The basis of recognition and disposal of dysfunctional proteins by clusterin. When proteins become damaged they can precipitate. A blood protein called clusterin prevents precipitation of damaged proteins. Clusterin does this by forming complexes with the damaged proteins. Clusterin is the first blood protein known to do this. We will discover which parts of clusterin are responsible for this activity. We will also discover whether cells can take up and dispose of the complexes of clusterin and ....The basis of recognition and disposal of dysfunctional proteins by clusterin. When proteins become damaged they can precipitate. A blood protein called clusterin prevents precipitation of damaged proteins. Clusterin does this by forming complexes with the damaged proteins. Clusterin is the first blood protein known to do this. We will discover which parts of clusterin are responsible for this activity. We will also discover whether cells can take up and dispose of the complexes of clusterin and damaged proteins. This work is important because some diseases (eg, Alzheimers disease) involve the toxic effects of abnormal protein precipitation. Understanding how clusterin works may help in developing better treatments for these diseases.Read moreRead less
Novel Mass Spectrometric Approaches to the Study of Protein-Protein Interactions. Protein-protein interactions mediate all fundamental cellular processes, yet the structural complexity of protein assemblies mean they are often difficult to characterise using traditional analytical methods. This project will develop and demonstrate novel mass spectrometric approaches towards a molecular level description of the structure and interactions of biological protein complexes, which in turn may underpi ....Novel Mass Spectrometric Approaches to the Study of Protein-Protein Interactions. Protein-protein interactions mediate all fundamental cellular processes, yet the structural complexity of protein assemblies mean they are often difficult to characterise using traditional analytical methods. This project will develop and demonstrate novel mass spectrometric approaches towards a molecular level description of the structure and interactions of biological protein complexes, which in turn may underpin the rational design of drugs for the treatment of a range of human health conditions. This project will also provide training of young researchers to the highest international standards in mass spectrometry and protein science, for benefit to Australian industry and research.Read moreRead less
Negative ion mass spectrometry: fundamentals and applied applications. 1. We seek to establish negative ion mass spectrometry as a major technological tool for the sequencing of peptides and proteins. In this context, we will investigate the structures and modes of action of peptide complexes which may be of importance for the treatment and control of heart disease and stroke.
2. Negative ions of known structure will be converted (in the mass spectrometer) into transient (and reactive) molecule ....Negative ion mass spectrometry: fundamentals and applied applications. 1. We seek to establish negative ion mass spectrometry as a major technological tool for the sequencing of peptides and proteins. In this context, we will investigate the structures and modes of action of peptide complexes which may be of importance for the treatment and control of heart disease and stroke.
2. Negative ions of known structure will be converted (in the mass spectrometer) into transient (and reactive) molecules which are present in interstellar ice and dust clouds. The structures and chemistry of such molecules are of importance in understanding the origins of life on this planet.Read moreRead less
Functional characterisation of poly-histidine triad proteins. This project aims to understand the role and function of a novel family of surface proteins produced by Streptococci. These so-called polyhistidine triad proteins are known to contribute to capacity to cause disease in animals and humans, but we need to know how they work, as they may be excellent targets for novel drugs or vaccines.
Novel perspectives on the function of AB5 toxin B subunits in pathogenic bacterial. AB5 toxins are produced by bacteria that cause important diseases in humans and livestock. This project tests the hypothesis that the components of the toxins responsible for binding to host cells and tissues also directly contribute to cellular damage, thereby providing a better understanding of how AB5 toxin-producing bacteria cause disease.
Negative ion mass spectrometry: fundamental studies and applied applications. We seek to:
(i) establish negative ion mass spectrometry as an analytical tool for the sequencing of proteins and to probe the structures of active peptide Ca2+ calmodulin complexes which, for example, inhibit the formation of NO from nitric oxide synthases. Controlling the concentration of nitric oxide may assist with the treatment of inflammatory and cardiac diseases, stroke and diseases of the central nervous syst ....Negative ion mass spectrometry: fundamental studies and applied applications. We seek to:
(i) establish negative ion mass spectrometry as an analytical tool for the sequencing of proteins and to probe the structures of active peptide Ca2+ calmodulin complexes which, for example, inhibit the formation of NO from nitric oxide synthases. Controlling the concentration of nitric oxide may assist with the treatment of inflammatory and cardiac diseases, stroke and diseases of the central nervous system.
