Visualising neuron-glia interactions in the injured central nervous system. The adult brain and spinal cord recovery poorly from injury. Attempts to overcome this problem include methods to promote the intrinsic regenerative capacity of injured neurons, and modulating the inhibitory extracellular environment to become permissive to regeneration. The goal of this project is to investigate an endogenous regenerative mechanism in the injured brain. This project will use the latest, cutting-edge mic ....Visualising neuron-glia interactions in the injured central nervous system. The adult brain and spinal cord recovery poorly from injury. Attempts to overcome this problem include methods to promote the intrinsic regenerative capacity of injured neurons, and modulating the inhibitory extracellular environment to become permissive to regeneration. The goal of this project is to investigate an endogenous regenerative mechanism in the injured brain. This project will use the latest, cutting-edge microscopy techniques to visualise whether the endogenous astrocyte protein metallothionein can promote regeneration in the injured nervous system of living zebrafish. The successful outcomes of this project will provide significant insight into understanding how the brain responds to injury.Read moreRead less
Placental nutrient transport shows how complex traits evolve. This project aims to use amino acid transport in the vertebrate placenta as a model to demonstrate how genes are recruited and modified to produce a major organ. Using an innovative combination of a new technology, selected reaction monitoring, and transcriptomic and molecular approaches, plus carefully selected Australian species pairs, this project will study the evolution of a complex trait (placental amino acid transport). The pr ....Placental nutrient transport shows how complex traits evolve. This project aims to use amino acid transport in the vertebrate placenta as a model to demonstrate how genes are recruited and modified to produce a major organ. Using an innovative combination of a new technology, selected reaction monitoring, and transcriptomic and molecular approaches, plus carefully selected Australian species pairs, this project will study the evolution of a complex trait (placental amino acid transport). The project will provide fundamental advances in our knowledge of the nutrient transport during pregnancy that is required to produce a healthy baby.Read moreRead less
Remotely sensed forest water use in space and time. Remotely sensed forest water use in space and time. This project aims to develop and apply new methods to scale forest water use from plot to catchment-level, using relationships between plot-level annual evapotranspiration and biophysical and biochemical properties of stands detectable by unmanned aircraft systems and other remote sensing platforms. Australia's water security depends on understanding how changes in forests from disturbance and ....Remotely sensed forest water use in space and time. Remotely sensed forest water use in space and time. This project aims to develop and apply new methods to scale forest water use from plot to catchment-level, using relationships between plot-level annual evapotranspiration and biophysical and biochemical properties of stands detectable by unmanned aircraft systems and other remote sensing platforms. Australia's water security depends on understanding how changes in forests from disturbance and climate change influence catchment water yields. This project will estimate water yields over time and space in ungauged catchments with disturbed eucalypt forests. This research is expected to enable more effective risk mitigation and planning for augmentations; improved fire management strategies; and better water management of the Murray Darling Basin.Read moreRead less
Investigating the molecular function of alpha-Haemoglobin stabilising protein. The research described in this proposal will provide new insights into haemoglobin regulation and redox chemistry in erythrocytes. Deregulation of these processes gives rise to a number of debilitating diseases, including varieties of anaemia and thalassaemia-in Australia it is estimated that 3% of the population could be carriers of b-thalassaemia mutations. Given the contribution of free aHb to the pathology of b-th ....Investigating the molecular function of alpha-Haemoglobin stabilising protein. The research described in this proposal will provide new insights into haemoglobin regulation and redox chemistry in erythrocytes. Deregulation of these processes gives rise to a number of debilitating diseases, including varieties of anaemia and thalassaemia-in Australia it is estimated that 3% of the population could be carriers of b-thalassaemia mutations. Given the contribution of free aHb to the pathology of b-thalassaemia, understanding the specific aHb-binding factor, AHSP is a goal of national significance. In the long term, manipulation of AHSP function through gene therapy may have a direct role in the treatment of thalassaemia.Read moreRead less
Redefining the metallothionein's role in the injured brain: extracellular metallothioneins play an important role in astrocyte-neuron responses to injury. This project is being performed by an Australian team of researchers who are leaders in this field of research, and has significant national benefits in supporting this team reveal fundamental information on the cellular interactions that occur between astrocytes and neurons within the injured brain. In national terms, it will contribute to th ....Redefining the metallothionein's role in the injured brain: extracellular metallothioneins play an important role in astrocyte-neuron responses to injury. This project is being performed by an Australian team of researchers who are leaders in this field of research, and has significant national benefits in supporting this team reveal fundamental information on the cellular interactions that occur between astrocytes and neurons within the injured brain. In national terms, it will contribute to the concerted effort by Australian scientists to understand how and why neurons die following brain injury or neurodegenerative disease. Furthermore, this research contributes directly to the Designated National Research Priorities by identifying some of the earliest biochemical and cellular processes associated with aging or disease of the brain.Read moreRead less
Lags and legacies: antecedent effects on grassland biomass response to carbon dioxide. This project aims to assess how past conditions influence grassland responses to the rising atmospheric concentration of carbon dioxide. High CO2 concentrations should stimulate productivity but in grasslands this is rarely realised because other, mostly unknown, factors constrain the response. By synthesising data from past experiments, this project aims to determine exactly why grasslands fail to realise the ....Lags and legacies: antecedent effects on grassland biomass response to carbon dioxide. This project aims to assess how past conditions influence grassland responses to the rising atmospheric concentration of carbon dioxide. High CO2 concentrations should stimulate productivity but in grasslands this is rarely realised because other, mostly unknown, factors constrain the response. By synthesising data from past experiments, this project aims to determine exactly why grasslands fail to realise the full productivity benefits of increased CO2 and when this will happen. This should improve predictions of carbon exchange and indicating the best direction for climate change adaptation measures.Read moreRead less
Dynamic resilience and stability properties of marine systems: the importance of environment-engineer feedbacks in kelp forests. Kelp forests form complex habitats that support diverse, productive and economically important food-webs. This project will determine whether healthy kelp forests engineer their environment to make conditions more suitable for their continued recruitment and survivorship, thus increasing their stability and resilience in response to anthropogenic threats.
