ARC Centre of Excellence in Plants for Space. ARC Centre of Excellence in Plants for Space. This Centre aims to create on-demand, zero-waste, high-efficiency plants and plant products to address grand challenges in sustainability for Space and on Earth. Significant advances in plant, food, and sensory science; process and systems engineering; law and policy; and psychology are expected to deliver transformative solutions for Space habitation – and create enhanced plant-derived food and bioresour ....ARC Centre of Excellence in Plants for Space. ARC Centre of Excellence in Plants for Space. This Centre aims to create on-demand, zero-waste, high-efficiency plants and plant products to address grand challenges in sustainability for Space and on Earth. Significant advances in plant, food, and sensory science; process and systems engineering; law and policy; and psychology are expected to deliver transformative solutions for Space habitation – and create enhanced plant-derived food and bioresources to capitalise upon emergent and rapidly expanding domestic and global markets. Anticipated outcomes include industry uptake of innovative plant forms, foods, technologies, and commodities; and an ambitious education and international co-ordination agenda to position Australia as a global leader in research supporting Space habitation.Read moreRead less
Risk Profile For Paralytic Shellfish Toxins In Tasmanian Periwinkles
Funder
Fisheries Research and Development Corporation
Funding Amount
$180,504.00
Summary
Recurrent harmful algal blooms in Tasmanian waters (Gymnodinium in the South and Alexandrium on the East Coast) have impacted Oyster, Mussel, Abalone and Rock Lobster industries, necessitating the implementation of biotoxin monitoring programs and harvest closures during high risk periods. It is currently unknown whether Periwinkles can accumulate paralytic shellfish toxins (PST) from Tasmanian microalgal blooms and a conservative risk management approach has been taken thus far to protect both ....Recurrent harmful algal blooms in Tasmanian waters (Gymnodinium in the South and Alexandrium on the East Coast) have impacted Oyster, Mussel, Abalone and Rock Lobster industries, necessitating the implementation of biotoxin monitoring programs and harvest closures during high risk periods. It is currently unknown whether Periwinkles can accumulate paralytic shellfish toxins (PST) from Tasmanian microalgal blooms and a conservative risk management approach has been taken thus far to protect both public health and market access.
Unlike bivalve shellfish, Abalone and Rock Lobster, there is currently no clearly defined biotoxin management plan for Periwinkles in Tasmania. As grazers, Periwinkles are loosely grouped with Urchins and Abalone, the latter of which are a proven PST risk. This Abalone biotoxin sampling plan is triggered when Tasmanian Shellfish Market Access Program (ShellMAP) closes bivalve harvest areas due to PST risk. In a conservative approach, the current recommendation is PST analysis of 20 pooled Periwinkles per Abalone block on at least a monthly basis to maintain market access. This presents a considerable cost burden to the small dive fishery and interrupts harvest operations, as PST sampling of Periwinkles is required if elevated PST are detected in Abalone, which are known to hold onto PST for longer time periods in between blooms (years).
To date, the vast majority of Periwinkle samples that have been analysed for PST were collected from weak or no bloom years or regions. Evidence from French tank trials exposing the common European Periwinkle (Littorina littorea) to Alexandrium minutum cysts containing PST showed significant toxin uptake (0.2 mg PST/kg) within six days of exposure (Neves et al, 2015). It remains unknown whether commercially harvested Tasmanian Periwinkles (Lunella undulata) are at risk of PST accumulation and if the current conservative monitoring approach is commensurate to risk. In such scenarios, risk managers will commonly outsource a preliminary risk assessment (known as a risk profile). Risk profiles are an important tool for risk managers and industry. They provide a summary of all information pertinent to food safety associated with the specific hazard/food combination. The purpose of a risk profile is to assist initial risk management activities, such as identifying future actions required (if any), and the options for food safety management programs. They also inform the level of resourcing required to control the hazard/food pairing.
