Circular Plant Proteins with Pharmaceutical Applications. The proposed research will develop methods for using plants as protein production factories. Initially I will use plants to create engineered cyclotides that incorporate peptides with proven therapeutic activity against cancer and multiple sclerosis. Successful production of therapeutic proteins in plants will benefit Australians by making treatments for these and other diseases more accessible. It also has the potential for a major econo ....Circular Plant Proteins with Pharmaceutical Applications. The proposed research will develop methods for using plants as protein production factories. Initially I will use plants to create engineered cyclotides that incorporate peptides with proven therapeutic activity against cancer and multiple sclerosis. Successful production of therapeutic proteins in plants will benefit Australians by making treatments for these and other diseases more accessible. It also has the potential for a major economic benefit from the sales of Australian-based drugs. This proposal will also provide outstanding research training for graduate students in multidisciplinary methods that constitute state-of the-art structural and plant molecular biology.Read moreRead less
Advancing our understanding of plant responses to low phosphorus availability beyond the transcriptome. Phosphorus is essential for plant growth. Plants have evolved mechanisms to cope with the poor availability of phosphorus in many soils. This project will improve Australia's knowledge of how plants alter the expression of genes and proteins to activate their mechanisms for coping with poor phosphorus availability in some soils.
Is transport of miRNAs essential for plant development? This project will provide knowledge of how a new class of biologically active molecule (micro RNA) regulates expression of genes at sites in the plant that are critical for growth and development. MicroRNAs are believed to influence the size and shape of plants, how rapidly they grow and how well they produce and fill seeds. These molecules are part of a group of bioactive signals that move throughout the plant, functioning like hormones bu ....Is transport of miRNAs essential for plant development? This project will provide knowledge of how a new class of biologically active molecule (micro RNA) regulates expression of genes at sites in the plant that are critical for growth and development. MicroRNAs are believed to influence the size and shape of plants, how rapidly they grow and how well they produce and fill seeds. These molecules are part of a group of bioactive signals that move throughout the plant, functioning like hormones but directly influencing how well critical genes work. Their exploitation holds great promise for manipulating plant performance and enhancing crop yields. Read moreRead less
Coping with flooding: nutrient transport in oxygen-deprived roots. Flooding damages plants by reducing oxygen supply to roots. The project will study effects of low oxygen on nutrient transport by roots. Understanding root functioning during low oxygen will enhance knowledge of plant acclimation to soil water logging. The project will contribute to the National Goal of 'Responding to Climate Change and Variability'.
Carboxylate exudation and phosphate nutrition in Hakea prostrata (Proteaecea). Nonmycorrhizal Proteaceae are very successful in acquiring phosphate from nutrient-impoverished soils; their cluster roots account for this. They are also extremely sensitive to phosphate toxicity. We will elucidate aspects of production and release of carboxylates that are associated with functioning of cluster roots, using Hakea prostrata (Proteaceae) as a model. Types and rates of exudation by cluster roots, as in ....Carboxylate exudation and phosphate nutrition in Hakea prostrata (Proteaecea). Nonmycorrhizal Proteaceae are very successful in acquiring phosphate from nutrient-impoverished soils; their cluster roots account for this. They are also extremely sensitive to phosphate toxicity. We will elucidate aspects of production and release of carboxylates that are associated with functioning of cluster roots, using Hakea prostrata (Proteaceae) as a model. Types and rates of exudation by cluster roots, as influenced by development and environmental signals, will be assessed. Our findings will provide key information on adaptive mechanisms associated with both phosphate acquisition from phosphate-fixing soils and phosphate toxicity.Read moreRead less
Genome Approaches to Investigate Metabolic Coordination in Plant Cells. Metabolism of C and N in legume nodules requires interaction between the symbiotic bacteria and plant organelles, particularly metabolism in plastids and mitochondria. Fixed N is assimilated through the de novo synthesis of purines in both plastids and mitochondria. However, each of the nine pathway enzymes is encoded by a single gene, indicating each protein is targeted to both organelles. Purine metabolism will provide ....Genome Approaches to Investigate Metabolic Coordination in Plant Cells. Metabolism of C and N in legume nodules requires interaction between the symbiotic bacteria and plant organelles, particularly metabolism in plastids and mitochondria. Fixed N is assimilated through the de novo synthesis of purines in both plastids and mitochondria. However, each of the nine pathway enzymes is encoded by a single gene, indicating each protein is targeted to both organelles. Purine metabolism will provide a model to assess the more general occurrence of dual-targeted proteins in plants. The aim is to identify and eventually exploit the signalling mechanism(s) that mediate communication between plastids and mitochondria.Read moreRead less
