New Technologies For Hierarchial Shotgun Sequencing Of Recalcitrant And Repetitive DNA
Funder
National Health and Medical Research Council
Funding Amount
$79,750.00
Summary
Many repetitive regions of genomes are difficult to sequence and to assemble. �Sequencing Aided by Mutation� (SAM) is a new sequencing technology, which overcomes many of the difficulties that hinder current sequencing methods. SAM involves forming randomly mutated copies of the target DNA. These copies can then be sequenced and new mathematical tools permit the original target sequence to be revealed from the mutant copies. Here the technology will be developed to aid sequencing of long repetit ....Many repetitive regions of genomes are difficult to sequence and to assemble. �Sequencing Aided by Mutation� (SAM) is a new sequencing technology, which overcomes many of the difficulties that hinder current sequencing methods. SAM involves forming randomly mutated copies of the target DNA. These copies can then be sequenced and new mathematical tools permit the original target sequence to be revealed from the mutant copies. Here the technology will be developed to aid sequencing of long repetitive DNA fragments in genomes.Read moreRead less
Retroviral Expression Cloning Using An Arrayed Full Length CDNA Gene Set
Funder
National Health and Medical Research Council
Funding Amount
$1,841,500.00
Summary
The sequencing of the human genome has revealed the blueprint for life, but the identities and-or functions of the majority of genes remain unknown. Here we propose to establish a radically modified retroviral expression cloning system that will, in principle, allow identification of all genes that confer a particular dominant phenotype. To do this we will establish an arrayed retroviral library of sequence-verified genes covering the entire human transcriptome. This technology will be used to i ....The sequencing of the human genome has revealed the blueprint for life, but the identities and-or functions of the majority of genes remain unknown. Here we propose to establish a radically modified retroviral expression cloning system that will, in principle, allow identification of all genes that confer a particular dominant phenotype. To do this we will establish an arrayed retroviral library of sequence-verified genes covering the entire human transcriptome. This technology will be used to identify genes involved in a wide range of medically-important biological processes.Read moreRead less
High blood pressure affects 1 in 5 Australian adults and is a leading cause of mortality and morbidity from heart attack and stroke. The condition tends to run in families and genetic predisposition, in the face of environmental factors, leads to the elevation in blood pressure. My Lab has demonstrated the capacity of a cohort of affected hypertensive sibships we have collected to find loci for essential hypertension at a level that has achieved genome-wide statistical significance and has been ....High blood pressure affects 1 in 5 Australian adults and is a leading cause of mortality and morbidity from heart attack and stroke. The condition tends to run in families and genetic predisposition, in the face of environmental factors, leads to the elevation in blood pressure. My Lab has demonstrated the capacity of a cohort of affected hypertensive sibships we have collected to find loci for essential hypertension at a level that has achieved genome-wide statistical significance and has been published in a leading molecular genetics journal. Moreover, this previous work, which included fine-mapping after finding a suggestive locus following a scan of chromosome 1, not only demonstrated significant linkage, but also went on to compare gene markers between a different cohort of (unrelated) hypertensive subjects with 2 affected parents (and early-onset, moderate to severe hypertension) and control normotensive matched subjects with unaffected parents, to identify a likely candidate gene. This same approach will be used to complete the rest of the genome. The discovery of all of the genes for essential hypertension will be an important prelude to: (1) developing new, more effective treatments, since the gene products responsible will be able to be targetted by novel therapeutics, (2) genotyping individuals early in life in order to advise them what their risk is, and thus allow couselling about lifestyle modification, (3) more logically apply existing treatment strategies according to the volume-neural-vasoconstrctor component of the contribution to high blood pressure.Read moreRead less
Improving Health Outcomes With Population-based Biological, Social And Environmental Information And Research Methods.
Funder
National Health and Medical Research Council
Funding Amount
$3,050,000.00
Summary
This proposal brings together two partner institutions and research teams in an integrated program through pooling of experience, sharing of databases and other resources and joint mentoring of new researchers to achieve improved health status through a whole of life and intergenerational view of health and health determinants. The proposal incorporates real consumer participation throughout the grant. The grant will help position Australia in the newly emerging research skills and methodology i ....This proposal brings together two partner institutions and research teams in an integrated program through pooling of experience, sharing of databases and other resources and joint mentoring of new researchers to achieve improved health status through a whole of life and intergenerational view of health and health determinants. The proposal incorporates real consumer participation throughout the grant. The grant will help position Australia in the newly emerging research skills and methodology involved with data linkages. Building on the successful multi-disciplinary research environments at both institutions, the grant will provide additional funds for these two groups to work cooperatively together. The groups currently operate with established links to researchers on a national basis and will be exploring aspects from the biomedical, clinical and health service delivery perspective to link environmental and social determinants of health from early childhood to adulthood. The grant will provide research training in a whole of life approach. In particular, researchers will gain experience in linking research findings into health policy with active consumer involvement to improve health outcomes.Read moreRead less