A high-through-put method for unlocking the mitochondrial genomes of significant pathogens. The national/community benefits of this research are: (1) to develop a long-term, high quality scientific and technological program contributing to national objectives, including the maintenance of a strong capability in basic research, the development of new scientific concepts and the enhancement of international collaborative links; (2) to strengthen the links between basic and applied research; (3) to ....A high-through-put method for unlocking the mitochondrial genomes of significant pathogens. The national/community benefits of this research are: (1) to develop a long-term, high quality scientific and technological program contributing to national objectives, including the maintenance of a strong capability in basic research, the development of new scientific concepts and the enhancement of international collaborative links; (2) to strengthen the links between basic and applied research; (3) to develop excellence in research by promoting collaborative research, resulting in a more efficient use of resources in a national and international context; (4) to enhance the skills-base in biology and biotechnology; and (5) to substantially increase global visibility through quality research, leading to an increased investment in Australian science.Read moreRead less
Sex, parthenogenesis and adaptation: a novel laboratory 'natural selection' experiment testing the adaptive significance of sexual and asexual reproduction. One of the greatest challenges for evolutionary biology is explaining the widespread occurrence of sexual reproduction. Many theoretical models show some inherent benefits of sex, however these models make assumptions that little or no empirical data exists for. Using a novel phenomenon found in the parasitoid wasp genus Trichogramma, I will ....Sex, parthenogenesis and adaptation: a novel laboratory 'natural selection' experiment testing the adaptive significance of sexual and asexual reproduction. One of the greatest challenges for evolutionary biology is explaining the widespread occurrence of sexual reproduction. Many theoretical models show some inherent benefits of sex, however these models make assumptions that little or no empirical data exists for. Using a novel phenomenon found in the parasitoid wasp genus Trichogramma, I will investigate the adaptive significance of sexual and asexual reproduction in a laboratory natural selection experiment and therefore directly test the theory for the evolution and maintenance of sex. This will be the first such test in a higher organism.Read moreRead less
Breaking selective constraints: is Hsp90 a capacitor of evolutionary change? Gene action and expression can be modulated by genetic mechanisms. If a general mechanism controlling gene expression exists that can be easily manipulated it has the potential to allow animal and plant breeders to quickly produce and select for new characters of agricultural importance without relying on transgenics and long-term breeding programs. It would also assist in the development of tools for identifying and de ....Breaking selective constraints: is Hsp90 a capacitor of evolutionary change? Gene action and expression can be modulated by genetic mechanisms. If a general mechanism controlling gene expression exists that can be easily manipulated it has the potential to allow animal and plant breeders to quickly produce and select for new characters of agricultural importance without relying on transgenics and long-term breeding programs. It would also assist in the development of tools for identifying and detecting genes that increase susceptibility to human diseases, such as cancer, that are only expressed under certain conditions environmental. I intend to determine whether the protein Hsp90 represents a general mechanism controlling gene expression. Read moreRead less
The Evolution and Diversification of Apicomplexan Cell Invasion Mechanisms. Insights gained through this project, about the mechanisms of cell invasion in Apicomplexan parasites, will have far reaching implications for a number of parasites of great significance to humans and animals. Since host cell invasion is a key step in the parasite lifecycle, proteins identified here will be prime targets for novel drugs that prevent invasion or antigens that can be used as vaccines. This will be importan ....The Evolution and Diversification of Apicomplexan Cell Invasion Mechanisms. Insights gained through this project, about the mechanisms of cell invasion in Apicomplexan parasites, will have far reaching implications for a number of parasites of great significance to humans and animals. Since host cell invasion is a key step in the parasite lifecycle, proteins identified here will be prime targets for novel drugs that prevent invasion or antigens that can be used as vaccines. This will be important for developing new control strategies for diseases of global significance such as malaria or toxoplasmosis, as well as those of national importance to the food industry of Australia, including diseases like babesiosis and coccidiosis that cause significant economic loss to the livestock and poultry industries each year.Read moreRead less
The success and rapid evolution of invasive species: resolving a genetic paradox. Invasive species have an enormous economic impact on trade, agriculture, aquaculture, fisheries and eco-tourism in Australia. In order to negate such detrimental impacts, integrated strategies need to be formulated to effectively control and eradicate introduced species. Our limited knowledge of the factors underlying the success of invasive species has hampered such efforts. A detailed understanding of the invasio ....The success and rapid evolution of invasive species: resolving a genetic paradox. Invasive species have an enormous economic impact on trade, agriculture, aquaculture, fisheries and eco-tourism in Australia. In order to negate such detrimental impacts, integrated strategies need to be formulated to effectively control and eradicate introduced species. Our limited knowledge of the factors underlying the success of invasive species has hampered such efforts. A detailed understanding of the invasion dynamics of introduced species is essential for identifying potential weaknesses that can be exploited in management strategies. My research will examine the invasion dynamics of a successful invasive species, the delicate skink, providing information directly relevant to the management of introduced species in Australia.Read moreRead less
