Expression profiling of giant cells induced in host plant roots by root-knot nematodes. Root-knot nematodes cause crop losses of over $400 million per annum in Australia. Control by toxic chemical nematicodes is expensive and can pollute groundwater. Benefits from this research for the Australian community are: (i) it will ensure that Australian researchers stay at the forefront of research in plant nematology, (ii) it provides significant local and international linkages that will stimulate res ....Expression profiling of giant cells induced in host plant roots by root-knot nematodes. Root-knot nematodes cause crop losses of over $400 million per annum in Australia. Control by toxic chemical nematicodes is expensive and can pollute groundwater. Benefits from this research for the Australian community are: (i) it will ensure that Australian researchers stay at the forefront of research in plant nematology, (ii) it provides significant local and international linkages that will stimulate research outputs, and (iii) new knowledge will be generated on how plants respond to attack by nematodes - this will generate new intellectual property, leading to better control methods and reduced costs that will support rural communities, and reduce environmental pollution.Read moreRead less
A novel link between plant pathogen defence and DNA repair capability. Plants and plant-based industries are essential for the provision of food, clothing and building materials and underpin the economies of rural communities. Plant yield and quality and the biodiversity of natural systems are dramatically reduced by disease. The fundamental knowledge gained from our research will enable manipulation of the factors that enhance disease resistance resulting in a significant benefit to Australian ....A novel link between plant pathogen defence and DNA repair capability. Plants and plant-based industries are essential for the provision of food, clothing and building materials and underpin the economies of rural communities. Plant yield and quality and the biodiversity of natural systems are dramatically reduced by disease. The fundamental knowledge gained from our research will enable manipulation of the factors that enhance disease resistance resulting in a significant benefit to Australian agriculture and protection of our natural resources. The current reliance for disease control on chemicals that damage the environment will be reduced and our research will contribute directly to the provision of cheaper, simpler and more effective methods of control.
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Co-evolution of the host pathogen interaction between Leptosphaeria maculans and Brassica species. Brassica canola is Australia's third largest export crop, producing 13% of the world's canola oil. However, blackleg disease, caused by the fungus Leptospheria maculans leads to annual yield losses of 15%, with 100% loss associated with breakdown of resistance. International investment has provided novel genome resources for Brassica and L. maculans. Applying these resources to understand the co-ev ....Co-evolution of the host pathogen interaction between Leptosphaeria maculans and Brassica species. Brassica canola is Australia's third largest export crop, producing 13% of the world's canola oil. However, blackleg disease, caused by the fungus Leptospheria maculans leads to annual yield losses of 15%, with 100% loss associated with breakdown of resistance. International investment has provided novel genome resources for Brassica and L. maculans. Applying these resources to understand the co-evolution of this plant-fungal interaction could prevent the current boom-bust cycle of canola production in Australia. This study will also provide a model and knowledge base for applications in other species, leading to enhanced crops with increased plant protection and robust, reliable productivity.Read moreRead less
Pathogen recognition and plant-defence activation by a novel Fusarium wilt-resistance protein from tomato. The devastating effects of Fusarium wilt disease of tomato is a threat to one of Australia's most economically important horticultural crops. Resistant tomato varieties offer the most effective means of control but the fundamental mechanisms underlying this resistance are yet to be understood. This research will increase our understanding of resistance to Fusarium wilt disease. The knowledg ....Pathogen recognition and plant-defence activation by a novel Fusarium wilt-resistance protein from tomato. The devastating effects of Fusarium wilt disease of tomato is a threat to one of Australia's most economically important horticultural crops. Resistant tomato varieties offer the most effective means of control but the fundamental mechanisms underlying this resistance are yet to be understood. This research will increase our understanding of resistance to Fusarium wilt disease. The knowledge gained will assist in the development of new robust, sustainable approaches to disease control, as well as the development of pre-emptive strategies to avert major outbreaks, which will ensure reliable productivity and minimal economic losses into the future.Read moreRead less