This project will deliver an urgently required risk profile for PST in Periwinkles to inform future actions (if any) and provide options for cost-effective food safety management programs. This profile will: 1. Determine the risk of PST accumulation in Periwinkles in Tasmania as a result of harmful microalgal blooms during high risk exposures at peak algal bloom densities in the field and in tank trials. 2. Outline potential management options and knowledge gaps, should risk management be necessary. This includes an assessment of whether the bivalve PST regulatory limit is appropriate to use in Periwinkle risk management (currently based on shellfish serving size of 100-400g) and the spatial variability associated with sampling.
References Neves, Raquel AF, et al. "Responses of the common periwinkle Littorina littorea to exposure to the toxic dinoflagellate Alexandrium minutum." Journal of Molluscan Studies 81.2 (2015): 308-311.
Objectives: 1. Determine whether Tasmanian Periwinkles can bioaccumulate PST from Alexandrium and/or Gymnodinium microalgal blooms during both field and laboratory exposures. 2. Identify the most suitable size of biotoxin management zones for Periwinkles. 3. Assess whether the use of the bivalve PST regulatory limit is appropriate for Periwinkles by conducting a literature search and consumption survey. 4. Generate a risk profile for PST in Tasmanian wild caught Periwinkles and provide guidance for risk management. Read moreRead less
Protein biosensors for detecting smoke exposure of grapes. Bush fires and controlled burns that take place in the vicinity of vineyards can lead to grape contamination with tasteless phenolic glucosides. Their hydrolysis during wine making leads to “smoke taint” – an unpleasant medicinal taste that can render wine undrinkable. We will apply a combination of organic synthesis, protein engineering and directed evolution to develop protein-based biosensors of phenolic glucosides. These biosensors w ....Protein biosensors for detecting smoke exposure of grapes. Bush fires and controlled burns that take place in the vicinity of vineyards can lead to grape contamination with tasteless phenolic glucosides. Their hydrolysis during wine making leads to “smoke taint” – an unpleasant medicinal taste that can render wine undrinkable. We will apply a combination of organic synthesis, protein engineering and directed evolution to develop protein-based biosensors of phenolic glucosides. These biosensors will be used to devise a simple portable colorimetric test that can be performed in the vineyard or the winery. The ability to rapidly determine the level of grape contamination with phenolic glucosides would give Australian wine growers and wine makers a powerful tool to mitigate the effects of bushfires.Read moreRead less
Preparing For Threats And Opportunities Of Alternative Proteins
Funder
Fisheries Research and Development Corporation
Funding Amount
$209,922.00
Summary
The demand for alternative proteins is increasing globally, and although the impact on the fishing and aquaculture sectors is lagging, it is an opportune time to review the potential risks and opportunities. This will help us reposition the sector to make use of the opportunities and mitigate the risks.
Our multidisciplinary team will deliver a detailed assessment of risks and opportunities, and engage stakeholders to understand their needs, concerns and impact pathways. We will use our ....The demand for alternative proteins is increasing globally, and although the impact on the fishing and aquaculture sectors is lagging, it is an opportune time to review the potential risks and opportunities. This will help us reposition the sector to make use of the opportunities and mitigate the risks.
Our multidisciplinary team will deliver a detailed assessment of risks and opportunities, and engage stakeholders to understand their needs, concerns and impact pathways. We will use our analysis to demonstrate the potential pathways they can use to make use of the opportunities and mitigate the risks.
Our project will: - Understand the opportunities and risks for the growing trend of alternative proteins on the fishing and aquaculture sectors and supply chain - Assess the potential impacts of alternative proteins on the fishing and aquaculture sectors and supply chain through scenario modelling - Engage deeply with fishing, aquaculture and related stakeholders to co-design interpretation of insights into risks and opportunities as well as formulating options and responses - Make recommendations on how fishing and aquaculture sectors and enterprises might re-position to embrace benefits associated with alternative proteins, and respond to associated risks.
