The non-coding genome is recognized as a major player in orchestrating gene expression in higher eukaryotes. This project aims to identify regions of the human genome that are important for gene expression during neuronal differentiation and depolarisation (i.e. neural enhancers), and to investigate their evolutionary properties. The roles of non-coding DNA in regulating the dynamic gene expression patterns underlying complex human brain functions remains to be elucidated. By combining transcriptome quantification and bioinformatics methods, this project will close an important knowledge gap in our understanding of transcriptional regulation underlying human brain function. This will provide benefits such as the potential to influence public health policy including in cognitive functions and aging.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0453561
Funder
Australian Research Council
Funding Amount
$336,706.00
Summary
Integrated Precision Machining of Complex Profiles. High precision and high speed machining is of central importance to the development of a wide range of frontier technologies such as precision manufacturing, nano-materials fabrication, semiconductor and composite processing, vehicle production and steel structure manufacturing. The research significance has been well established by the world-leading projects and achievements. The proposed facility has distinct features and capability of high l ....Integrated Precision Machining of Complex Profiles. High precision and high speed machining is of central importance to the development of a wide range of frontier technologies such as precision manufacturing, nano-materials fabrication, semiconductor and composite processing, vehicle production and steel structure manufacturing. The research significance has been well established by the world-leading projects and achievements. The proposed facility has distinct features and capability of high loop-stiffness, high repeatability, universal profiling, organic integration of key machining operations, and great flexibility of both low and high speeds. These unique characteristics will enable the innovative development of many research programs which will otherwise be impossible to achieve.Read moreRead less
High-Performance Microwave and Millimetre Wave Antennae Based on Multi-layer Periodic Structures. Broadband communication has created a rapidly growing market for innovative microwave communication systems such as WiFi and WiMAX. Millimetre-wave technology is expected to deliver the next leap in communication technology with much faster wireless links for 3D TV etc. By developing innovative, low-cost, planar antennas with high performance, the proposed research will create opportunities for Aust ....High-Performance Microwave and Millimetre Wave Antennae Based on Multi-layer Periodic Structures. Broadband communication has created a rapidly growing market for innovative microwave communication systems such as WiFi and WiMAX. Millimetre-wave technology is expected to deliver the next leap in communication technology with much faster wireless links for 3D TV etc. By developing innovative, low-cost, planar antennas with high performance, the proposed research will create opportunities for Australian industry to compete in this growing global market with advanced, cost-effective, microwave and millimetre-wave products. The Australian research community will benefit from new methods, techniques and trained researchers, while Australian consumers will benefit from improved quality and low cost of services.Read moreRead less
Hybrid-resonator antennas for wireless communication networks. The rapid emergence of modern wireless communication systems has led to a requirement for small, lightweight antennas. In this project, a new, broadband, low-cost, small and lightweight antenna architecture will be developed for wireless systems. The new architecture is based on a novel hybrid-resonator concept: a dielectric resonator tightly coupled to a metal patch resonator. The rapid design and optimisation of new antennas will b ....Hybrid-resonator antennas for wireless communication networks. The rapid emergence of modern wireless communication systems has led to a requirement for small, lightweight antennas. In this project, a new, broadband, low-cost, small and lightweight antenna architecture will be developed for wireless systems. The new architecture is based on a novel hybrid-resonator concept: a dielectric resonator tightly coupled to a metal patch resonator. The rapid design and optimisation of new antennas will be achieved by developing several new theoretical methods. Antennas targeted for the Unlicensed National Information Infrastructure (UNII) band (5-6 GHz) commercial wireless communication systems will be designed, fabricated, tested and integrated with the systems.
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Integration of Wireless LAN and GPRS/UMTS Cellular Systems. Integration of wireless local area networks with cellular mobile systems provides a feasible, reliable, and cost-effective way for high-speed mobile Internet access in wide area networks. In this project in collaboration with Australia's second largest telecommunications company; Optus, we will develop network architecture and essential access and mobility techniques for such a system. The project's outcomes will enable Optus to expand ....Integration of Wireless LAN and GPRS/UMTS Cellular Systems. Integration of wireless local area networks with cellular mobile systems provides a feasible, reliable, and cost-effective way for high-speed mobile Internet access in wide area networks. In this project in collaboration with Australia's second largest telecommunications company; Optus, we will develop network architecture and essential access and mobility techniques for such a system. The project's outcomes will enable Optus to expand its broadband mobile Internet service to all parts and population of the country including rural and regional areas and subsequently export the breakthrough technology resulted from the project to overseas.Read moreRead less