Protein Complexes and Supercomplexes of Plant Organelles. Ample parts of plant primary metabolism occur in subcellular structures called mitochondria, plastids and peroxisomes. They are vital for plant growth and development and are central to the early success of germinating and growing seedlings. This project intends to analyze the protein complexes and supercomplexes within these organelles using state of the art instrumentation and technologies. Findings from this research have the potential ....Protein Complexes and Supercomplexes of Plant Organelles. Ample parts of plant primary metabolism occur in subcellular structures called mitochondria, plastids and peroxisomes. They are vital for plant growth and development and are central to the early success of germinating and growing seedlings. This project intends to analyze the protein complexes and supercomplexes within these organelles using state of the art instrumentation and technologies. Findings from this research have the potential to directly flow into the plant biotechnology industry and could assist the future development of Australian agriculture through genetic improvements. The expertise developed by this work will ensure that Australia is well placed to meet future needs and to generally improve agricultural technology. Read moreRead less
Functional characterisation of novel transport protein in plants. The products of plants provide the food we eat, the air we breath and it is recognised that they play an integral role in defining and protecting the environment. Thus it is essential to understand how plant work to allow their use in novel applications and to adapt to changing environments. Many aspects of plant metabolism are unique to plants and thus we cannot guess or estimate importance from studies in animals or fungi. Appro ....Functional characterisation of novel transport protein in plants. The products of plants provide the food we eat, the air we breath and it is recognised that they play an integral role in defining and protecting the environment. Thus it is essential to understand how plant work to allow their use in novel applications and to adapt to changing environments. Many aspects of plant metabolism are unique to plants and thus we cannot guess or estimate importance from studies in animals or fungi. Approximately 4,000 proteins are specific to plants, or contain domains that are unique to plants. This investigation proposes to elucidate the function of some of these novel proteins, transporters, that play a critical role in transport processes in cells.Read moreRead less
Functional genomics approaches to the mechanisms of starch mobilisation in Arabidopsis. Starch is a key carbon and energy reserve that underpins plant growth. This in turn underpins much of Australia's $60 billion agriculture industry. Starch also provides most of the calories in the human diet and is a renewable commodity supporting manufacturing industries. Dependence of society on starch will increase as it becomes used more for novel materials and for bio-ethanol production, which in turn w ....Functional genomics approaches to the mechanisms of starch mobilisation in Arabidopsis. Starch is a key carbon and energy reserve that underpins plant growth. This in turn underpins much of Australia's $60 billion agriculture industry. Starch also provides most of the calories in the human diet and is a renewable commodity supporting manufacturing industries. Dependence of society on starch will increase as it becomes used more for novel materials and for bio-ethanol production, which in turn will create new jobs in the rural economy. A major quality problem in cereal grain is pre-harvest starch breakdown caused by warm wet weather triggering events associated with germination. By understanding starch metabolism in plants we will be better able to manage and enhance growth of crop plants, starch production and seed quality.Read moreRead less
Metabolomic and genetic approaches to the discovery of genes that direct carbon partitioning in plants. Plants make starch, sucrose, cell walls (fibre), oil, organic acids, vitamins and other products of great economic and social importance. The partitioning of carbon resources into such products determines crop productivity and quality. This partitioning is strongly influenced by nutrients, water and salinity. The powerful genomics resources of Arabidopsis including the new discipline of metabo ....Metabolomic and genetic approaches to the discovery of genes that direct carbon partitioning in plants. Plants make starch, sucrose, cell walls (fibre), oil, organic acids, vitamins and other products of great economic and social importance. The partitioning of carbon resources into such products determines crop productivity and quality. This partitioning is strongly influenced by nutrients, water and salinity. The powerful genomics resources of Arabidopsis including the new discipline of metabolomics, will be deployed to understand the regulation of carbon partitioning in leaves and to discover genes that direct partitioning. National research capability will be enhanced and new resources will be generated to breed crops with improved yield potential and product quality under varied environmental conditions.Read moreRead less