Discovery Early Career Researcher Award - Grant ID: DE120102503
Funder
Australian Research Council
Funding Amount
$375,000.00
Summary
Circulating tumor cell isolation and detection: an integrated microfluidic capture device based on alternating current (AC) electrohydrodynamics. The detection of circulating tumor cells in clinical samples plays a critical role in cancer diagnosis and management. This project aims to develop microfluidic technology by incorporating AC electric field-induced vortices with three-dimensional microstructured electrodes that will advance our ability to analyse rare cells and proteins in clinical sam ....Circulating tumor cell isolation and detection: an integrated microfluidic capture device based on alternating current (AC) electrohydrodynamics. The detection of circulating tumor cells in clinical samples plays a critical role in cancer diagnosis and management. This project aims to develop microfluidic technology by incorporating AC electric field-induced vortices with three-dimensional microstructured electrodes that will advance our ability to analyse rare cells and proteins in clinical samples.Read moreRead less
Tuneable “Nano-Shearing”: An Innovative Mechanism for the Accurate and Specific Capture of Cells and Molecules. Recent investigations have discovered a tuneable electro-hydrodynamic force which drives lateral fluid motion within a few nanometers of an electrode surface. Because the magnitude of this fluid shear force can be tuned externally (for example, via the application of an AC electric field), it provides a new capability to physically displace weakly (non-specifically) bound cellular and ....Tuneable “Nano-Shearing”: An Innovative Mechanism for the Accurate and Specific Capture of Cells and Molecules. Recent investigations have discovered a tuneable electro-hydrodynamic force which drives lateral fluid motion within a few nanometers of an electrode surface. Because the magnitude of this fluid shear force can be tuned externally (for example, via the application of an AC electric field), it provides a new capability to physically displace weakly (non-specifically) bound cellular and molecular analytes. By performing research to further understand and develop this tuneable effect, this project aims to build and test a new platform technology to enable highly efficient capture and specific detection of low concentration pathogenic molecules and circulating tumour cells (CTCs).Read moreRead less
Milk protein profiling powered by multiplexed single molecule assay. This project aims to develop a novel device, comprising advanced single molecule imaging, microfluidics and immunoassay technologies, for quantification of milk protein variants. Milk quality is central to dairying and variants of proteins in milk affect its market value. No current milk protein detection technologies are readily applied in milk production quality control. This project aims to produce a device that can be used ....Milk protein profiling powered by multiplexed single molecule assay. This project aims to develop a novel device, comprising advanced single molecule imaging, microfluidics and immunoassay technologies, for quantification of milk protein variants. Milk quality is central to dairying and variants of proteins in milk affect its market value. No current milk protein detection technologies are readily applied in milk production quality control. This project aims to produce a device that can be used by milk producers and farms to profile protein variants with high sensitivity in a single test in an hour and screen unwanted protein contamination. The platform also has great potential for detecting other complex and low content analytes. It builds on innovations in nanoparticles and recent industry collaboration.Read moreRead less
Development of an ultrasensitive assay for human prion proteins. The aim of this work is to enable the detection of prion proteins in human blood and other tissues. The assay system to be developed will detect much lower levels of these disease-causing proteins than is possible at present; it will be more rapid and will measure prion protein levels more accurately than existing assays. The outcome of the work is expected to facilitate the production and certification of prion-free blood and bl ....Development of an ultrasensitive assay for human prion proteins. The aim of this work is to enable the detection of prion proteins in human blood and other tissues. The assay system to be developed will detect much lower levels of these disease-causing proteins than is possible at present; it will be more rapid and will measure prion protein levels more accurately than existing assays. The outcome of the work is expected to facilitate the production and certification of prion-free blood and blood products. Prions cause Bovine Spongiform Encaphalopathy and Creutzfeld Jacob Disease and attempts to control of these diseases would be greatly abetted by an optimal test for the disease-causing agent.Read moreRead less
Self-assembled phases as effective and selective materials for analysis. This project aims to develop a suite of self-assembled amphiphilic molecules to form distinctive materials for analytical chemistry. The cost-effective, easy to prepare, selective and environmentally benign materials will be for non-volatile bioactive and chiral molecular targets that are commonly encountered every day or are difficult to study. The project will open a new research area and will further uplift the internati ....Self-assembled phases as effective and selective materials for analysis. This project aims to develop a suite of self-assembled amphiphilic molecules to form distinctive materials for analytical chemistry. The cost-effective, easy to prepare, selective and environmentally benign materials will be for non-volatile bioactive and chiral molecular targets that are commonly encountered every day or are difficult to study. The project will open a new research area and will further uplift the international standing of Australia in the field. The project will provide a high level of training and expertise for Australia-based chemists.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE130100800
Funder
Australian Research Council
Funding Amount
$375,000.00
Summary
Polymer micropatches applied to the skin for integrated capture and detection of circulating biomarkers. The purpose of this project is to develop a rapid and integrated technology for user-friendly biomarker detection at the point-of-care. We expect the device to rapidly detect proteins and/or antibodies, without the need for highly trained health workers or access to scientific laboratories.