Genetic variation without sex: frequency-dependent selection and the maintenance of variation in asexual organisms. The proposed research will gain insight into one of the most fundamental yet puzzling questions in evolutionary biology and determine the generality of frequency-dependent selection as a mechanism for maintaining variation in asexual organisms. The results will have broad appeal, both internationally and nationally, and will also provide insight into why most species reproduce sexu ....Genetic variation without sex: frequency-dependent selection and the maintenance of variation in asexual organisms. The proposed research will gain insight into one of the most fundamental yet puzzling questions in evolutionary biology and determine the generality of frequency-dependent selection as a mechanism for maintaining variation in asexual organisms. The results will have broad appeal, both internationally and nationally, and will also provide insight into why most species reproduce sexually. The results will also help in the development of two asexual species as environmental indicators by linking clones with specific environmental changes. These indicator clones could then be adopted by natural resource managers to monitor environmental pollution and contamination.Read moreRead less
Using comparative genomics to identify genes responsible for adaptation to environmental toxins. The US National Human Genome Research Institute has committed to sequencing the genomes of ten different Drosophila (fly) species. We will search these genomes, and two others that are already available, for genes that allow flies to cope with environmental toxins found in the plants upon which they feed and breed. These same genes have the potential to degrade many of the insecticides used to con ....Using comparative genomics to identify genes responsible for adaptation to environmental toxins. The US National Human Genome Research Institute has committed to sequencing the genomes of ten different Drosophila (fly) species. We will search these genomes, and two others that are already available, for genes that allow flies to cope with environmental toxins found in the plants upon which they feed and breed. These same genes have the potential to degrade many of the insecticides used to control insect pests. Hence, this research will contribute to ongoing efforts to minimize the threat to agriculture posed by the insecticide resistance that frequently evolves in pest species. Read moreRead less
Colour polymorphisms and speciation: linking macroevolutionary patterns with microevolutionary processes. Polymorphic species with many different colour forms have inspired biological inquiry since Aristotle. This project takes advantage of remarkable variation in male throat coloration in the South Australian tawny dragon lizard to contribute to a fundamental, yet unresolved, question in biology: what are the evolutionary processes generating biodiversity? This research will reinforce Australia ....Colour polymorphisms and speciation: linking macroevolutionary patterns with microevolutionary processes. Polymorphic species with many different colour forms have inspired biological inquiry since Aristotle. This project takes advantage of remarkable variation in male throat coloration in the South Australian tawny dragon lizard to contribute to a fundamental, yet unresolved, question in biology: what are the evolutionary processes generating biodiversity? This research will reinforce Australia's outstanding reputation in the fields of Ecology and Evolution and contribute to the training of internationally competitive postgraduate students. It will also improve our understanding of the biology and genetic diversity of a fascinating Australian reptile and its habitat, within a region of national biodiversity significance.Read moreRead less
A molecular phylogenetic study of the evolution of ecological specialisation in Amphibolurinae agamid lizards. This project will use a comparative approach that combines ecological, morphological and molecular data to investigate evolutionary patterns of ecological specialisation in Australian agamid lizards. Understanding the evolutionary pattern of ecological specialisation, or the directional course of evolution, is an unresolved question in evolutionary biology. Quantitative investigations o ....A molecular phylogenetic study of the evolution of ecological specialisation in Amphibolurinae agamid lizards. This project will use a comparative approach that combines ecological, morphological and molecular data to investigate evolutionary patterns of ecological specialisation in Australian agamid lizards. Understanding the evolutionary pattern of ecological specialisation, or the directional course of evolution, is an unresolved question in evolutionary biology. Quantitative investigations of ecological specialisation are rare because research has been limited by molecular and analytical techniques. Recent advances in population genetics and bio-statistics will allow me to test hypotheses concerning the directionality of evolution. This study will provide a foundation for future research in evolutionary biology and greatly expand our knowledge of agamid biodiversity.Read moreRead less
Evaluating the adaptive potential of organisms to respond to environmental change. The program utilizes technological advances to develop a gene inventory for climatic change adaptation, using, as model system, the vinegar fly from divergent climates along eastern Australia. The inventory will result in new methods for monitoring climatic change impact on populations, and for testing adaptive potential of organisms from threatened habitats. A key hypothesis is that these organisms have restricte ....Evaluating the adaptive potential of organisms to respond to environmental change. The program utilizes technological advances to develop a gene inventory for climatic change adaptation, using, as model system, the vinegar fly from divergent climates along eastern Australia. The inventory will result in new methods for monitoring climatic change impact on populations, and for testing adaptive potential of organisms from threatened habitats. A key hypothesis is that these organisms have restricted genetic options to counter environmental change, increasing extinction risk. The program investigates genetic adaptation to pollutants in midges, a key group for monitoring water health. By assessing evolutionary potential and DNA species markers, biological signatures of aquatic pollutants should result.Read moreRead less