Utilising plant-sediment-feedbacks to enhance seagrass restoration. This project aims to investigate the role of sediment microbes in promoting the health of threatened seagrass species across Australia. This project expects to create new knowledge for enhancing restoration success for seagrasses by integrating macro and micro-ecology, environmental genomics, plant ecology and ecosystem function (e.g. nutrient and biogeochemistry cycling). Expected outcomes are new knowledge to enhance seagrass ....Utilising plant-sediment-feedbacks to enhance seagrass restoration. This project aims to investigate the role of sediment microbes in promoting the health of threatened seagrass species across Australia. This project expects to create new knowledge for enhancing restoration success for seagrasses by integrating macro and micro-ecology, environmental genomics, plant ecology and ecosystem function (e.g. nutrient and biogeochemistry cycling). Expected outcomes are new knowledge to enhance seagrass restoration utilising sediment microbes that can be integrated into management and policy. This project should provide significant benefits, such as the development of key strategic alliances to enhance management of seagrasses, and the ecosystem services, and economic and social benefits they provide.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE170100075
Funder
Australian Research Council
Funding Amount
$315,000.00
Summary
Acoustic liquid handling robotics for bioactive compound discovery. This project aims to use a Labcyte Echo 550 acoustic dispenser with Combination Software to deliver sophisticated assay-ready screening. The Echo is the only liquid handling dispenser for 1536-well microplates and will allow Australian researchers to develop assay miniaturisation. The robotics will provide our nation’s researchers with a distinct competitive edge by enhancing assay sophistication, accuracy and reproducibility wh ....Acoustic liquid handling robotics for bioactive compound discovery. This project aims to use a Labcyte Echo 550 acoustic dispenser with Combination Software to deliver sophisticated assay-ready screening. The Echo is the only liquid handling dispenser for 1536-well microplates and will allow Australian researchers to develop assay miniaturisation. The robotics will provide our nation’s researchers with a distinct competitive edge by enhancing assay sophistication, accuracy and reproducibility while reducing cost. The expected benefits will advance the elucidation of molecular mechanisms involved in complex biological phenomena. The benefits of this are substantial, including reduction in test compound and reagents, which in turn reduces laboratory costs, conserves cells and increases data quality.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE200100043
Funder
Australian Research Council
Funding Amount
$389,000.00
Summary
Rapid Molecular (Bio)material Imaging by Infrared and Raman Microscopies. This project aims to undertake fast probe-free biochemical/chemical imaging of heterogeneity within cells and materials surfaces with new infrared and Raman imaging. It will generate new fundamental knowledge on: cell heterogeneity and dynamic processes; technologies for optimising cell printing; understanding toxicity of microplastics; and protocols for measuring materials of technological relevance. Expected outcomes inc ....Rapid Molecular (Bio)material Imaging by Infrared and Raman Microscopies. This project aims to undertake fast probe-free biochemical/chemical imaging of heterogeneity within cells and materials surfaces with new infrared and Raman imaging. It will generate new fundamental knowledge on: cell heterogeneity and dynamic processes; technologies for optimising cell printing; understanding toxicity of microplastics; and protocols for measuring materials of technological relevance. Expected outcomes include: interdisciplinary collaborations in new protocols for in-vitro drug development; cell printing technologies; environmental impacts of microplastics; and materials design. Expected benefits include innovative approaches to early stage drug design; improved environmental controls and advances in innovative materials.Read moreRead less