Determinants Of Occurrence And Progression Of Aortic Stenosis In Bicuspid And Tricuspid Valves.
Funder
National Health and Medical Research Council
Funding Amount
$546,394.00
Summary
Aortic stenosis (AS) is a major cause of cardiac failure and mortality in ageing Australians. AS occurs both in bicuspid aortic valves (BAV;0.5-1% of the population) and in anatomically tricuspid valves. There is an associated incremental risk of myocardial infarction. The research will investigate patients with BAV and with early AS in tricuspid valves, in order to examine the potential roles of loss of nitric oxide effect and of oxidant stress as predictors of AS development.
Characterisation Of A Novel Direct Electrochemical Chip As A Biosensor And Tool For Studying Redox-sensitive Proteins
Funder
National Health and Medical Research Council
Funding Amount
$144,500.00
Summary
Biosensors use biomolecules to detect a chemical event. They are becoming important for the rapid and reliable measurement of the concentrations of molecules in fluids. In human medicine they will be of great use to general practitioners and patients for instantaneous read outs of concentrations of many different biological molecules. How well a biosensor responds depends on the method in which the biomolecule is immobilised to a surface and the signal detected. We have made a significant advanc ....Biosensors use biomolecules to detect a chemical event. They are becoming important for the rapid and reliable measurement of the concentrations of molecules in fluids. In human medicine they will be of great use to general practitioners and patients for instantaneous read outs of concentrations of many different biological molecules. How well a biosensor responds depends on the method in which the biomolecule is immobilised to a surface and the signal detected. We have made a significant advance in biosensing capabilities using a recombinant protein (thioredoxin) and demonstrated the improvement that is possible by (i) immobilising the protein in a highly oriented way and (ii) using a sensitive electrical signal to monitor the response. Here we will undertake more comprehensive testing by extending the number of proteins to include the 4 major classes of redox-sensitive biomolecules (proteins) in the body. This will enable us to establish the broad application of our methods and substantially improve our ability to commercialize our discoveries.Read moreRead less