Targeting and stabilizing proteins in sugar storage vacuoles for metabolic engineering in sugarcane. We have isolated a novel gene for an enzyme that efficiently converts sucrose into a product of much higher value. We have shown that the enzyme functions in sugarcane, a first example of the potential for new biosynthetic capacities in this highly productive crop. Because 90% of stored sucrose is in specialized vacuoles, the enzyme needs to be directed into these vacuoles, and made stable and ac ....Targeting and stabilizing proteins in sugar storage vacuoles for metabolic engineering in sugarcane. We have isolated a novel gene for an enzyme that efficiently converts sucrose into a product of much higher value. We have shown that the enzyme functions in sugarcane, a first example of the potential for new biosynthetic capacities in this highly productive crop. Because 90% of stored sucrose is in specialized vacuoles, the enzyme needs to be directed into these vacuoles, and made stable and active there. This is feasible by building on recent discoveries about vacuolar targeting in plants. The outputs include scientific understanding to underpin metabolic engineering in plants, and a profitable high-technology export industry for Australia.Read moreRead less
Optimising transgene expression and stability for enhanced sugar yield and high-value sugar production in sugarcane. 'SugarBooster' technology has the potential to underpin a value-added sugarcane industry. Higher sucrose yield is a key to sustainable export profitability, and it makes the development of renewable biofuels from sugarcane more feasible. Isomaltulose has established health benefits for consumers and it is also attractive as a renewable starting material for industry. But it must c ....Optimising transgene expression and stability for enhanced sugar yield and high-value sugar production in sugarcane. 'SugarBooster' technology has the potential to underpin a value-added sugarcane industry. Higher sucrose yield is a key to sustainable export profitability, and it makes the development of renewable biofuels from sugarcane more feasible. Isomaltulose has established health benefits for consumers and it is also attractive as a renewable starting material for industry. But it must currently be produced by expensive fermentation. Efficient production in plants will open an increasing world market. This collaborative project is vital to bring these breakthrough technologies to reliable commercial implementation, in time to capture the economic benefits of the protected IP for Australia.Read moreRead less
Combining molecular plant physiology and breeding to improve canola (Brassica napus) performance in dry environments. Canola is Australia's most important oilseed crop with 1,400,000 hectares sown annually worth $560m. The major abiotic factor limiting canola production in Australia is water availability. Transpiration efficiency (TE) is a trait with potential to contribute to improved drought tolerance of grain crops. This project seeks to select canola germplasm with improved TE by indirect s ....Combining molecular plant physiology and breeding to improve canola (Brassica napus) performance in dry environments. Canola is Australia's most important oilseed crop with 1,400,000 hectares sown annually worth $560m. The major abiotic factor limiting canola production in Australia is water availability. Transpiration efficiency (TE) is a trait with potential to contribute to improved drought tolerance of grain crops. This project seeks to select canola germplasm with improved TE by indirect selection for carbon-isotope-discrimination. In addition we will use the extensive Brassica-Arabidopsis genome synteny to locate and alter the expression of genes involved in TE using Arabidopsis as a model. The longterm aim is to improve the reliability and overall grain production of canola in Australia. Read moreRead less
Plant heterotrimeric G proteins: new roles in defence, stomatal control and ABA perception. Agriculture is an important economic activity in Australia that results in considerable export revenues. Two of the major problems facing agriculture around the globe are the incidence of diseases and the scarcity of water. Agricultural losses caused by plant pathogens and low water availability account for billions of dollars every year and have profound economic and social implications. Water is an extr ....Plant heterotrimeric G proteins: new roles in defence, stomatal control and ABA perception. Agriculture is an important economic activity in Australia that results in considerable export revenues. Two of the major problems facing agriculture around the globe are the incidence of diseases and the scarcity of water. Agricultural losses caused by plant pathogens and low water availability account for billions of dollars every year and have profound economic and social implications. Water is an extremely scarce resource in Australia and periodic droughts inflict immense losses to the Australian agricultural sector. Our research will explore new and cleaner strategies to provide crop protection as well as to increase water use efficiency.Read moreRead less
