The basis of recognition and disposal of dysfunctional proteins by clusterin. When proteins become damaged they can precipitate. A blood protein called clusterin prevents precipitation of damaged proteins. Clusterin does this by forming complexes with the damaged proteins. Clusterin is the first blood protein known to do this. We will discover which parts of clusterin are responsible for this activity. We will also discover whether cells can take up and dispose of the complexes of clusterin and ....The basis of recognition and disposal of dysfunctional proteins by clusterin. When proteins become damaged they can precipitate. A blood protein called clusterin prevents precipitation of damaged proteins. Clusterin does this by forming complexes with the damaged proteins. Clusterin is the first blood protein known to do this. We will discover which parts of clusterin are responsible for this activity. We will also discover whether cells can take up and dispose of the complexes of clusterin and damaged proteins. This work is important because some diseases (eg, Alzheimers disease) involve the toxic effects of abnormal protein precipitation. Understanding how clusterin works may help in developing better treatments for these diseases.Read moreRead less
Novel Mass Spectrometric Approaches to the Study of Protein-Protein Interactions. Protein-protein interactions mediate all fundamental cellular processes, yet the structural complexity of protein assemblies mean they are often difficult to characterise using traditional analytical methods. This project will develop and demonstrate novel mass spectrometric approaches towards a molecular level description of the structure and interactions of biological protein complexes, which in turn may underpi ....Novel Mass Spectrometric Approaches to the Study of Protein-Protein Interactions. Protein-protein interactions mediate all fundamental cellular processes, yet the structural complexity of protein assemblies mean they are often difficult to characterise using traditional analytical methods. This project will develop and demonstrate novel mass spectrometric approaches towards a molecular level description of the structure and interactions of biological protein complexes, which in turn may underpin the rational design of drugs for the treatment of a range of human health conditions. This project will also provide training of young researchers to the highest international standards in mass spectrometry and protein science, for benefit to Australian industry and research.Read moreRead less
Nanosized peptide nucleic acid - metal complex hybrids as catalysts for the cleavage of phosphate ester bonds in biological molecules. The information from Human Genome Project is being used to generate molecules with a variety of therapeutic and diagnostic applications. The capability to design, synthesise and manipulate functional molecules that mimic biological processes will underpin many emerging applications. In this project, macrocyclic metal complexes that catalyse the cleavage of phosph ....Nanosized peptide nucleic acid - metal complex hybrids as catalysts for the cleavage of phosphate ester bonds in biological molecules. The information from Human Genome Project is being used to generate molecules with a variety of therapeutic and diagnostic applications. The capability to design, synthesise and manipulate functional molecules that mimic biological processes will underpin many emerging applications. In this project, macrocyclic metal complexes that catalyse the cleavage of phosphate ester bonds in biological molecules will be developed. Active complexes will be incorporated into nanosized peptide nucleic acid (PNA) - metal complex hybrids and applied as artificial enzymes in the sequence specific cleavage of RNA and DNA. Novel applications of these ?artificial enzymes? in biotechnology are anticipated.Read moreRead less
Natural Product-derived Proteomics Probes for Specific Detection of Protein Kinase C Activities. Better health care depends on how well diseases are understood and how accurately disease analysis and diagnosis can be carried out. This is not only important to disease treatment but also prevention. This project will first generate new compounds that could have improved therapeutic value in cancer treatment. In addition, these compounds will be further engineered to provide a new technology of ....Natural Product-derived Proteomics Probes for Specific Detection of Protein Kinase C Activities. Better health care depends on how well diseases are understood and how accurately disease analysis and diagnosis can be carried out. This is not only important to disease treatment but also prevention. This project will first generate new compounds that could have improved therapeutic value in cancer treatment. In addition, these compounds will be further engineered to provide a new technology of tracing the molecular signature of diseases such as cancer for early detection and better preventative care and treatment. This will create new economic advantage and contributes to the transformation of Australian health care industry.Read moreRead less
Negative ion mass spectrometry: fundamentals and applied applications. 1. We seek to establish negative ion mass spectrometry as a major technological tool for the sequencing of peptides and proteins. In this context, we will investigate the structures and modes of action of peptide complexes which may be of importance for the treatment and control of heart disease and stroke.
2. Negative ions of known structure will be converted (in the mass spectrometer) into transient (and reactive) molecule ....Negative ion mass spectrometry: fundamentals and applied applications. 1. We seek to establish negative ion mass spectrometry as a major technological tool for the sequencing of peptides and proteins. In this context, we will investigate the structures and modes of action of peptide complexes which may be of importance for the treatment and control of heart disease and stroke.
