Practical application of gene silencing: is delivery of long double stranded ribonucleic acid (dsRNA) by plant cells efficient in conferring host resistance to parasitic nematodes? Nematode that attack plants cause $120 billion of crop losses worldwide. Chemicals used for their control are being phased out because of environmental concerns, and natural resistance is limited. The aim of this project is to use Australian IP to develop a new form of resistance to nematodes based on knowledge of th ....Practical application of gene silencing: is delivery of long double stranded ribonucleic acid (dsRNA) by plant cells efficient in conferring host resistance to parasitic nematodes? Nematode that attack plants cause $120 billion of crop losses worldwide. Chemicals used for their control are being phased out because of environmental concerns, and natural resistance is limited. The aim of this project is to use Australian IP to develop a new form of resistance to nematodes based on knowledge of the host-pathogen interactions. A successful outcome could contribute an additional 5-20% increase in crop yields (depending on the crop) through inherent resistance of crops to nematode pests. This would benefit rural communities and the national economy, and could also generate international royalties.Read moreRead less
Field based molecular diagnostics for identification of plant parasitic nematodes. Nematodes are economically important pests of many agricultural and commercially grown plants. We have shown 'proof-of-concept' that plant parasitic nematodes can be identified by protein profiling using MALDI-TOF mass spectroscopy. In this project advanced techniques of proteomics and associated bioinformatics will be used to identify, isolate and characterise proteins that are specific to economically important ....Field based molecular diagnostics for identification of plant parasitic nematodes. Nematodes are economically important pests of many agricultural and commercially grown plants. We have shown 'proof-of-concept' that plant parasitic nematodes can be identified by protein profiling using MALDI-TOF mass spectroscopy. In this project advanced techniques of proteomics and associated bioinformatics will be used to identify, isolate and characterise proteins that are specific to economically important nematode species and races, and to identify diagnostic proteins or epitopes. The diagnostic proteins will be used to generate specific monoclonal antibodies that will be incorporated into immunochemical 'Lateral Flow' devices. These will provide on-site tests to identify nematodes for growers and quarantine services. Read moreRead less
Combinatorial controlled gene expression delivering crops resistant to nematodes. Root-knot nematodes cause US$130 billion crop losses worldwide pa, and at least AUS$ 450 pa in Australia. Current control methods involve fumigation, chemicals (mainly carbamates and organophosphates), natural plant resistance and biological control. The fumigants (eg methyl bromide) are being phased out because they damage the ozone layer, most of the non-fumigants are being banned because of environmental damag ....Combinatorial controlled gene expression delivering crops resistant to nematodes. Root-knot nematodes cause US$130 billion crop losses worldwide pa, and at least AUS$ 450 pa in Australia. Current control methods involve fumigation, chemicals (mainly carbamates and organophosphates), natural plant resistance and biological control. The fumigants (eg methyl bromide) are being phased out because they damage the ozone layer, most of the non-fumigants are being banned because of environmental damage and persistence in groundwater, and biological control has had limited success. These problems are addressed in this project with development of synthetic plant resistance to nematodes, which will benefit horticultural and broadacre farming by reducing pathogen losses and improving quality.Read moreRead less
Feasting on protein? Strategies of organic nitrogen acquisition by plant roots. Crops require large amounts of nitrogen for growth. Application of nitrogen fertiliser enhances yield, but causes off-site nitrogen pollution, a main threat to ecosystem integrity. Most nitrogen in soil occurs as organic complexes that are broken down by soil organism into small compounds, which are taken up roots or lost from the soil. This project will generate fundamental knowledge of how an Australian species and ....Feasting on protein? Strategies of organic nitrogen acquisition by plant roots. Crops require large amounts of nitrogen for growth. Application of nitrogen fertiliser enhances yield, but causes off-site nitrogen pollution, a main threat to ecosystem integrity. Most nitrogen in soil occurs as organic complexes that are broken down by soil organism into small compounds, which are taken up roots or lost from the soil. This project will generate fundamental knowledge of how an Australian species and a crop species with unusual root specialisations access soil organic nitrogen, thus increasing the efficiency of nitrogen use and reducing nitrogen loss. The research employs cutting-edge techniques for sustainable resource use, improved efficiency of crops and farming systems, and preservation of Australia's biodiversity.Read moreRead less
Understanding the control of male germ-line development by the germline-restrictive silencing factor in plants. The world population is currently increasing at an unprecedented rate, with a concomitant requirement to double the food production from the same amount of arable land. To ensure global political and social stability, equitably increasing sustainable food production without compromising environmental integrity remains a major challenge. This proposal investigates the molecular mechanis ....Understanding the control of male germ-line development by the germline-restrictive silencing factor in plants. The world population is currently increasing at an unprecedented rate, with a concomitant requirement to double the food production from the same amount of arable land. To ensure global political and social stability, equitably increasing sustainable food production without compromising environmental integrity remains a major challenge. This proposal investigates the molecular mechanisms underlying male germ line initiation and development in plants. Switching off male gamete development in some crop plants will create male sterile lines, which, when crossed with genetically distinct lines, will have the potential to produce hybrids that yield 20-30 percent more crop without additional inputs. Read moreRead less
