Proteomic and Transcriptional Profiling of Cartilage. Gene expression and signalling pathways that regulate cartilage formation, and its orderly transition to bone, are poorly described. Our studies will, for the first time, combine two complementary cutting-edge approaches, protein identification by proteomic analysis, and mRNA profiling by microarray analysis, to define these pathways and develop a comprehensive catalogue of proteins and gene expression patterns during cartilage development a ....Proteomic and Transcriptional Profiling of Cartilage. Gene expression and signalling pathways that regulate cartilage formation, and its orderly transition to bone, are poorly described. Our studies will, for the first time, combine two complementary cutting-edge approaches, protein identification by proteomic analysis, and mRNA profiling by microarray analysis, to define these pathways and develop a comprehensive catalogue of proteins and gene expression patterns during cartilage development and bone formation. This information will provide insight into the regulation of cartilage differentiation, maturation and structure, and will provide a critical platform for the development of more sophisticated cartilage and bone biomaterials for improved tissue repair and regeneration.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE100100010
Funder
Australian Research Council
Funding Amount
$720,000.00
Summary
A 5-D Correlative Imaging Platform: Combining the strengths of light and electron microscopy. This will be Australia's first dedicated five-dimensional multiphoton-microscopy platform, allowing observation of dynamic structures across different length and time scales under controlled temperatures, followed by high-resolution electron microscopy studies on the same samples. This platform will provide a unique characterisation tool to Australia's top-flight investigators, and so contribute to the ....A 5-D Correlative Imaging Platform: Combining the strengths of light and electron microscopy. This will be Australia's first dedicated five-dimensional multiphoton-microscopy platform, allowing observation of dynamic structures across different length and time scales under controlled temperatures, followed by high-resolution electron microscopy studies on the same samples. This platform will provide a unique characterisation tool to Australia's top-flight investigators, and so contribute to the nation's research priorities. It will enable: fundamental studies of cancer, neural diseases and immune disorders; the development of frontier technologies, such as smart nanomaterials, biosensors and targeted drug delivery; and applied research to help plants and soils adapt to climate variability, and to increase sustainable use of water.Read moreRead less