Discovery Early Career Researcher Award - Grant ID: DE140101056
Funder
Australian Research Council
Funding Amount
$380,156.00
Summary
Rational Design of Plasmonic Nanoassemblies for Rapid and Multiplexed Point-of-Care Diagnosis by Surface-enhanced Raman Spectroscopy (SERS). The central aim of this project is to develop a novel technology/sensor platform for rapid, quantitative, multiplexed and highly sensitive point-of-care diagnostics using surface-enhanced Raman spectroscopy (SERS) as the read-out approach. Three-dimensional plasmonic superstructures as novel SERS labels will be synthesised and characterised at single-partic ....Rational Design of Plasmonic Nanoassemblies for Rapid and Multiplexed Point-of-Care Diagnosis by Surface-enhanced Raman Spectroscopy (SERS). The central aim of this project is to develop a novel technology/sensor platform for rapid, quantitative, multiplexed and highly sensitive point-of-care diagnostics using surface-enhanced Raman spectroscopy (SERS) as the read-out approach. Three-dimensional plasmonic superstructures as novel SERS labels will be synthesised and characterised at single-particle level and the choice of optimal SERS-active three-dimensional superstructures for use will be guided by empirical structure-activity correlations in combination with computer simulations. Tumour biomarkers for breast cancer will be employed as the model target for establishing the detection platform in a portable configuration for point-of-care diagnostics.Read moreRead less
Integrated Nanoplatform for Multiomics Analysis of Cell-to-Cell Interaction. This project aims to develop an integrated nanoplatform for analysis of exosomes produced by host-pathogen interaction at the single cell level. This will be accomplished by engineering an innovative device involving plasmonic nanoparticles to probe exosomes molecular profiles over time. The intended outcome is a generic and robust platform for detailed molecular analysis of the consequences of cell-to-cell interactions ....Integrated Nanoplatform for Multiomics Analysis of Cell-to-Cell Interaction. This project aims to develop an integrated nanoplatform for analysis of exosomes produced by host-pathogen interaction at the single cell level. This will be accomplished by engineering an innovative device involving plasmonic nanoparticles to probe exosomes molecular profiles over time. The intended outcome is a generic and robust platform for detailed molecular analysis of the consequences of cell-to-cell interactions. Single cell scale will greatly improve detection accuracy for heterogeneous cell populations. Benefits will include new knowledge of cell-to-cell communication and intellectual property in manufacturing, which will foster collaborations across institutions and Australian industry by providing new technological solutions.Read moreRead less
Solving the problem of detecting small molecules in complex samples: A Label-Free Electrochemical Immuno-biosensor for drugs and pesticides. Biosensors are portable analytical devices which can be used by the general public without specialist training. The proposed research will develop a biosensor for the detection of small molecules such as pesticides, poisons and drugs; a class of analytes where there is currently no viable biosensor technology. The simple to use device will benefit the Aus ....Solving the problem of detecting small molecules in complex samples: A Label-Free Electrochemical Immuno-biosensor for drugs and pesticides. Biosensors are portable analytical devices which can be used by the general public without specialist training. The proposed research will develop a biosensor for the detection of small molecules such as pesticides, poisons and drugs; a class of analytes where there is currently no viable biosensor technology. The simple to use device will benefit the Australian community by providing technology which will allow rapid and inexpensive monitoring of water as well as biomedical diagnosis. The research will also benefit Australia via providing the training of scientists to establish the new generation of Australia's bionanotechnology industry. Read moreRead less
Industrial Transformation Training Centres - Grant ID: IC220100035
Funder
Australian Research Council
Funding Amount
$4,958,927.00
Summary
ARC Training Centre for Hyphenated Analytical Separation Technologies . The toughest analytical science challenges typically require advanced analytical technologies to acquire the desired solutions. In the field of separation science this inevitably involves hyphenated separation technologies, specifically the combination of chromatography and mass spectrometry. Advancing this technology to its full capability requires the collaborative strength of academic, industry and end-user partnerships, ....ARC Training Centre for Hyphenated Analytical Separation Technologies . The toughest analytical science challenges typically require advanced analytical technologies to acquire the desired solutions. In the field of separation science this inevitably involves hyphenated separation technologies, specifically the combination of chromatography and mass spectrometry. Advancing this technology to its full capability requires the collaborative strength of academic, industry and end-user partnerships, providing the materials and inspiration for young researchers to apply novel hyphenated methods to complex environmental and industrial systems. This Centre will deliver fundamental developments in hyphenated technologies, new analytical capability, and applied outcomes across multiple end-user groups and interests. Read moreRead less