The role of central carbon metabolism in cell cycle control in bacteria. Bacteria are simple organisms, yet we still do not understand how they coordinate their growth with their reproduction so faithfully, generation after generation, to produce viable newborn cells. The new discovery of a link between the food bacteria eat and the first stage of their cell division now provides the opportunity to elucidate how bacteria 'measure' their energy production to control their proliferation. This proj ....The role of central carbon metabolism in cell cycle control in bacteria. Bacteria are simple organisms, yet we still do not understand how they coordinate their growth with their reproduction so faithfully, generation after generation, to produce viable newborn cells. The new discovery of a link between the food bacteria eat and the first stage of their cell division now provides the opportunity to elucidate how bacteria 'measure' their energy production to control their proliferation. This project combines the latest technology with complementary expertise in bacterial cell division and metabolism. This should identify the mechanism that integrates these fundamental pathways in bacteria, crucial to both their survival and ability to cause infection.Read moreRead less
Identifying how bacterial cells find their middle: a new perspective. This project will reveal new information about how bacterial cells divide with high precision to ensure that each newborn cell contains the correct genetic material. The research uses frontier techniques, provides innovative training to young Australian researchers, and will identify new ways to treat infections caused by bacteria.
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE160100127
Funder
Australian Research Council
Funding Amount
$355,000.00
Summary
Superresolution fluorescence imaging in microbiology. Superresolution fluorescence imaging in microbiology:
This project involves the purchase of new, and upgrade of existing, fluorescence imaging tools to facilitate the study of intracellular processes in microbial systems at significantly higher spatial and temporal resolutions than hitherto possible. Visualisation of the structure and dynamics of intracellular molecular assemblies at maximal resolution is required to understand protein funct ....Superresolution fluorescence imaging in microbiology. Superresolution fluorescence imaging in microbiology:
This project involves the purchase of new, and upgrade of existing, fluorescence imaging tools to facilitate the study of intracellular processes in microbial systems at significantly higher spatial and temporal resolutions than hitherto possible. Visualisation of the structure and dynamics of intracellular molecular assemblies at maximal resolution is required to understand protein function inside living cells. The new equipment is designed to provide a fast super-resolution imaging system to study the intracellular dynamics of proteins in vitro and a super-resolution microscope to visualise structures and assemblies inside microbes with a resolution of tens of nanometres, putting in vitro biochemistry into the context of a living cell. Read moreRead less
The control of archaeal cell structure by tubulin-family proteins. The objective of this project is to deliver new insights into the evolution and diversity of cell structure and function. Cell theory has been a cornerstone of biology for over 150 years. Yet how early cells developed into modern forms is still a mystery. The primitive and poorly understood third domain of life, Archaea, could hold clues. Recently, proteins were discovered in archaea that are related to the tubulin proteins of al ....The control of archaeal cell structure by tubulin-family proteins. The objective of this project is to deliver new insights into the evolution and diversity of cell structure and function. Cell theory has been a cornerstone of biology for over 150 years. Yet how early cells developed into modern forms is still a mystery. The primitive and poorly understood third domain of life, Archaea, could hold clues. Recently, proteins were discovered in archaea that are related to the tubulin proteins of all higher organisms, which provide the structural framework of cells essential for survival. This project aims to reveal the basis of how the archaeal tubulin proteins control cell shape in response to environmental change, and to develop a new paradigm for archaeal cell biology. This may find application in Australia's biotechnology industries.Read moreRead less
An interdisciplinary approach to host-pathogen interactions in infection. This project aims to understand the molecular and cellular interactions between host and parasite, as well as providing a quantitative framework for analysing infection dynamics in other systems. Infection involves a complex interaction between the host and the parasite, which is very dynamic and therefore difficult to study by traditional sampling and analysis approaches. This project has combined mathematical modelling w ....An interdisciplinary approach to host-pathogen interactions in infection. This project aims to understand the molecular and cellular interactions between host and parasite, as well as providing a quantitative framework for analysing infection dynamics in other systems. Infection involves a complex interaction between the host and the parasite, which is very dynamic and therefore difficult to study by traditional sampling and analysis approaches. This project has combined mathematical modelling with a novel experimental protocol to allow the study of kinetics of parasite replication in vivo. Expected outcomes will provide significant benefits, such as new avenues for vaccination and immune intervention.Read moreRead less
Understanding the dynamics of malaria infection. Malaria infection kills around one million patients each year and this project involves an interdisciplinary team who will directly measure how the parasite grows and is killed by the immune system. A better understanding of parasite growth and control will help develop better drugs therapy and vaccination for this important infection.
