Practical application of gene silencing: is delivery of long double stranded ribonucleic acid (dsRNA) by plant cells efficient in conferring host resistance to parasitic nematodes? Nematode that attack plants cause $120 billion of crop losses worldwide. Chemicals used for their control are being phased out because of environmental concerns, and natural resistance is limited. The aim of this project is to use Australian IP to develop a new form of resistance to nematodes based on knowledge of th ....Practical application of gene silencing: is delivery of long double stranded ribonucleic acid (dsRNA) by plant cells efficient in conferring host resistance to parasitic nematodes? Nematode that attack plants cause $120 billion of crop losses worldwide. Chemicals used for their control are being phased out because of environmental concerns, and natural resistance is limited. The aim of this project is to use Australian IP to develop a new form of resistance to nematodes based on knowledge of the host-pathogen interactions. A successful outcome could contribute an additional 5-20% increase in crop yields (depending on the crop) through inherent resistance of crops to nematode pests. This would benefit rural communities and the national economy, and could also generate international royalties.Read moreRead less
Field based molecular diagnostics for identification of plant parasitic nematodes. Nematodes are economically important pests of many agricultural and commercially grown plants. We have shown 'proof-of-concept' that plant parasitic nematodes can be identified by protein profiling using MALDI-TOF mass spectroscopy. In this project advanced techniques of proteomics and associated bioinformatics will be used to identify, isolate and characterise proteins that are specific to economically important ....Field based molecular diagnostics for identification of plant parasitic nematodes. Nematodes are economically important pests of many agricultural and commercially grown plants. We have shown 'proof-of-concept' that plant parasitic nematodes can be identified by protein profiling using MALDI-TOF mass spectroscopy. In this project advanced techniques of proteomics and associated bioinformatics will be used to identify, isolate and characterise proteins that are specific to economically important nematode species and races, and to identify diagnostic proteins or epitopes. The diagnostic proteins will be used to generate specific monoclonal antibodies that will be incorporated into immunochemical 'Lateral Flow' devices. These will provide on-site tests to identify nematodes for growers and quarantine services. Read moreRead less
Combinatorial controlled gene expression delivering crops resistant to nematodes. Root-knot nematodes cause US$130 billion crop losses worldwide pa, and at least AUS$ 450 pa in Australia. Current control methods involve fumigation, chemicals (mainly carbamates and organophosphates), natural plant resistance and biological control. The fumigants (eg methyl bromide) are being phased out because they damage the ozone layer, most of the non-fumigants are being banned because of environmental damag ....Combinatorial controlled gene expression delivering crops resistant to nematodes. Root-knot nematodes cause US$130 billion crop losses worldwide pa, and at least AUS$ 450 pa in Australia. Current control methods involve fumigation, chemicals (mainly carbamates and organophosphates), natural plant resistance and biological control. The fumigants (eg methyl bromide) are being phased out because they damage the ozone layer, most of the non-fumigants are being banned because of environmental damage and persistence in groundwater, and biological control has had limited success. These problems are addressed in this project with development of synthetic plant resistance to nematodes, which will benefit horticultural and broadacre farming by reducing pathogen losses and improving quality.Read moreRead less
Does plant phosphorus economy determine ecological status in biodiverse Australian communities? The phosphorus (P)-impoverished nature of the soils of south-western Australia has allowed the evolution of many plant species that are amazingly efficient at acquiring and utilising P. Among the most P efficient are species of the Proteaceae, which produce cluster roots. We will explore why less efficient species can co-occur with the very efficient Proteaceae. A deep and thorough understanding of th ....Does plant phosphorus economy determine ecological status in biodiverse Australian communities? The phosphorus (P)-impoverished nature of the soils of south-western Australia has allowed the evolution of many plant species that are amazingly efficient at acquiring and utilising P. Among the most P efficient are species of the Proteaceae, which produce cluster roots. We will explore why less efficient species can co-occur with the very efficient Proteaceae. A deep and thorough understanding of the mechanism determining P efficiency of these species will contribute significantly to our basic understanding of P nutrition in plants and to our understanding of the P-impoverished ecosystems of the southwest. It will also assist in developing crops that are more efficient at acquiring and/or utilising P.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0230245
Funder
Australian Research Council
Funding Amount
$600,000.00
Summary
Joint controlled environment facility for research and development in plant biotechnolgy in Western Australia. The aim of this proposal is to establish a high quality, controlled-environment growth facility for plant research in Perth, jointly managed by Murdoch University and the University of Western Australia. This facility is needed urgently to support current research and new initiatives in plant molecular biology and biotechnology. It will focus on the molecular bases of plant growth and ....Joint controlled environment facility for research and development in plant biotechnolgy in Western Australia. The aim of this proposal is to establish a high quality, controlled-environment growth facility for plant research in Perth, jointly managed by Murdoch University and the University of Western Australia. This facility is needed urgently to support current research and new initiatives in plant molecular biology and biotechnology. It will focus on the molecular bases of plant growth and defence against pathogens. Outcomes include enhancement of WA plant research and its application to improved agricultural production that will benefit rural industries and promote exports. It will also facilitate postgraduate training in plant biotechnology and enhance career prospects of graduates.Read moreRead less
