Bioanalytical Microchips Based on Integrated, Application Tailored Monolithic Modules. Microfluidic devices offer substantial advantages over current technology, in terms of speed, cost of analysis, portability, operator simplicity and safety. Integrating multiple analytical processes within a simple and reliable portable device will lead to application in a range of areas, from pharmacology to therapeutic drug monitoring, proteomic and metabolomic screening for disease diagnosis and drug develo ....Bioanalytical Microchips Based on Integrated, Application Tailored Monolithic Modules. Microfluidic devices offer substantial advantages over current technology, in terms of speed, cost of analysis, portability, operator simplicity and safety. Integrating multiple analytical processes within a simple and reliable portable device will lead to application in a range of areas, from pharmacology to therapeutic drug monitoring, proteomic and metabolomic screening for disease diagnosis and drug development, and also for performing clinical diagnostics in a rural area. This will significantly impact on the quality of life of the Nation as a whole, not only due to expedient diagnosis and treatment which has obvious health benefits, but also in the considerable financial benefits that result from early and efficient treatment. Read moreRead less
Enhancing Selectivity and Detection in Miniaturised Analytical Separation Systems. Miniaturisation-compatible stationary phases and detection techniques will be developed for microseparation techniques of capillary electrochromatography and chip-based separation systems, and their analytical applications will be investigated. Replaceable stationary phases (RSP) of chromatographic particles suspended in reversible gels will be created by delivering a liquid RSP to the column followed by in situ ....Enhancing Selectivity and Detection in Miniaturised Analytical Separation Systems. Miniaturisation-compatible stationary phases and detection techniques will be developed for microseparation techniques of capillary electrochromatography and chip-based separation systems, and their analytical applications will be investigated. Replaceable stationary phases (RSP) of chromatographic particles suspended in reversible gels will be created by delivering a liquid RSP to the column followed by in situ immobilisation. This approach offers flexibility in optimising a number of parameters of the column and of its use for sample pre-treatment and preconcentration. Pulsed potentiometric detection will be developed for the abovementioned microseparation techniques, combining the advantages of pulsed amperometric techniques with the more universally responding potentiometric detection.Read moreRead less
Towards Microfluidic-Based Advanced Remote Analysis. The research under this project will establish and systematically develop Advanced Remote Analysis as a new inter-disciplinary area and establish a leadership role for Australia. By addressing pressing needs such as monitoring the environment, remote medical diagnostics, advancing Australian science and technology, or monitoring for traces of explosives, this project falls directly into all four of the National Research Priorities with applica ....Towards Microfluidic-Based Advanced Remote Analysis. The research under this project will establish and systematically develop Advanced Remote Analysis as a new inter-disciplinary area and establish a leadership role for Australia. By addressing pressing needs such as monitoring the environment, remote medical diagnostics, advancing Australian science and technology, or monitoring for traces of explosives, this project falls directly into all four of the National Research Priorities with applications addressing corresponding Priority Goals. Other areas benefiting from the outcomes of this project will be remote monitoring of agricultural production including living species, and a number of other industries such as biotechnology, mineral processing, power generation etc.Read moreRead less
In-line SPE-CE for the direct determination of drugs and metabolites in biological fluids based on porous polymer monoliths. A new approach is proposed for the development of novel micro- and nano-scale solid-phase adsorbent materials. These materials are based on porous polymer monoliths formed in situ within a capillary acting as a mould. The allows the material to be readily placed in a defined position, in a process similar to photo-patterning, and alleviates many of the problems usually as ....In-line SPE-CE for the direct determination of drugs and metabolites in biological fluids based on porous polymer monoliths. A new approach is proposed for the development of novel micro- and nano-scale solid-phase adsorbent materials. These materials are based on porous polymer monoliths formed in situ within a capillary acting as a mould. The allows the material to be readily placed in a defined position, in a process similar to photo-patterning, and alleviates many of the problems usually associated with fabrication and miniaturisation. A simple photo-grafting process, initiated by UV light can be used for the selective chemical modification of these materials. These tailored monoliths can then be used for the in-line coupling of biological sample handling and capillary electrophoresis (CE) for the determination of drugs and related metabolites in biological fluids, thus avoiding time-consuming and costly off-line sample pre-treatment. This will lead to the development of new methods for the rapid determination of drugs, metabolites and other small molecules in clinical samples.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0237784
Funder
Australian Research Council
Funding Amount
$707,000.00
Summary
Hyphenated Separations/Mass Spectrometry Technology for Protein and Natural Product Characterisation. This proposal consolidates the 3 institutions' collaboration on the ACROSS (Australian Centre for Research on Separation Science) initiative, providing fundamental technology for chemical structural analysis of complex samples involving high resolution protein and natural product characterisation. Requested equipment supports complete characterisation of important novel target molecules. GCxGC-T ....Hyphenated Separations/Mass Spectrometry Technology for Protein and Natural Product Characterisation. This proposal consolidates the 3 institutions' collaboration on the ACROSS (Australian Centre for Research on Separation Science) initiative, providing fundamental technology for chemical structural analysis of complex samples involving high resolution protein and natural product characterisation. Requested equipment supports complete characterisation of important novel target molecules. GCxGC-TOFMS technology will validate our newly patented multidimensional separation techniques. Q-TOF-TOFMS technology, novel patented protein prefractionation approaches, and sample handling with high resolution characterisation / identification of new target proteins allows advanced proteomics developments. Proteomics depends critically upon sophisticated MS techniques. These technologies will: ·enhance the capabilities and expertise in these sciences in the SE Australian area; ·ensure the ACROSS initiative achieves internationally competitive research capabilities; ·provide commercial endpoints in fields associated with analysis of proteins, essential oils and other natural (bio)substances.Read moreRead less