Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0239218
Funder
Australian Research Council
Funding Amount
$340,000.00
Summary
Proteomics facility for biotechnology research in WA. The aim of this application is to establish a fully functional proteomics facility to underpin the future research of many groups in four WA institutions. While molecular biological research has thus far concentrated on molecular genetics, it is now vital to directly examine the patterns of proteins expressed by cells to understand their molecular mechanisms. Individual WA researchers have already ventured into proteomics, utilising facilit ....Proteomics facility for biotechnology research in WA. The aim of this application is to establish a fully functional proteomics facility to underpin the future research of many groups in four WA institutions. While molecular biological research has thus far concentrated on molecular genetics, it is now vital to directly examine the patterns of proteins expressed by cells to understand their molecular mechanisms. Individual WA researchers have already ventured into proteomics, utilising facilities located elsewhere in Australia. The reliance on other groups to conduct their proteomics, which are rapidly becoming basic techniques for competitive molecular biological research, is a serious obstacle to biotechnological research in WA.Read moreRead less
Protein Complexes and Supercomplexes of Plant Organelles. Ample parts of plant primary metabolism occur in subcellular structures called mitochondria, plastids and peroxisomes. They are vital for plant growth and development and are central to the early success of germinating and growing seedlings. This project intends to analyze the protein complexes and supercomplexes within these organelles using state of the art instrumentation and technologies. Findings from this research have the potential ....Protein Complexes and Supercomplexes of Plant Organelles. Ample parts of plant primary metabolism occur in subcellular structures called mitochondria, plastids and peroxisomes. They are vital for plant growth and development and are central to the early success of germinating and growing seedlings. This project intends to analyze the protein complexes and supercomplexes within these organelles using state of the art instrumentation and technologies. Findings from this research have the potential to directly flow into the plant biotechnology industry and could assist the future development of Australian agriculture through genetic improvements. The expertise developed by this work will ensure that Australia is well placed to meet future needs and to generally improve agricultural technology. Read moreRead less
Decoding germination defects that threaten global wheat production. Wheat is a major commodity in Australia. Sprouting damage represents a major global threat to wheat production and food security. This project will explore the genetic and molecular mechanisms underpinning pre-harvest sprouting (PHS) and late-maturity amylase (LMA). This project will apply transcriptomics and proteomics to measure the expression of the biomolecules associated with PHS and LMA, generating fundamental knowledge of ....Decoding germination defects that threaten global wheat production. Wheat is a major commodity in Australia. Sprouting damage represents a major global threat to wheat production and food security. This project will explore the genetic and molecular mechanisms underpinning pre-harvest sprouting (PHS) and late-maturity amylase (LMA). This project will apply transcriptomics and proteomics to measure the expression of the biomolecules associated with PHS and LMA, generating fundamental knowledge of grain molecular physiology that addresses a significant knowledge gap. The project will deliver tools capable of differentiating these conditions, thereby minimising economic losses. A better understanding of the genetic basis of PHS and LMA will lay the foundation for advanced breeding aiming to eliminate these. Read moreRead less
Molecular mechanisms governing the role of calcium in aluminium toxicity and tolerance in plants. Aluminium is the most important yield-limiting factor in acid soils throughout the world. The problem of aluminium toxicity is aggravated by continuous acidification of arable land. Mechanisms of aluminium toxicity in plant cells are poorly understood. The present project seeks to elucidate the molecular basis of the interaction between intracellular calcium homeostasis and plasma membrane potential ....Molecular mechanisms governing the role of calcium in aluminium toxicity and tolerance in plants. Aluminium is the most important yield-limiting factor in acid soils throughout the world. The problem of aluminium toxicity is aggravated by continuous acidification of arable land. Mechanisms of aluminium toxicity in plant cells are poorly understood. The present project seeks to elucidate the molecular basis of the interaction between intracellular calcium homeostasis and plasma membrane potential in aluminium toxicity to plants. Knowledge of primary triggers of aluminium toxicity will pay off in a breeding programme aimed at selecting crop genotypes with increased resistance to aluminium toxicity.Read moreRead less
The regulation and role of dual targeted proteins in plant cells. Plant cells are the factories that provide the food we eat, the air we breath, play a critical role in a balanced environment and provide energy in a sustainable manner. The varied use of plants and plant products is underpinned by an understanding of biochemistry that takes place in plant cells. This proposal is aimed at understanding how some proteins function in more than one location in the multi-compartmentalised plant cell b ....The regulation and role of dual targeted proteins in plant cells. Plant cells are the factories that provide the food we eat, the air we breath, play a critical role in a balanced environment and provide energy in a sustainable manner. The varied use of plants and plant products is underpinned by an understanding of biochemistry that takes place in plant cells. This proposal is aimed at understanding how some proteins function in more than one location in the multi-compartmentalised plant cell biochemical factory. The knowledge that is generated from this research can be used to underpin innovative use of plants within Australia's plant based industries. Furthermore it provides a rich intellectual training environment for students and postdoctoral researchers.Read moreRead less