Relevant outcomes: Outcome 1: Growth for enduring prosperity Outcome 3: A culture that is inclusive and forward thinking Outcome 5: Community trust, respect and value Enabling strategy III: Promote innovation and entrepreneurship Enabling Strategy IV: Build capability and capacity
Objectives: 1. Understand the opportunities and risks for the growing trend of alternative proteins on the fishing and aquaculture sectors and supply chain 2. Assess the potential impacts of alternative proteins on the fishing and aquaculture sectors and supply chain 3. Make recommendations on how fishing and aquaculture sectors and enterprises might re-position to embrace benefits associated with alternative proteins, and respond to associated risks. Read moreRead less
Clarence River Green Prawn Market Diversification Assistance Measures
Funder
Fisheries Research and Development Corporation
Funding Amount
$165,000.00
Summary
The Clarence River Region is known for its high-quality prawn markets. The region was kept profitable during COVID restrictions due to a significant portion of the fisher directing its product to the highly profitable bait market. This diversification away from the consumer market has ensured stability for the region. Many fishing businesses pivoted their strategies to meet this new buyer to the region. The loss of the uncooked prawn market in the Clarence region will therefore destabilize t ....The Clarence River Region is known for its high-quality prawn markets. The region was kept profitable during COVID restrictions due to a significant portion of the fisher directing its product to the highly profitable bait market. This diversification away from the consumer market has ensured stability for the region. Many fishing businesses pivoted their strategies to meet this new buyer to the region. The loss of the uncooked prawn market in the Clarence region will therefore destabilize the industry. The restriction of uncooked prawn trade therefore must be addressed through market research, diversification and activation. However, there is immediate need for alternative markets so an intense focused market activation and access is the key. PFA has identified key market persons that can create links between industry and high-end chefs to: 1. Identify alternative market uses that fall within the quarantine requirements 2. Start immediate market trials and activation 3. Review online presence and build industry skills to improve online presence for sales • It is intended that this will lead into immediate supply agreements to these alternative markets that will in turn remove burden on the existing cooked prawn market
Objectives: 1. To identify and trial supplies of Clarence River prawns to alternative market 2. To activate alternative markets to reduce negative impact of trade restriction to the Clarence River region 3. To build skills within local fishers to build online presence Read moreRead less
Detecting Paralytic Shellfish Toxins In Oysters - Initial Assessment Of AquaBC Rapid Test Kit
Funder
Fisheries Research and Development Corporation
Funding Amount
$29,845.00
Summary
Reliable detection of marine biotoxins is a critical requirement for any effective biotoxin monitoring program, requiring any analytical technique to be properly validated. The Neogen rapid test kit for the detection of paralytic shellfish toxin (PSTs) was successfully validated in both single lab and inter-lab validations for use in oysters, offering rapid (within 20 min) on farm results. The test was used in Tasmania to reduce business risk, (i.e. frequent testing of shellfish that can rapidly ....Reliable detection of marine biotoxins is a critical requirement for any effective biotoxin monitoring program, requiring any analytical technique to be properly validated. The Neogen rapid test kit for the detection of paralytic shellfish toxin (PSTs) was successfully validated in both single lab and inter-lab validations for use in oysters, offering rapid (within 20 min) on farm results. The test was used in Tasmania to reduce business risk, (i.e. frequent testing of shellfish that can rapidly accumulate PST within a week) and employed in South Australia for regulatory purposes (low frequency of PST detection in this region).
A key factor influencing the suitability of antibody based rapid test kits is the PST profile present in the sample to be analysed. The term PST profile describes the relative concentrations of different PST analogues that might be present in each seafood sample. These profiles differ between toxic algal species, different seafood species and their tissues. Not all PST analogues are equally detected by the antibodies of different test kits (quantified as the % cross-reactivity). These cross-reactivities are critical for ensuring reliable detection across different combinations of PST analogues that might be present in shellfish.