Epigenetic programming of plant sperm cells. Most of the grains and seeds that form the world's food supply are the result of the successful functioning of sperm and egg cells during fertilisation. This proposal aims to investigate the nature of sperm cell genome programming in plants and unravel molecular processes that give these cells their unique identity. This innovative and challenging research will also provide an excellent opportunity for training the next generation of scientists. The o ....Epigenetic programming of plant sperm cells. Most of the grains and seeds that form the world's food supply are the result of the successful functioning of sperm and egg cells during fertilisation. This proposal aims to investigate the nature of sperm cell genome programming in plants and unravel molecular processes that give these cells their unique identity. This innovative and challenging research will also provide an excellent opportunity for training the next generation of scientists. The outcomes of this proposal will enhance Australia's international lead in this field and will pave the way toward the development of new approaches for sustaining and enhancing crop productivity under changing environmental conditions. Read moreRead less
Does plant phosphorus economy determine ecological status in biodiverse Australian communities? The phosphorus (P)-impoverished nature of the soils of south-western Australia has allowed the evolution of many plant species that are amazingly efficient at acquiring and utilising P. Among the most P efficient are species of the Proteaceae, which produce cluster roots. We will explore why less efficient species can co-occur with the very efficient Proteaceae. A deep and thorough understanding of th ....Does plant phosphorus economy determine ecological status in biodiverse Australian communities? The phosphorus (P)-impoverished nature of the soils of south-western Australia has allowed the evolution of many plant species that are amazingly efficient at acquiring and utilising P. Among the most P efficient are species of the Proteaceae, which produce cluster roots. We will explore why less efficient species can co-occur with the very efficient Proteaceae. A deep and thorough understanding of the mechanism determining P efficiency of these species will contribute significantly to our basic understanding of P nutrition in plants and to our understanding of the P-impoverished ecosystems of the southwest. It will also assist in developing crops that are more efficient at acquiring and/or utilising P.Read moreRead less
Benign strategies to engineer nematode resistance in plant crops. Applications to other plant pests. Control of plant pests relies on the heavy use of chemical insecticides that cause an extraordinary impact on the environment. Some insect pests have been controlled by the production of toxins (like BT) by the plant. We will combine newly discovered RNA interference and genomics methods to develop innovative solutions to nematode resistance and insect control. Our methods can be tailored to any ....Benign strategies to engineer nematode resistance in plant crops. Applications to other plant pests. Control of plant pests relies on the heavy use of chemical insecticides that cause an extraordinary impact on the environment. Some insect pests have been controlled by the production of toxins (like BT) by the plant. We will combine newly discovered RNA interference and genomics methods to develop innovative solutions to nematode resistance and insect control. Our methods can be tailored to any pest with wide or narrow spectrum of action and does not require the production of toxins by the plant. The novelty of our approach will generate a large amount of intellectual property.Read moreRead less
The role of plant hormones in arbuscular mycorrhizal symbiosis. The vast majority of plant species can form a beneficial symbiosis with specialised soil fungi, an association that can enhance the uptake of nutrients from the soil, improve tolerance to drought and disease and minimise soil erosion. An understanding of how plants establish and regulate this important symbiosis has the potential to contribute to the development of productive and sustainable farming systems by making efficient use o ....The role of plant hormones in arbuscular mycorrhizal symbiosis. The vast majority of plant species can form a beneficial symbiosis with specialised soil fungi, an association that can enhance the uptake of nutrients from the soil, improve tolerance to drought and disease and minimise soil erosion. An understanding of how plants establish and regulate this important symbiosis has the potential to contribute to the development of productive and sustainable farming systems by making efficient use of the limited water resources, reducing soil erosion, reducing reliance on pesticides and fertilisers and producing more nutritious fruits, vegetables and grains.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0230245
Funder
Australian Research Council
Funding Amount
$600,000.00
Summary
Joint controlled environment facility for research and development in plant biotechnolgy in Western Australia. The aim of this proposal is to establish a high quality, controlled-environment growth facility for plant research in Perth, jointly managed by Murdoch University and the University of Western Australia. This facility is needed urgently to support current research and new initiatives in plant molecular biology and biotechnology. It will focus on the molecular bases of plant growth and ....Joint controlled environment facility for research and development in plant biotechnolgy in Western Australia. The aim of this proposal is to establish a high quality, controlled-environment growth facility for plant research in Perth, jointly managed by Murdoch University and the University of Western Australia. This facility is needed urgently to support current research and new initiatives in plant molecular biology and biotechnology. It will focus on the molecular bases of plant growth and defence against pathogens. Outcomes include enhancement of WA plant research and its application to improved agricultural production that will benefit rural industries and promote exports. It will also facilitate postgraduate training in plant biotechnology and enhance career prospects of graduates.Read moreRead less