(ii) to study the possibility of the formation of biological molecules of life in interstellar regions
(iii) to train graduate students to international standards in the chemistry of proteomics.Read moreRead less
Cyberkids and cyberworlds: new literacies, identities and communities in formation. Little is known of the online cyberworlds of young Australians. Yet they are the highest users of Information and Communications Technologies (ICTs). This project theorises that these cyberworlds are sites for the development of new forms of literacy, identity and community. This study will achieve a new perspective by utilising a multi-disciplinary approach to produce case studies that examine new literacies an ....Cyberkids and cyberworlds: new literacies, identities and communities in formation. Little is known of the online cyberworlds of young Australians. Yet they are the highest users of Information and Communications Technologies (ICTs). This project theorises that these cyberworlds are sites for the development of new forms of literacy, identity and community. This study will achieve a new perspective by utilising a multi-disciplinary approach to produce case studies that examine new literacies and communities generated online and their consequences for literacy and ICT education practice. Such information is vital in Australia and internationally if education is to produce the highly technologically literate citizens on whom future economic prosperity depends.Read moreRead less
Teaching reading in Australia: An historical investigation of early reading pedagogy, the figure of the teacher and literacy education. This project will make a distinctive national and international contribution to reading pedagogy and literacy research by reconceptualising debates about the teacher of reading and establishing an historical foundation for the study of reading pedagogy in teacher education and professional development practices. Teacher education, already a site of national conc ....Teaching reading in Australia: An historical investigation of early reading pedagogy, the figure of the teacher and literacy education. This project will make a distinctive national and international contribution to reading pedagogy and literacy research by reconceptualising debates about the teacher of reading and establishing an historical foundation for the study of reading pedagogy in teacher education and professional development practices. Teacher education, already a site of national concern, will be reinvigorated by new insights and frameworks for training through access by teacher educators to the corpus of data on a website and through publications and conference presentations that will provide a new vision of the complex processes involved in teaching reading.Read moreRead less
On Becoming a Middle School Teacher: Reclaiming the Wasteland of the Middle Years of Schooling. This project aims to investigate how teachers in Australian government schools committed to the middle years of schooling (ages 14-16), are 'reinventing' their teaching in the current policy reform context. The research will explore how policies support or hinder teachers in establishing and sustaining educative relationships with young adolescents. Detailed ethnographic case studies will examine ho ....On Becoming a Middle School Teacher: Reclaiming the Wasteland of the Middle Years of Schooling. This project aims to investigate how teachers in Australian government schools committed to the middle years of schooling (ages 14-16), are 'reinventing' their teaching in the current policy reform context. The research will explore how policies support or hinder teachers in establishing and sustaining educative relationships with young adolescents. Detailed ethnographic case studies will examine how teachers restructure and reculture what schools do to better serve students who are increasingly 'at risk'. The project will provide crucial understandings into the theory, policy and practice of teaching necessary to enable all young adolescents to experience a rewarding high school education.Read moreRead less
Mandated literacy assessment and the reorganisation of teachers' work. The study will inform practitioners, teacher educators and educational policy-makers about the ways that teachers' work is being changed by the introduction of mandated standardised assessment and reporting processes. The research will provide insights into the ways in which teachers need to adapt standardised processes and policies to account for the varied student and community populations they serve. This is significant fo ....Mandated literacy assessment and the reorganisation of teachers' work. The study will inform practitioners, teacher educators and educational policy-makers about the ways that teachers' work is being changed by the introduction of mandated standardised assessment and reporting processes. The research will provide insights into the ways in which teachers need to adapt standardised processes and policies to account for the varied student and community populations they serve. This is significant for educational policy as recent international studies of students' literacy performance suggest Australia is lagging in terms of equity for low SES students. Read moreRead less