Metagenomics and the genetic basis of ecology and evolution of communities - complex microbial communities in industrial processes as excellent paradigms. Benefits accrue on two fronts: the international reputation of Australian science in contributing significantly to two new, challenging and highly topical questions in ecology and evolution, and in pioneering a route to better efficiency and control of an important industrial process. Wastewater is rich in organic phosphorus that is damaging t ....Metagenomics and the genetic basis of ecology and evolution of communities - complex microbial communities in industrial processes as excellent paradigms. Benefits accrue on two fronts: the international reputation of Australian science in contributing significantly to two new, challenging and highly topical questions in ecology and evolution, and in pioneering a route to better efficiency and control of an important industrial process. Wastewater is rich in organic phosphorus that is damaging to the environment if untreated, but current understanding enables only rudimentary control of the microbial communities that are the basis of the treatment process. The work will provide unprecedented insight into the mechanisms underpinning the dynamics of phosphorus absorbing microbes in industrial facilities by integrating from gene-to-ecosystem.Read moreRead less
Development and implementation of biodiversity information for sustainable management of South Australian groundwater. Clean potable water is one of the most important resources for human health and a successful economy. Increasingly, subterranean aquifers are used for storage and recovery of water. These aquifers contain dynamic ecosystems, but little is known about species composition or about the importance of the presence of various species for water quality. We will use the latest laborator ....Development and implementation of biodiversity information for sustainable management of South Australian groundwater. Clean potable water is one of the most important resources for human health and a successful economy. Increasingly, subterranean aquifers are used for storage and recovery of water. These aquifers contain dynamic ecosystems, but little is known about species composition or about the importance of the presence of various species for water quality. We will use the latest laboratory techniques and DNA identification methods to provide a template for determining ground water diversity and food web dynamics throughout Australia. This project will lead to a better understanding of how to manage ground water in a sustainable manner.Read moreRead less
EXTRACELLULAR ELECTRON TRANSFER IN BIO-ELECTROCHEMICAL SYSTEMS. Water quality and supply are critical issues in Australia. This project investigates the role of bacteria in maintaining a good freshwater quality, and the influence of environmental parameters on this. It will enable us to assess the role of bacteria on greenhouse gas emissions in a variety of environments. As a result, processes can be developed to alleviate high emissions while simultaneously producing green energy. The proteomic ....EXTRACELLULAR ELECTRON TRANSFER IN BIO-ELECTROCHEMICAL SYSTEMS. Water quality and supply are critical issues in Australia. This project investigates the role of bacteria in maintaining a good freshwater quality, and the influence of environmental parameters on this. It will enable us to assess the role of bacteria on greenhouse gas emissions in a variety of environments. As a result, processes can be developed to alleviate high emissions while simultaneously producing green energy. The proteomics study will deliver, aside from knowledge, redox proteins which find their way to diagnostics and fuel cells. This project substantiates Australia based research at the forefront and enables international anchoring of our expertise.Read moreRead less
Microbial Ecology and Control of Foaming in Anaerobic Digesters. One of the world's most common treatments of biosolids (product of wastewater treatment), anaerobic digestion often suffers from accumulation of biological foam. This foam hinders treatment, personnel health and safety, legal requirements for environmental protection are jeopardised and attempts to control digester foaming are costly. There is a clear lack of knowledge about the organisms involved and causes, therefore no informe ....Microbial Ecology and Control of Foaming in Anaerobic Digesters. One of the world's most common treatments of biosolids (product of wastewater treatment), anaerobic digestion often suffers from accumulation of biological foam. This foam hinders treatment, personnel health and safety, legal requirements for environmental protection are jeopardised and attempts to control digester foaming are costly. There is a clear lack of knowledge about the organisms involved and causes, therefore no informed solutions exist. Molecular DNA techniques, 16SrDNA sequencing and DGGE, will assist in deciphering causes and organisms involved. Research outcomes will present environmental, legal and economical acceptable control strategies for digester foaming to the waste management and water industries.Read moreRead less
Community-level selection: Empirical tests in a microbial system. Given the profile of the question of community-level selection as a long-running controversy, the main benefit of the proposed work, which will critically test the idea in an empirical system, will be to increase recognition of Australia's position as a research nation in evolutionary biology. In exploring mechanisms of floc formation, a key component of wastewater treatment, the work will establish important foundations for impro ....Community-level selection: Empirical tests in a microbial system. Given the profile of the question of community-level selection as a long-running controversy, the main benefit of the proposed work, which will critically test the idea in an empirical system, will be to increase recognition of Australia's position as a research nation in evolutionary biology. In exploring mechanisms of floc formation, a key component of wastewater treatment, the work will establish important foundations for improving the efficiency of wastewater treatment. Improvement in performance of only a few percent will bring important economic savings. This is evidenced by recent commitment of >$US 230 billion to improving the efficiency of wastewater treatment in Germany, Italy and Spain over 5 years.Read moreRead less
