Identification of nuclear reprogramming factors in oocyte cytoplasm. The mature oocyte contains dominant factors that are capable of erasing tissue specific gene expression profiles of somatic cells. These reprogramming factors would be valuable for dedifferentiation of cells and for nuclear transfer in animal cloning. The research involves determination of reprogramming factors present in active cytoplasm following enucleation of the germinal vesicle, blockage of transcription and translation, ....Identification of nuclear reprogramming factors in oocyte cytoplasm. The mature oocyte contains dominant factors that are capable of erasing tissue specific gene expression profiles of somatic cells. These reprogramming factors would be valuable for dedifferentiation of cells and for nuclear transfer in animal cloning. The research involves determination of reprogramming factors present in active cytoplasm following enucleation of the germinal vesicle, blockage of transcription and translation, and timed cultures. The assays will involve maintenance of reprogramming ability and erasure of somatic gene transcription. By subtractive elimination the function of isolated proteins which are involved in reprogramming will be identified for potential recombinant production.Read moreRead less
Use of Gradipore technology to develop novel methods for the preparation and segregation of mammalian spermatozoa. The purpose of this project is to harness the expertise available within an Australian biotechnology company, Gradipore, to develop novel methods for the preparation of mammalian spermatozoa and the segregation of these cells into X-and Y- bearing populations. This technology will find application in: (1)clinical andrology, where rapid, safe protocols for the preparation and segrega ....Use of Gradipore technology to develop novel methods for the preparation and segregation of mammalian spermatozoa. The purpose of this project is to harness the expertise available within an Australian biotechnology company, Gradipore, to develop novel methods for the preparation of mammalian spermatozoa and the segregation of these cells into X-and Y- bearing populations. This technology will find application in: (1)clinical andrology, where rapid, safe protocols for the preparation and segregation of human spermatozoa are being actively sought in the context of assisted conception and the management of sex-linked genetic diseases and (2) agriculture, particularly the cattle industry, where a capacity to predetermine the sex of the offspring would be extremely valuable.Read moreRead less
The Relevance of the Bovine Major Histocompatibility Complex (MHC) to Milk Production. The objective of this project is to provide the dairy industry with an integrated package that includes a ?dairy performance database? linked to DNA-based information to complement existing breeding practices. Our intention is increase the profitability of the dairy industry, hence making a dairy farm a sustainable venture in a deregulated environment. Profitability results from a combination of several fact ....The Relevance of the Bovine Major Histocompatibility Complex (MHC) to Milk Production. The objective of this project is to provide the dairy industry with an integrated package that includes a ?dairy performance database? linked to DNA-based information to complement existing breeding practices. Our intention is increase the profitability of the dairy industry, hence making a dairy farm a sustainable venture in a deregulated environment. Profitability results from a combination of several factors. The drivers of the industry include political, market, environmental, husbandry, nutrition, and genetic factors. These factors are interrelated. In this proposal, we are specifically interested in the relationship between milk yields and the DNA profile of dairy cattle.Read moreRead less