New Methods for Structural Biology in Solution. This project aims to expand the range of applications of nuclear magnetic resonance (NMR) spectroscopy in pharmaceutical research, where NMR spectroscopy is already used routinely for the identification of chemical compounds that bind to protein targets. The techniques developed aim at providing rapid and broadly applicable tools for 3D structure determinations of chemical compounds bound to their protein target, identification of protein-protein i ....New Methods for Structural Biology in Solution. This project aims to expand the range of applications of nuclear magnetic resonance (NMR) spectroscopy in pharmaceutical research, where NMR spectroscopy is already used routinely for the identification of chemical compounds that bind to protein targets. The techniques developed aim at providing rapid and broadly applicable tools for 3D structure determinations of chemical compounds bound to their protein target, identification of protein-protein interaction sites and characterization of protein motions. The limits of NMR spectroscopy will be pushed to analyse systems of significantly increased molecular weights. The project includes applications to drug targets such as the dengue virus NS2B/NS3 protease.Read moreRead less
New Methods for Structural Biology in Solution. New technologies will be developed that are sufficiently rapid and inexpensive to compete with and replace the mutagenesis experiments that biologists usually perform to identify and characterize the functionally important parts of a protein. Nuclear magnetic resonance (NMR) spectroscopy techniques in combination with various selective labelling schemes will be developed with the goal of identification and structural characterization of protein-lig ....New Methods for Structural Biology in Solution. New technologies will be developed that are sufficiently rapid and inexpensive to compete with and replace the mutagenesis experiments that biologists usually perform to identify and characterize the functionally important parts of a protein. Nuclear magnetic resonance (NMR) spectroscopy techniques in combination with various selective labelling schemes will be developed with the goal of identification and structural characterization of protein-ligand interactions at increased rates and enhanced accuracy. In addition, the three-dimensional structures of proteins and protein domains of biologically important functions and unknown fold will be determined by NMR. The project aims at techniques of direct impact in pharmaceutical industry.Read moreRead less
THE FIRST DEVELOPMENT OF MULTI-DIMENSIONAL SPECTRO-ELECTROCHEMISTRY AND ITS APPLICATION TO CRUCIAL TRANSFORMATIONS IN INORGANIC SYSTEMS. Electrolysis is a very widespread and efficient method of chemical synthesis both in industry and research. However, the link between the control voltage and the resultant current is often complicated and easily misinterpreted. To overcome this fundamental problem, we propose an optical spectro-electrochemistry instrument based on a two-dimensional CCD detectio ....THE FIRST DEVELOPMENT OF MULTI-DIMENSIONAL SPECTRO-ELECTROCHEMISTRY AND ITS APPLICATION TO CRUCIAL TRANSFORMATIONS IN INORGANIC SYSTEMS. Electrolysis is a very widespread and efficient method of chemical synthesis both in industry and research. However, the link between the control voltage and the resultant current is often complicated and easily misinterpreted. To overcome this fundamental problem, we propose an optical spectro-electrochemistry instrument based on a two-dimensional CCD detection array. This radically new approach enables simultaneous spectroscopic and spatial data mapping in the realm adjacent to an electrode surface. Important applications range from unequivocal identification of elusive molecules to monitoring metal corrosion. Our first objective is to elucidate certain crucial transformations of mono- and binuclear heavy metal complexes.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0882289
Funder
Australian Research Council
Funding Amount
$520,000.00
Summary
New generation mass spectrometers for characterisation of molecular shape and size. The ion mobility mass spectrometer (IMMS at UOW) will be the first of its kind in Australia, and together with the ion trap mass spectrometer (ITMS at ANU) will continue the tradition of this partnership in providing researchers with cutting-edge instrumentation for nationally and internationally important projects including: (i) fundamental understanding of the ways in which biomolecules recognize one another, ( ....New generation mass spectrometers for characterisation of molecular shape and size. The ion mobility mass spectrometer (IMMS at UOW) will be the first of its kind in Australia, and together with the ion trap mass spectrometer (ITMS at ANU) will continue the tradition of this partnership in providing researchers with cutting-edge instrumentation for nationally and internationally important projects including: (i) fundamental understanding of the ways in which biomolecules recognize one another, (ii) investigating the structure(s) of lipids (fats) in cardiovascular disease and cataract, (iii) developing anticancer drugs, and (iv) development of new materials.Read moreRead less