NextGen Sorghum: Genomic approaches to novel renewable bioproducts. Next Gen Sorghums will have enhanced nutritional and processing qualities for humans and animals, and be ideal feedstocks for the bio-economy for the delivery of novel products. Our approaches in reverse genetics to identify gene networks which control sorghum seed development, cell size, cell wall thickness and the way in which starch and protein are packaged within the grain will generate knowledge to underpin the future utili ....NextGen Sorghum: Genomic approaches to novel renewable bioproducts. Next Gen Sorghums will have enhanced nutritional and processing qualities for humans and animals, and be ideal feedstocks for the bio-economy for the delivery of novel products. Our approaches in reverse genetics to identify gene networks which control sorghum seed development, cell size, cell wall thickness and the way in which starch and protein are packaged within the grain will generate knowledge to underpin the future utilisation of this important grain. This will help to drive the future of Australian cereals industries, with health benefits to consumers, the enhanced delivery of specialised feedstocks for novel and renewable bio-products, and financial benefits to farmers.Read moreRead less
Systems biology to integrate genomics into crop improvement programs. Plant breeding programs have not reaped the benefits of the revolution in molecular genetic technologies. This systems biology project will develop mathematical models of plants to span levels of biological organisation from gene to whole organism. It will focus on understanding and modelling the genetics and physiology of key adaptive traits in sorghum and maize. It will use computer simulation to employ resultant gene-to- ....Systems biology to integrate genomics into crop improvement programs. Plant breeding programs have not reaped the benefits of the revolution in molecular genetic technologies. This systems biology project will develop mathematical models of plants to span levels of biological organisation from gene to whole organism. It will focus on understanding and modelling the genetics and physiology of key adaptive traits in sorghum and maize. It will use computer simulation to employ resultant gene-to-phenotype models in ways that will underpin a major shift in how plant breeding programs operate. This new integrating technology will lead to more rapid advance in breeding better adapted and higher yielding crops.Read moreRead less
eSorghum as a bio-fuel feedstock for arid environments. Increasing fuel costs, finite resources and the need to develop more carbon neutral and cleaner fuels have created a need for renewable sources. Ethanol and future generation biofuels (butanol and more energy-rich alcohols) can be extracted from biomass sources. Sorghum is an ideal bioenergy feedstock in the hotter, drier areas of Northern Australia, where starch (grain), sugar and lignocellulose (stover) can be amassed in this water effi ....eSorghum as a bio-fuel feedstock for arid environments. Increasing fuel costs, finite resources and the need to develop more carbon neutral and cleaner fuels have created a need for renewable sources. Ethanol and future generation biofuels (butanol and more energy-rich alcohols) can be extracted from biomass sources. Sorghum is an ideal bioenergy feedstock in the hotter, drier areas of Northern Australia, where starch (grain), sugar and lignocellulose (stover) can be amassed in this water efficient plant. Identifying and manipulating the genes to enable the improvement of sorghum as a dedicated bioenergy crop, will enable the increased efficiency of the Australian biofuels industry and create sustainable rural industries.Read moreRead less
Special Research Initiatives - Grant ID: SR0354766
Funder
Australian Research Council
Funding Amount
$10,000.00
Summary
Molecular farming: seizing the opportunity to establish a new technology based, value adding industry in and for Australia. Molecular farming involves the production of novel products in plants and brings together the technology to genetically modify plants to produce these products with efficient production of plant biomass. The range of potential products is very broad and includes therapeutic and industrial proteins, bioplastics, fibres, food additives, glues and dyes. The molecular farming i ....Molecular farming: seizing the opportunity to establish a new technology based, value adding industry in and for Australia. Molecular farming involves the production of novel products in plants and brings together the technology to genetically modify plants to produce these products with efficient production of plant biomass. The range of potential products is very broad and includes therapeutic and industrial proteins, bioplastics, fibres, food additives, glues and dyes. The molecular farming industry is already established in North America with products on the market. Australia has an outstanding opportunity to participate in this new industry as a producer if we can aggregate the critical scientific mass and work together with the current fledgling industry and State and Federal regulators.Read moreRead less