Control of sucrose source/sink status by the plant immune system. This project aims to investigate the roles of plant malectin receptor kinases in pathogen recognition and response. The project will utilise molecular and cell biological tools to detect complex formation and changes in sugar import into cells. Expected outcomes of this project include a better understanding of how pathogens manipulate plant cells to their benefit, and greater knowledge of which host molecules participate in this ....Control of sucrose source/sink status by the plant immune system. This project aims to investigate the roles of plant malectin receptor kinases in pathogen recognition and response. The project will utilise molecular and cell biological tools to detect complex formation and changes in sugar import into cells. Expected outcomes of this project include a better understanding of how pathogens manipulate plant cells to their benefit, and greater knowledge of which host molecules participate in this process. This should provide significant benefits such as a new theoretical basis to engineer crop plants for resistance against devastating diseases.Read moreRead less
Engineering and expression of recombinant antibodies that interact with plant pathogenic phytoplasma membrane proteins - a model for phytoplasma disease management. . In Australia phytoplasmas are associated with serious diseases including papaya dieback and strawberry lethal yellows. Phytoplasmas cannot be cultured so there is a paucity of knowledge at the genomic level. We have identified the ABC Transporter and FtsH genes and expressed these phytoplasma membrane proteins (MP) in E. coli . We ....Engineering and expression of recombinant antibodies that interact with plant pathogenic phytoplasma membrane proteins - a model for phytoplasma disease management. . In Australia phytoplasmas are associated with serious diseases including papaya dieback and strawberry lethal yellows. Phytoplasmas cannot be cultured so there is a paucity of knowledge at the genomic level. We have identified the ABC Transporter and FtsH genes and expressed these phytoplasma membrane proteins (MP) in E. coli . We will generate antibody fragments that bind these MP and express them in tomato. We will analyse antibody expression in tomato and study their association with the phytoplasma membrane. Transgenic plants will be inoculated with phytoplasma and monitored for resistance. This work represents a major step towards engineering resistance to phytoplasmas.Read moreRead less
FUNCTIONAL GENOMICS OF PLANT MITOCHONDRIA: THEIR ROLE IN DEVELOPMENT, OXIDATIVE STRESS AND PLANT DEFENSE. Crops encounter many situations in their environment which place them under stress. Reactive oxygen molecules produced in these situations act as messengers to trigger defence mechanisms but also cause cellular damage. Mitochondria are the subcellular compartments involved in energy production and are essential for plant development and growth. However, they also have been implicated in th ....FUNCTIONAL GENOMICS OF PLANT MITOCHONDRIA: THEIR ROLE IN DEVELOPMENT, OXIDATIVE STRESS AND PLANT DEFENSE. Crops encounter many situations in their environment which place them under stress. Reactive oxygen molecules produced in these situations act as messengers to trigger defence mechanisms but also cause cellular damage. Mitochondria are the subcellular compartments involved in energy production and are essential for plant development and growth. However, they also have been implicated in the response of plants to stress and pathogen attack, and in production of reactive oxygen molecules. This proposal seeks to investigate how mitochondria are involved in these processes, using the latest plant genome information. Potential outcomes include crops better able to cope with environmental stress.Read moreRead less
Targeting of lipid transfer proteins and other secreted plant defence proteins by pathogen effectors. Australia depends on plant products for much of its export income and plant diseases are a continual threat to these earnings. New insights into plant disease resistance mechanisms will provide new opportunities to find solutions to disease problems. Often this involves transfer of knowledge gained in simple model plant systems to more complicated crop plant systems. Work on the role of lipid tr ....Targeting of lipid transfer proteins and other secreted plant defence proteins by pathogen effectors. Australia depends on plant products for much of its export income and plant diseases are a continual threat to these earnings. New insights into plant disease resistance mechanisms will provide new opportunities to find solutions to disease problems. Often this involves transfer of knowledge gained in simple model plant systems to more complicated crop plant systems. Work on the role of lipid transfer proteins in the tomato model system will advance our knowledge of fundamental disease and disease resistance processes and ultimately enable technological innovations to improve disease resistance, reduce pesticide usage and lower crop production costs.Read moreRead less
Plant microRNA systems: investigating slicing versus translational repression and the development of an anti-viral defence mechanism. The ability to specifically switch off genes has revolutionised biotechnological approaches, from fighting diseases to developing superior crops, however the underlying mechanism is poorly understood, limiting its application in practice. Outcomes from this project will provide such knowledge, allowing its precise use in diverse applications.