2. Negative ions of known structure will be converted (in the mass spectrometer) into transient (and reactive) molecules which are present in interstellar ice and dust clouds. The structures and chemistry of such molecules are of importance in understanding the origins of life on this planet.Read moreRead less
Knowledge to enhance Science Teaching and Learning: Pursuing scholarship through explicitly enacting expert science teachers' pedagogical content knowledge. This research will enhance understandings of science teaching and learning allowing science teachers to share their knowledge in meaningful ways. In teacher preparation this will be particularly valuable by 'fast-tracking' learning about teaching science and minimize the process of the next generation of teachers feeling as though they are r ....Knowledge to enhance Science Teaching and Learning: Pursuing scholarship through explicitly enacting expert science teachers' pedagogical content knowledge. This research will enhance understandings of science teaching and learning allowing science teachers to share their knowledge in meaningful ways. In teacher preparation this will be particularly valuable by 'fast-tracking' learning about teaching science and minimize the process of the next generation of teachers feeling as though they are rediscovering that which is already known by experienced teachers of science. In articulating science teachers' knowledge, enhanced valuing their knowledge and skills of practice will occur. Such outcomes will place understanding of science teaching and learning at the forefront of teacher professionalism and positively impact work on professional standards in teaching.Read moreRead less
The role of subject-matter knowledge in teaching science and mathematics: Practices of teachers with advanced qualifications in the sciences. High quality science and mathematics education is central to economic prosperity. This study will achieve two outcomes. Firstly it explores the importance of subject matter knowledge in teaching. Much has been studied about the knowledge of teachers but limited research has been conducted with teachers who have substantial subject-matter knowledge. These f ....The role of subject-matter knowledge in teaching science and mathematics: Practices of teachers with advanced qualifications in the sciences. High quality science and mathematics education is central to economic prosperity. This study will achieve two outcomes. Firstly it explores the importance of subject matter knowledge in teaching. Much has been studied about the knowledge of teachers but limited research has been conducted with teachers who have substantial subject-matter knowledge. These findings will inform the current debate about the appropriate models of teacher education and the balance of emphasis on content knowledge and pedagogical knowledge. Secondly, given a number of attempts to attract mid career professionals with high qualifications to teaching, the study will provide evidence on how these people transition to their new careers and what support is needed.Read moreRead less
Comparative effectiveness of three literacy teaching approaches for young children with reading difficulties. This project addresses concerns expressed by the Minister for Education, Science and Training, and the Australian Senate in regard to the relative poor performance of Australian children in national reading tests. Specifically, the project seeks to identify the most effective early literacy instructional method or methods to redress the growing concern that Australian children are droppi ....Comparative effectiveness of three literacy teaching approaches for young children with reading difficulties. This project addresses concerns expressed by the Minister for Education, Science and Training, and the Australian Senate in regard to the relative poor performance of Australian children in national reading tests. Specifically, the project seeks to identify the most effective early literacy instructional method or methods to redress the growing concern that Australian children are dropping behind in international competitiveness in reading and literacy achievement. The study concentrates on early reading development and to inform on teacher education and classroom teaching practices. Read moreRead less
Research capacity-building: the development of Australian PhD programs in national and emerging global contexts. PhD programs in universities are the principal means by which new researchers are prepared for work in the knowledge economy. This project develops a comprehensive coded database of every PhD awarded in Australia since the first programs in the 1940s. It the will study fourteen different PhD programs that have developed during this period in four universities. The project focuses on ....Research capacity-building: the development of Australian PhD programs in national and emerging global contexts. PhD programs in universities are the principal means by which new researchers are prepared for work in the knowledge economy. This project develops a comprehensive coded database of every PhD awarded in Australia since the first programs in the 1940s. It the will study fourteen different PhD programs that have developed during this period in four universities. The project focuses on how these programs develop research capacity within disciplines and how these are related to the changing social, cultural and economic needs of Australia. The project will assist in planning future PhD programs and research capacity development.Read moreRead less
Australian doctoral graduates' publication, professional and community outcomes. The project produces benefits to government, universities, Deans and Directors of Graduate Studies and those with a scholarly interest in doctoral education. It identifies, quantifies and explains the relationship between PhD graduates' thesis outcomes (especially, publications) and their professional and community outcomes up to ten years from graduation. The findings will be disseminated as universities, governmen ....Australian doctoral graduates' publication, professional and community outcomes. The project produces benefits to government, universities, Deans and Directors of Graduate Studies and those with a scholarly interest in doctoral education. It identifies, quantifies and explains the relationship between PhD graduates' thesis outcomes (especially, publications) and their professional and community outcomes up to ten years from graduation. The findings will be disseminated as universities, government and academies debate the RQF outcomes and whether the quality and impact of doctorates be included in the next RQF. The findings enable universities to enhance policy and practice to ensure worthwhile outcomes from doctoral programs and graduates. The project advances scholarship about doctorates and research education.Read moreRead less