Deciphering the early Solar System chronology and planetary chemistry using isotope systematics of meteoritic zircon. This project addresses the early evolution of our Solar System that is one of the most important questions in Earth and Planetary sciences. It will use Australia's meteorites and innovative analytical techniques developed in Australia. High impact scientific results produced in this project will be to the national benefit in terms of international recognition of our unique capabi ....Deciphering the early Solar System chronology and planetary chemistry using isotope systematics of meteoritic zircon. This project addresses the early evolution of our Solar System that is one of the most important questions in Earth and Planetary sciences. It will use Australia's meteorites and innovative analytical techniques developed in Australia. High impact scientific results produced in this project will be to the national benefit in terms of international recognition of our unique capability in this high profile and competitive research field. Furthermore, by providing new constraints on the initial state of geochemical evolution of the terrestrial planets, this work will further our knowledge of the subsequent evolution of the Earth's mantle and crust, leading to better models for Australian continent development and its deep-Earth resources.Read moreRead less
Strategies for the detection of designer steroids in greyhounds. Strategies for the detection of designer steroids in greyhounds. This project aims to develop strategies to detect designer steroids in racing greyhounds to safeguard industry integrity and animal welfare. These strategies include in vivo and comparative in vitro studies of steroid metabolism, mass-spectrometric elucidation of metabolite structures, and synthesis of putative steroid metabolites with different stereochemistries and ....Strategies for the detection of designer steroids in greyhounds. Strategies for the detection of designer steroids in greyhounds. This project aims to develop strategies to detect designer steroids in racing greyhounds to safeguard industry integrity and animal welfare. These strategies include in vivo and comparative in vitro studies of steroid metabolism, mass-spectrometric elucidation of metabolite structures, and synthesis of putative steroid metabolites with different stereochemistries and oxidation patterns as reference materials. This project will use major metabolites identified by these studies to develop analytical procedures to test for drug abuse at greyhound race meetings. The project will target twelve readily available designer steroid supplements that have significant risk of abuse.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0989539
Funder
Australian Research Council
Funding Amount
$250,000.00
Summary
Purchase of a state-of-the-art high resolution inductively coupled plasma mass spectrometer. This new state-of-the-art mass spectrometer with enhanced capability will allow Tasmanian researchers to accurately determine the elemental composition of their samples of interest. The instrument will be extremely sensitive and will be able to detect elements to very low concentrations. It will be used to support a diverse range of local research projects of international significance, for example the ....Purchase of a state-of-the-art high resolution inductively coupled plasma mass spectrometer. This new state-of-the-art mass spectrometer with enhanced capability will allow Tasmanian researchers to accurately determine the elemental composition of their samples of interest. The instrument will be extremely sensitive and will be able to detect elements to very low concentrations. It will be used to support a diverse range of local research projects of international significance, for example the environmental assessment of clean and contaminated sites, chemical synthesis on a miniature scale using micro-chips, and the monitoring of selected elements of key importance for human health.Read moreRead less
DROP DEFORMATION IN CONFINED MICROFLUIDIC GEOMETRIES. Increasingly, high technology applications in biotechnology and microtechnology industries need to process complex (non-Newtonian) fluids with dispersed particles/droplets in channels as small as several microns (microfluidics). A computational fluid dynamic model of non-Newtonian droplet deformation in microfluidic geometries will be developed, and validated using experimental measurements of the flow field in this project. The aim is to und ....DROP DEFORMATION IN CONFINED MICROFLUIDIC GEOMETRIES. Increasingly, high technology applications in biotechnology and microtechnology industries need to process complex (non-Newtonian) fluids with dispersed particles/droplets in channels as small as several microns (microfluidics). A computational fluid dynamic model of non-Newtonian droplet deformation in microfluidic geometries will be developed, and validated using experimental measurements of the flow field in this project. The aim is to understand and quantify factors influencing droplet deformation. Coupling non-Newtonian characteristics with microfluidic geometries will allow the continuous manufacture of micro-particles of specified size and shape for existing and new applications, and will provide guidance for further extending the process to nano-particle manufacture.Read moreRead less