Carboxylate exudation and phosphate nutrition in Hakea prostrata (Proteaecea). Nonmycorrhizal Proteaceae are very successful in acquiring phosphate from nutrient-impoverished soils; their cluster roots account for this. They are also extremely sensitive to phosphate toxicity. We will elucidate aspects of production and release of carboxylates that are associated with functioning of cluster roots, using Hakea prostrata (Proteaceae) as a model. Types and rates of exudation by cluster roots, as in ....Carboxylate exudation and phosphate nutrition in Hakea prostrata (Proteaecea). Nonmycorrhizal Proteaceae are very successful in acquiring phosphate from nutrient-impoverished soils; their cluster roots account for this. They are also extremely sensitive to phosphate toxicity. We will elucidate aspects of production and release of carboxylates that are associated with functioning of cluster roots, using Hakea prostrata (Proteaceae) as a model. Types and rates of exudation by cluster roots, as influenced by development and environmental signals, will be assessed. Our findings will provide key information on adaptive mechanisms associated with both phosphate acquisition from phosphate-fixing soils and phosphate toxicity.Read moreRead less
Expression profiling of giant cells induced in host plant roots by root-knot nematodes. Root-knot nematodes cause crop losses of over $400 million per annum in Australia. Control by toxic chemical nematicodes is expensive and can pollute groundwater. Benefits from this research for the Australian community are: (i) it will ensure that Australian researchers stay at the forefront of research in plant nematology, (ii) it provides significant local and international linkages that will stimulate res ....Expression profiling of giant cells induced in host plant roots by root-knot nematodes. Root-knot nematodes cause crop losses of over $400 million per annum in Australia. Control by toxic chemical nematicodes is expensive and can pollute groundwater. Benefits from this research for the Australian community are: (i) it will ensure that Australian researchers stay at the forefront of research in plant nematology, (ii) it provides significant local and international linkages that will stimulate research outputs, and (iii) new knowledge will be generated on how plants respond to attack by nematodes - this will generate new intellectual property, leading to better control methods and reduced costs that will support rural communities, and reduce environmental pollution.Read moreRead less
Race status, sources of resistance and mechanisms of resistance to Peronospora parasitica, a major threat to oilseed Brassica production in Australia. Through successful identification of mechanisms and molecular characterisation of resistance to Peronospora parasitica races and the identification of sources of host resistance against these races, breeders, for the first time, will be able to develop cultivars with resistance against the full spectrum of P. parasitica races occurring across sout ....Race status, sources of resistance and mechanisms of resistance to Peronospora parasitica, a major threat to oilseed Brassica production in Australia. Through successful identification of mechanisms and molecular characterisation of resistance to Peronospora parasitica races and the identification of sources of host resistance against these races, breeders, for the first time, will be able to develop cultivars with resistance against the full spectrum of P. parasitica races occurring across southern Australia. Benefits include prevention of severe losses in canola from downy mildew, and more viable and sustainable production with less reliance upon fungicides. This research addresses the National Research Priority 'An Environmentally Sustainable Australia' and the Priority Goal of 'Transforming existing industries', and will particularly benefit southern Australian rural communities.Read moreRead less
A new approach to control of plant parasitic nematodes. Plant parasitic nematodes are the fourth most important plant pathogens worldwide. The aim of this project is to develop ?proof-of-concept? of a new strategy for nematode control, based on a synthetic ?cell death? resistance gene, in which tight specificity of expression of the resistance gene is provided using two gene promoters. The work focusses on finding the best combination of promoters which are switched on by infection of plants w ....A new approach to control of plant parasitic nematodes. Plant parasitic nematodes are the fourth most important plant pathogens worldwide. The aim of this project is to develop ?proof-of-concept? of a new strategy for nematode control, based on a synthetic ?cell death? resistance gene, in which tight specificity of expression of the resistance gene is provided using two gene promoters. The work focusses on finding the best combination of promoters which are switched on by infection of plants with nematodes. It is based on an Australian patent which has wide applicability in plant biotechnology. Nematode control will benefit horticultural and broadacre farming by reducing pathogen losses and improving quality.Read moreRead less
Special Research Initiatives - Grant ID: SR0354715
Funder
Australian Research Council
Funding Amount
$40,000.00
Summary
The Australian Plant Nutriomics Network. The Australian Plant Nutriomics Network will link Australian scientists investigating aspects of the plant nutriome - the summation of processes that deliver nutrients and water from soil to plants. The network will establish a coordinated approach to understanding genes, proteins and metabolites involved in element acquisition and how their functions are linked to soil conditions to maximise food quality and overcome soil environmental challenges. Inter ....The Australian Plant Nutriomics Network. The Australian Plant Nutriomics Network will link Australian scientists investigating aspects of the plant nutriome - the summation of processes that deliver nutrients and water from soil to plants. The network will establish a coordinated approach to understanding genes, proteins and metabolites involved in element acquisition and how their functions are linked to soil conditions to maximise food quality and overcome soil environmental challenges. International articulation will ensure information exchange and enhance postgraduate and postdoctoral training by reciprocal visits and focused workshops. A major goal will be a strategy to integrate research using a complex systems approach to problems.Read moreRead less