Landfill Based Rapid Anaerobic Digestion of Municipal Solid Waste. The rapid digestion of municipal solid waste has the potential to make putrescible landfills obsolete. Waste can be digested prior to landfilling, eliminating odour and the emission of methane. Pre-digestion is currently performed in highly mechanised in-vessel digesters in some European locations. Demonstations in our laboratory have shown similar digestion rates can be achieved in static beds. This technology can be feasibl ....Landfill Based Rapid Anaerobic Digestion of Municipal Solid Waste. The rapid digestion of municipal solid waste has the potential to make putrescible landfills obsolete. Waste can be digested prior to landfilling, eliminating odour and the emission of methane. Pre-digestion is currently performed in highly mechanised in-vessel digesters in some European locations. Demonstations in our laboratory have shown similar digestion rates can be achieved in static beds. This technology can be feasibly scaled to digest waste streams of the size produced by Australian cities. The project will scale up this technology in a series of test cell trials at the Thiess Swanbank landfill near Ipswich, Queensland.Read moreRead less
Interspecies electron transfer in biotechnology. While the project is fundamental in nature, it has direct technological gains to a wide range of biotechnology processes, and in particular, mixed culture anaerobic biotechnology. It therefore promotes Australian bioenergy, biofuel, and commodity renewable chemicals industries, and contributes to the national research priority of an Environmentally Sustainable Australia. In addition, this is a fast moving, high impact area that will demonstrate e ....Interspecies electron transfer in biotechnology. While the project is fundamental in nature, it has direct technological gains to a wide range of biotechnology processes, and in particular, mixed culture anaerobic biotechnology. It therefore promotes Australian bioenergy, biofuel, and commodity renewable chemicals industries, and contributes to the national research priority of an Environmentally Sustainable Australia. In addition, this is a fast moving, high impact area that will demonstrate excellence in Australian research on an international scale. The exciting multidisciplinary nature of the project, excellent management team, and high-class partners will also provide for an excellent experience for the three PhD candidates to be educated through the project.Read moreRead less
Fundamental Knowledge Generation of Organic Solid Waste-Microorganism Interactions. The management of organic solid waste is a major global environmental issue, exacerbated by increasing populations and urbanisation. However, there are biological processes (e.g. anaerobic composting) that show excellent potential as the solution to this global problem. We know that the rate-limiting step to biological organic solid waste degradation is the initial hydrolysis process carried out by microorganisms ....Fundamental Knowledge Generation of Organic Solid Waste-Microorganism Interactions. The management of organic solid waste is a major global environmental issue, exacerbated by increasing populations and urbanisation. However, there are biological processes (e.g. anaerobic composting) that show excellent potential as the solution to this global problem. We know that the rate-limiting step to biological organic solid waste degradation is the initial hydrolysis process carried out by microorganisms. We will study at a microscale the "organic solid waste surface-microorganism" interactions and compare data from digesters with the rumen which is a highly efficient model solid substrate digestion system. Our generated knowledge will be used in future practical applications.Read moreRead less
Functional Analyses of Bacteria Involved in Enhanced Biological Phosphorus Removal from Wastewater. The abundant growth of blue-green algae in global waterways is substantially caused by phosphorus (P) release from wastewater treatment plants. This environmental drama can be mitigated against by P-accumulating bacteria partitioning the P inside their cells. The P-removal process often fails, but since the metabolism of P-accumulating bacteria is unknown, remedial actions are based on conjecture ....Functional Analyses of Bacteria Involved in Enhanced Biological Phosphorus Removal from Wastewater. The abundant growth of blue-green algae in global waterways is substantially caused by phosphorus (P) release from wastewater treatment plants. This environmental drama can be mitigated against by P-accumulating bacteria partitioning the P inside their cells. The P-removal process often fails, but since the metabolism of P-accumulating bacteria is unknown, remedial actions are based on conjecture. This fundamental, knowledge-generating project will address this shortfall and will develop methods to evaluate the activity of P-accumulating bacteria, contributing substantial understanding of their metabolism. The final goal is to promote stable, reproducible P-removal from wastewater.Read moreRead less
The only constant is change: ecology and evolution of phage-host interactions in a model ecosystem. Microorganisms underpin life on Earth, but our understanding of their diversity and activity is limited by our inability to grow most of them in the laboratory. Recently, new techniques have emerged that allow access to the genetic information of all microorganisms by directly sequencing DNA and RNA from the environment. In this research we will further develop these frontier technologies, promoti ....The only constant is change: ecology and evolution of phage-host interactions in a model ecosystem. Microorganisms underpin life on Earth, but our understanding of their diversity and activity is limited by our inability to grow most of them in the laboratory. Recently, new techniques have emerged that allow access to the genetic information of all microorganisms by directly sequencing DNA and RNA from the environment. In this research we will further develop these frontier technologies, promoting this new area of science in Australia. We will apply these techniques to microbial communities involved in wastewater treatment in order to understand the interactions between microorganisms and the viruses that infect them. Understanding this interaction will have important implications for optimising these treatment processes.Read moreRead less