To determine if the AquaBC rapid test kit is suitable replacement for routine monitoring, a full validation for each seafood tissue matrix would normally be conducted to determine the probability of detection curve (probability of detection across a range of PST concentrations) for multiple PST profiles, ideally followed by an inter-laboratory validation (as was conducted for the Neogen test). However, a full validation study requires repeat testing of hundreds of samples and is therefore expensive. From our previous work with the Neogen test kit, we have identified some key performance indicators that would allow for a quick initial assessment of the new AquaBC test kit, using much reduced sample numbers. These small pilot trials would include testing the most commonly encountered PST profiles (i.e. which PST toxin analogues are present) and their relative concentrations (i.e. can we reliably detect the presence of PST at the regulatory level without obtaining too many positive results at PST levels below concern?). Rather than conducting a full validation, this project will provide an initial assessment of these parameters in regard to the PST profiles commonly encountered in TAS, SA and NSW. Should this assessment be positive, a full follow up validation may be recommended. Objectives: 1. Review & identify different PST profiles that may be encountered in TAS, SA and NSW oysters. 2. Challenge the AquaBC test kit against a range of different PST concentrations and profiles to provide an initial assessment of its suitability for detecting PST in Australian oyster tissues. Read moreRead less
Toxigenic Vibrio Baselines And Optimum Storage, Transport And Shelf-life Conditions To Inform Cold Supply Chains In The North Australian Tropical Rock Oyster Industry
Funder
Fisheries Research and Development Corporation
Funding Amount
$199,300.00
Summary
Internationally, Tropical Rock Oysters have a poor safety reputation with Vibrio at the top of the list. While a pro-active not reactive approach to vibrio food safety is essential for product assurance and branding, effort needs to be proportional to risk. And risk assessment also needs to be informed by real data. There are certainly knowledge gaps for north Australia, but we know seawater contains up to 42 Vibrio spp. including several known toxigenic species in addition to the human pathogen ....Internationally, Tropical Rock Oysters have a poor safety reputation with Vibrio at the top of the list. While a pro-active not reactive approach to vibrio food safety is essential for product assurance and branding, effort needs to be proportional to risk. And risk assessment also needs to be informed by real data. There are certainly knowledge gaps for north Australia, but we know seawater contains up to 42 Vibrio spp. including several known toxigenic species in addition to the human pathogens Vibrio parahaemolyticus (Vp) and V. vulnificus (Vv). We know Vp responds to temperature but Vv does not. And we know Vv concentrations in seawater are higher in the wet season compared to the dry, and more shellfish are Vp and Vv positive in the wet season. So if vibrio diversity and abundance in TRO is seasonal (as shown elsewhere), it is likely that Vibrio spp. infections in humans will also follow a seasonal trend which has implications for risk management. A major bottleneck is that we don’t know how vibrios respond to storage and transport temperatures in TRO. We know that the Pacific and Sydney Rocks respond differently so it is not ‘one size fits all’ and it is certain TROs will be different again. In addition to identifying vibrio baselines in TRO and developing tests for toxigenic species, we will identify the best post-harvest storage and transport temperatures and assess TRO shelf life at realistic storage temperatures. This will provide fundamental information to inform cold supply chains that will support farmers, wholesalers and retailers of TROs from north Australia. We can also use this information to prepare an appropriate and regionally relevant vibrio risk profile for TRO in northern Australia to assist initial risk management activities. This information will provide the developing TRO industry with the knowledge needed to ensure an exemplary reputation, thus giving access to premium markets.
Objectives: 1. Measure vibrio baseline in Tropical Rock Oysters and develop tests to vibrio species that are toxigenic to oysters and humans 2. Identify optimum storage and transport temperatures to inform post-harvest cold supply chains 3. Assess TRO shelf life at realistic storage temperatures to maximise product quality and inform cold supply chains 4. Use objective 1-3 outcomes to produce a risk profile for vibrio in north Australian TRO that will support the industry as it seeks to deliver a safe, premium product Read moreRead less
Where Should I Farm My Oysters? Does Natural Cadmium Distribution Restrict Oyster Farm Site Selection In The Northern Territory?
Funder
Fisheries Research and Development Corporation
Funding Amount
$123,272.00
Summary
RD&E that addresses critical hurdles to Aboriginal capacity and enterprise development (e.g. quality assurance strategies) have been identified as priority areas of the NT RAC and the Indigenous Reference Group (IRG). NT Fisheries has been conducting research to support Aboriginal aspirations to establish tropical oyster farms in the Northern Territory (NT).
Heavy metals have been a longstanding concern as an impediment to the development of a tropical oyster industry. Cadmium (Cd) bioa ....RD&E that addresses critical hurdles to Aboriginal capacity and enterprise development (e.g. quality assurance strategies) have been identified as priority areas of the NT RAC and the Indigenous Reference Group (IRG). NT Fisheries has been conducting research to support Aboriginal aspirations to establish tropical oyster farms in the Northern Territory (NT).