Discovery Early Career Researcher Award - Grant ID: DE160100615
Funder
Australian Research Council
Funding Amount
$348,200.00
Summary
Harnessing chain-forming diatoms for improved lipid biofuel production. The aim of this project is to unlock the molecular secrets of highly productive chain-forming diatom microalgae that allow them to produce high levels of biofuel lipids. The formation of multicellular chains appears key to the success of some of the most widespread and productive diatom species. Through a combination of systems biology, bioinformatics, and genetics experiments, this project aims to investigate the relationsh ....Harnessing chain-forming diatoms for improved lipid biofuel production. The aim of this project is to unlock the molecular secrets of highly productive chain-forming diatom microalgae that allow them to produce high levels of biofuel lipids. The formation of multicellular chains appears key to the success of some of the most widespread and productive diatom species. Through a combination of systems biology, bioinformatics, and genetics experiments, this project aims to investigate the relationship between chain formation and biofuel lipid productivity in Chaetoceros diatoms, and to discover genes and molecules that encode and influence these traits. The knowledge and technology generated as a result may improve biofuel yields, increase the robustness of species growing in open pond systems, and reduce processing costs such as de-watering.Read moreRead less
The Great Escape: Mechanisms for dispersal of microbial communities from surfaces. Bacteria respond to a variety of environmental cues, including nutrient concentration, to optimise their growth strategy. One key growth strategy is the formation of biofilms or surface associated microbial communities. The aim of this project is to determine the molecular pathway for cAMP mediated starvation induced dispersal of bacterial biofilms. Our preliminary data suggest that the cAMP pathway overlaps with ....The Great Escape: Mechanisms for dispersal of microbial communities from surfaces. Bacteria respond to a variety of environmental cues, including nutrient concentration, to optimise their growth strategy. One key growth strategy is the formation of biofilms or surface associated microbial communities. The aim of this project is to determine the molecular pathway for cAMP mediated starvation induced dispersal of bacterial biofilms. Our preliminary data suggest that the cAMP pathway overlaps with other intracellular second messengers, such as c-di-GMP, to control dispersal. Further, these second messengers may act at the level of subcellular pools that interact with closely associated protein complexes to control complex behaviours such as biofilm formation and dispersal.Read moreRead less
A new molecular machine required for bacterial development into spores. This project aims to provide new knowledge on how bacteria produce dormant, stress-resistant cells called spores, and how bacteria transport molecules across their cellular layers to execute biological functions. Spores can act as a source of new and recurring infections in many bacterial pathogens. This project expects to reveal molecular details on a new class of nanomachines required for spore development. The new knowled ....A new molecular machine required for bacterial development into spores. This project aims to provide new knowledge on how bacteria produce dormant, stress-resistant cells called spores, and how bacteria transport molecules across their cellular layers to execute biological functions. Spores can act as a source of new and recurring infections in many bacterial pathogens. This project expects to reveal molecular details on a new class of nanomachines required for spore development. The new knowledge generated may expand the arsenal of molecular targets required to develop strategies interfering with spore formation. This provides a platform from which industry could attract investment for exploring innovative strategies for controlling bacteria.Read moreRead less
Microbial sulphatises in the rhizosphere and their control by interactions with plants. Plant-microbe interactions are critical in mobilizing soil sulphur for crop growth. This project will identify the microbes responsible for delivering sulphur to two major Australian crops, and will examine how the plants stimulate this activity in their root zone. The results have potential application for sustainable agriculture in Australia.