Role of nitrogen and sulphur nutrition in determining quantity and quality of oil in canola seed. Canola is the second most important crop in Australia, with exports worth $1 billion a year. However, competitiveness of Australian canola on the world markets is hampered by low and inconsistent oil content. Nitrogen fertilisation increases seed yield and seed protein content, but decreases oil content. This project will elucidate regulation by nitrogen and sulphur (the other important nutrient in ....Role of nitrogen and sulphur nutrition in determining quantity and quality of oil in canola seed. Canola is the second most important crop in Australia, with exports worth $1 billion a year. However, competitiveness of Australian canola on the world markets is hampered by low and inconsistent oil content. Nitrogen fertilisation increases seed yield and seed protein content, but decreases oil content. This project will elucidate regulation by nitrogen and sulphur (the other important nutrient in canola growth) of protein and oil biosynthesis in developing canola grain. The knowledge generated in this project will allow optimisation of canola agronomy and more effective breeding for increased nitrogen- and sulphur-use efficiency, seed yields and oil content in canola, thus enhancing the competitiveness of Australian canola on the world markets.Read moreRead less
Role of intracellular calcium homeostasis and aluminium transport across the plasma membrane in aluminium toxicity to plants. Aluminium is the most important yield-limiting factor in acid soils throughout the world. The problem of aluminium toxicity is aggravated by continuous acidification of arable land. Mechanisms of aluminium toxicity in plant cells are poorly understood. The present project seeks to elucidate the molecular basis of the interaction between intracellular calcium homeostasis, ....Role of intracellular calcium homeostasis and aluminium transport across the plasma membrane in aluminium toxicity to plants. Aluminium is the most important yield-limiting factor in acid soils throughout the world. The problem of aluminium toxicity is aggravated by continuous acidification of arable land. Mechanisms of aluminium toxicity in plant cells are poorly understood. The present project seeks to elucidate the molecular basis of the interaction between intracellular calcium homeostasis, cytosolic pH and aluminium uptake across the plasma membrane in aluminium toxicity to plants. Knowledge of primary triggers of aluminium toxicity will pay off in a breeding programme aimed at selecting crop genotypes with increased resistance to aluminium toxicity.Read moreRead less
Aluminium uptake across the root-cell plasma membrane. Aluminium toxicity limits crop growth in acid soils that occupy about 24 million hectares of agricultural land in Australia. Liming can increase pH of the surface soil, but is frequently too expensive in the low-input Australian agriculture. Surface-applied lime is poorly effective in ameliorating subsoil acidity, and incorporating lime deep into the profile is prohibitively expensive and technically difficult. Hence, Al-resistant crop culti ....Aluminium uptake across the root-cell plasma membrane. Aluminium toxicity limits crop growth in acid soils that occupy about 24 million hectares of agricultural land in Australia. Liming can increase pH of the surface soil, but is frequently too expensive in the low-input Australian agriculture. Surface-applied lime is poorly effective in ameliorating subsoil acidity, and incorporating lime deep into the profile is prohibitively expensive and technically difficult. Hence, Al-resistant crop cultivars are important part of sustainable farming in Australia. This project will characterise early triggers of Al toxicity in plants, providing a foundation for increasing Al resistance in crop cultivars. Understanding the physiological basis of Al toxicity will lead to improved crop breeding strategies.Read moreRead less
Tightening the phosphorus cycle for grain legumes. Using unique core collections of chickpea, soybean and peanut with diverse genetic backgrounds, this project aims to unravel the mechanisms underlying high phosphorus-use efficiency (PUE) at morphological, physiological, biochemical and molecular levels in three major legume crops. Reduced levels of phosphorus and phytate in seeds will improve seed quality for humans and livestock and dramatically reduce phosphorus-fertiliser inputs. The identif ....Tightening the phosphorus cycle for grain legumes. Using unique core collections of chickpea, soybean and peanut with diverse genetic backgrounds, this project aims to unravel the mechanisms underlying high phosphorus-use efficiency (PUE) at morphological, physiological, biochemical and molecular levels in three major legume crops. Reduced levels of phosphorus and phytate in seeds will improve seed quality for humans and livestock and dramatically reduce phosphorus-fertiliser inputs. The identification of traits and genes associated with high PUE will allow transfer of key traits into commercial cultivars using molecular breeding approaches. Cultivars with improved PUE will enable reduced phosphate fertiliser input and loss of phosphate in runoff from agricultural systems.Read moreRead less
Phosphorus - A Key Factor in the Development of Novel Perennial Herbaceous Deep-rooted Pasture Legumes. This research aims at the development of urgently needed perennial pasture legumes, to expand perennial pasture options for southern Australia beyond lucerne. The development of new deep-rooted perennial pasture legumes has enormous potential to improve nutrient and water use over large areas of agricultural land. Benefits in terms of reducing soil erosion and acidification are also likely. An ....Phosphorus - A Key Factor in the Development of Novel Perennial Herbaceous Deep-rooted Pasture Legumes. This research aims at the development of urgently needed perennial pasture legumes, to expand perennial pasture options for southern Australia beyond lucerne. The development of new deep-rooted perennial pasture legumes has enormous potential to improve nutrient and water use over large areas of agricultural land. Benefits in terms of reducing soil erosion and acidification are also likely. An understanding of the responses of new perennial legumes to soil phosphorus is a prerequisite for the development of new perennial farming systems. Overall, both environmental and financial benefits will accrue at scales ranging from individual farmers and rural industries through to the general community.Read moreRead less