Combating subsoil acidity for sustainable production through managing plant cation-anion uptake. At least 50 million hectares of topsoil and 23 million ha of subsoil of Australian land are presently affected by acidity, with this area expanding due to the ongoing processes of acidification. Surface liming is ineffective in neutralizing subsoil acidity. The project will develop an innovative method that stimulates root proliferation and ameliorates subsoil acidity through manipulating the balance ....Combating subsoil acidity for sustainable production through managing plant cation-anion uptake. At least 50 million hectares of topsoil and 23 million ha of subsoil of Australian land are presently affected by acidity, with this area expanding due to the ongoing processes of acidification. Surface liming is ineffective in neutralizing subsoil acidity. The project will develop an innovative method that stimulates root proliferation and ameliorates subsoil acidity through manipulating the balance of nutrient uptake by plants. The effects of the developed method on nutrient use efficiency and leaching loss, and crop yields will be quantified under different soil types and climatic conditions.Read moreRead less
Synthesis of substrate analogues for probing catalytic mechanisms and specificity of enzymes involved in the metabolism of plant polysaccharides. The project is aimed at strengthening collaborations between research groups in Adelaide and France, with the specific objective of synthesizing substrate analogues as probes of enzymatic mechanisms and substrate specificity in polysaccharide hydrolases and synthases of barley. The chemical expertise resides in France, while the enzymatic work will be ....Synthesis of substrate analogues for probing catalytic mechanisms and specificity of enzymes involved in the metabolism of plant polysaccharides. The project is aimed at strengthening collaborations between research groups in Adelaide and France, with the specific objective of synthesizing substrate analogues as probes of enzymatic mechanisms and substrate specificity in polysaccharide hydrolases and synthases of barley. The chemical expertise resides in France, while the enzymatic work will be conducted largely in Australia. Exchange of research staff, particularly at the postgraduate student and research associate levels, is considered essential to capture the benefits of the complementary expertise and to extend an existing international collaboration. The target enzymes are of central importance in cell wall metabolism during development of higher plants.Read moreRead less
Molecular mechanisms of catalysis and the basis of substrate specificity in polysaccharide hydrolases. Reaction intermediates along hydrolytic pathways and molecular determinants of substrate specificity of barley B-glucan exo- and endohydrolases will be defined using crystallographic and kinetic analyses. These enzymes are of central importance in cell wall metabolism during development of higher plants, and in plant-pathogen interactions. Realization of the project objectives will not only pro ....Molecular mechanisms of catalysis and the basis of substrate specificity in polysaccharide hydrolases. Reaction intermediates along hydrolytic pathways and molecular determinants of substrate specificity of barley B-glucan exo- and endohydrolases will be defined using crystallographic and kinetic analyses. These enzymes are of central importance in cell wall metabolism during development of higher plants, and in plant-pathogen interactions. Realization of the project objectives will not only provide fundamental information on catalytic mechanisms, but will also provide opportunities to manipulate enzyme specificity. Further, site-directed mutagenesis of the enzymes will be used to generate glycosynthases, which will be evaluated for their ability to synthesise novel oligosaccharide and polysaccharide products, some of which might show immunomodulating activity.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE150100837
Funder
Australian Research Council
Funding Amount
$354,000.00
Summary
Engineering enzymes controlling plant polysaccharide properties. This project will aim to use data to define how the synthesis and interconversion of nucleotide sugars is regulated and how this controls the properties of arabinoxylan in economically important plants. Dietary consumption of arabinoxylan reduces chronic diseases. Additionally, the attributes of arabinoxylan influence the cost of processing plant biomass. However, genetic control of the properties of the plant polysaccharide arabin ....Engineering enzymes controlling plant polysaccharide properties. This project will aim to use data to define how the synthesis and interconversion of nucleotide sugars is regulated and how this controls the properties of arabinoxylan in economically important plants. Dietary consumption of arabinoxylan reduces chronic diseases. Additionally, the attributes of arabinoxylan influence the cost of processing plant biomass. However, genetic control of the properties of the plant polysaccharide arabinoxylan is unresolved. A major control point in the partitioning of carbon from photosynthesis into arabinoxylan is the activity of sugar nucleotide interconverting enzymes. To characterise these enzymes, genomic, glycomic and enzyme kinetic data will be combined and the target enzymes will be modified in transgenic plants.Read moreRead less
Improving crops from the ground up: genetic solutions to optimise roots. This project aims to develop future crops with optimised root systems by overcoming genetic constraints that currently restrict their potential. Exploiting advances in genomics, transcriptomics, epigenomics and genome editing, this project expects to advance understanding of the biology and genetic controls of root development and responses to concurrent stressors, including drought, nutrient deficiency and soil-borne disea ....Improving crops from the ground up: genetic solutions to optimise roots. This project aims to develop future crops with optimised root systems by overcoming genetic constraints that currently restrict their potential. Exploiting advances in genomics, transcriptomics, epigenomics and genome editing, this project expects to advance understanding of the biology and genetic controls of root development and responses to concurrent stressors, including drought, nutrient deficiency and soil-borne disease. It is anticipated that project outcomes will support the development of crops equipped with novel root traits, enhancing resource-use efficiency and yield stability amid climate variability. This globally relevant research is designed to benefit the sustainability and profitability of the Australian grains industry.Read moreRead less
New strategies for reducing the concentrations of arsenic and cadmium in crop plants. The research is directed at reducing the concentrations in crops of cadmium and arsenic, two elements that accumulate in humans and can have a range of toxic effects. The results will have widespread implications for improving health in Australia, but are expected to have an even greater impact on populations in parts of Asia where contamination of soil and water by these elements is most severe. The project wi ....New strategies for reducing the concentrations of arsenic and cadmium in crop plants. The research is directed at reducing the concentrations in crops of cadmium and arsenic, two elements that accumulate in humans and can have a range of toxic effects. The results will have widespread implications for improving health in Australia, but are expected to have an even greater impact on populations in parts of Asia where contamination of soil and water by these elements is most severe. The project will train two junior scientists and foster scientific links with China. Read moreRead less