Heavy metals have been a longstanding concern as an impediment to the development of a tropical oyster industry. Cadmium (Cd) bioaccumulates in the tissue of oysters, and unlike E. coli or toxic algae, has a long depuration period. As a result Cd levels are a major determining factor on the saleability of farmed tropical oysters. McConchie, D.M & Lawrance, L.M (1991) and FRDC Project 2012-223 identified high Cd concentrations, which varied considerably across locations and water depth, in blacklip oysters (Saccostrea echinata) at location in Shark Bay, WA and South Goulburn Island, NT respectively. Following these projects naturally occurring heavy metals have been a presumed barrier to the establishment of an oyster industry in the NT, due predominantly to the exceedance of Cd trigger levels in the Food Standards Australia and New Zealand (FSANZ).
However, recent testing on market sized oysters farmed on long line trials at Pirlangimpi on Tiwi Islands have not shown high heavy metal concentrations and complied with the FSANZ. This suggests that Cd exceedance may not be an issue in all locations. We propose a multi-location survey of blacklip oyster (Saccostrea echinata) heavy metal concentrations across the NT to identify the best locations for commercialisation of this emerging aquaculture species. With the aim of identifying locations, like Pirlangimpi, that could produce oysters that comply with the Food Standards Australia and New Zealand (FSANZ). The results are needed to inform the development of a NT tropical oyster industry and the establishment of a NT shellfish quality assurance program. Objectives: 1. Map the distribution and concentration of Cadmium in wild blacklip oysters across the Northern Territory. 2. Aboriginal communities better understand the role of shellfish quality assurance programs and the implications of Cadmium on oyster farming. 3. Risks associated with Cadmium are better understood and inform the development of a NT Shellfish Quality Assurance Program. 4. Knowledge is shared and retained through Aboriginal participation in the research project. Read moreRead less
Building Capability In Food Safety In Australian Shellfish
Funder
Fisheries Research and Development Corporation
Funding Amount
$351,147.00
Summary
The Australian Shellfish Quality Assurance Advisory Committee (ASQAAC) is a volunteer organisation comprised of members including state regulators, industry representatives and associated stakeholders including researchers, Safefish, seafood importers and laboratories. As the program sets the requirements for market access in Australia through the Australian Shellfish Quality Assurance Program (ASQAP), and influences industry's ability to grow and further develop our low input, carbon friendly a ....The Australian Shellfish Quality Assurance Advisory Committee (ASQAAC) is a volunteer organisation comprised of members including state regulators, industry representatives and associated stakeholders including researchers, Safefish, seafood importers and laboratories. As the program sets the requirements for market access in Australia through the Australian Shellfish Quality Assurance Program (ASQAP), and influences industry's ability to grow and further develop our low input, carbon friendly and job intensive industry. The ASQAP is best practice, and ASQAAC strives to include safe and appropriately innovative and validated measures to ensure market access is maximised - important for both COVID recovery and future proofing the national shellfish industry. There is an on-going need to provide national guidance on ASQAP interpretation and delivery for our industry that has been highlighted by both the succession needs of the well-established programs in SA, Tas and NSW, and by the emerging shellfish industries in WA, NT, and Qld. The market failure for systemisation and training material and continuous improvement stems from the underpinning knowledge of this topic being niche and highly specialised. In the words of the ASQAAC Chair Phil Baker: “The last decade has seen the exit of significant knowledge and history with long-term regulators being replaced with new recruits to the technically complex shellfish regulatory role. Currently there is little to guide them, particularly as ASQAP has transitioned to an outcome-based program. The vital knowledge required is often learned through experience.” This program aims to take advantage of the pool of experts that do exist in Australia, to the benefit of all existing and developing programs.
Objectives: 1. Understand the training needs to support the continued development of Australian shellfish industries covered by ASQAP 2. Develop technical training modules for regulators and industry in bivalve shellfish food safety risk management 3. Deliver the training modules in a manner that suits both industry and regulators, and supports learning from existing Australian experts and adoption of successful risk management strategies 4. Provide a pathway for longevity of the training Read moreRead less