The role of the transcription factor Runx2 during mammary gland development and lactation. This proposal will further our understanding of mammary gland development and lactation and the mechanisms controlling mammary cell fate decisions such as differentiation. Regulation of cell fate lies at the core of most aspects of cell biology from normal development to dysfunction such as cancer. The knowledge gleamed from this project also has the potential to make economic gains for Australia by increa ....The role of the transcription factor Runx2 during mammary gland development and lactation. This proposal will further our understanding of mammary gland development and lactation and the mechanisms controlling mammary cell fate decisions such as differentiation. Regulation of cell fate lies at the core of most aspects of cell biology from normal development to dysfunction such as cancer. The knowledge gleamed from this project also has the potential to make economic gains for Australia by increasing the profitability and ensuring the sustainability of both the dairy and meat industries. Better understanding of the mechanisms controlling mammary epithelial cell differentiation should enable augmentation of lactation such as increasing milk protein content, using marker assisted selection (of targets such as Runx2) in cattle.Read moreRead less
Understanding the chemical components that influence the flavour and quality of milk under different pasture management systems. The project will preserve and substantially increase the penetration of Australian milk into the Asian import market for dairy products, which stands at $2.1 billion annually, and is expected to grow at 6.6%per annum over the next 5 years. This outcome can potentially counteract the decline in dairy farm numbers in northern NSW, attract new suppliers to the region, an ....Understanding the chemical components that influence the flavour and quality of milk under different pasture management systems. The project will preserve and substantially increase the penetration of Australian milk into the Asian import market for dairy products, which stands at $2.1 billion annually, and is expected to grow at 6.6%per annum over the next 5 years. This outcome can potentially counteract the decline in dairy farm numbers in northern NSW, attract new suppliers to the region, and stimulate rural community growth and prosperity.Read moreRead less
Cystine flux and hydrogen peroxide breakdown in reuteri group lactobacilli. We have discovered, and aim to fully dissect a novel function of reuteri group lactobacilli. These microbes inhabit the surfaces of the gastrointestinal and reproductive tracts of humans and are also used as probiotics, and in the dairy industry. This function mediates the production and breakdown of large amounts of hydrogen peroxide, cystine and thiols. These are highly bioactive and affect human cells and other bacter ....Cystine flux and hydrogen peroxide breakdown in reuteri group lactobacilli. We have discovered, and aim to fully dissect a novel function of reuteri group lactobacilli. These microbes inhabit the surfaces of the gastrointestinal and reproductive tracts of humans and are also used as probiotics, and in the dairy industry. This function mediates the production and breakdown of large amounts of hydrogen peroxide, cystine and thiols. These are highly bioactive and affect human cells and other bacteria at the surfaces of the gastrointestinal and reproductive tracts, and thiols are also flavourants in dairy products. Our research will inform the rational development of probiotics, the management and treatment of unpleasant conditions such as Crohn's disease and bacterial vaginosis, and innovation in dairy fermentations.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0454052
Funder
Australian Research Council
Funding Amount
$733,595.00
Summary
Tandem Matrix-Assisted Laser Desorption/Ionisation Time-Of-Flight Mass Spectrometer and Robots for High Throughput Proteomics Analysis. This proposal seeks to establish the capacity to perform high-energy tandem mass spectrometry on a high throughput basis, through purchase and coordinated operation of a Matrix-Assisted Laser Desorption/Ionisation - Time of Flight / Time of Flight - Mass Spectrometer and ancillary equipment, to enhance the proteomics expertise, infrastructure and research plans ....Tandem Matrix-Assisted Laser Desorption/Ionisation Time-Of-Flight Mass Spectrometer and Robots for High Throughput Proteomics Analysis. This proposal seeks to establish the capacity to perform high-energy tandem mass spectrometry on a high throughput basis, through purchase and coordinated operation of a Matrix-Assisted Laser Desorption/Ionisation - Time of Flight / Time of Flight - Mass Spectrometer and ancillary equipment, to enhance the proteomics expertise, infrastructure and research plans of a network of institutions from Queensland and New South Wales and their collaborators. Access to such instrumentation is critical to high level achievement in proteomics, a key platform technology for National Research Priorities relating to Frontier Technologies. No comparable instrument currently exists in Australia.Read moreRead less
An investigation into flagellotropic bacteriophage stability and biology. This project aims to understand the capabilities of a type of virus called bacteriophage (phage). Significant economic loss in the food industry has led the USA and Europe to deploy phage to decontaminate food-processing machinery. These phage kill food-spoiling bacteria. This project expects to fill gaps in our knowledge, particularly to assit in choosing phage that are of increased stability and thus more long-lasting fo ....An investigation into flagellotropic bacteriophage stability and biology. This project aims to understand the capabilities of a type of virus called bacteriophage (phage). Significant economic loss in the food industry has led the USA and Europe to deploy phage to decontaminate food-processing machinery. These phage kill food-spoiling bacteria. This project expects to fill gaps in our knowledge, particularly to assit in choosing phage that are of increased stability and thus more long-lasting for deployment in industrial settings, and to inform additives to the phage preparations to increase their potency in killing bacteria. The project should provide significant benefits in training students and staff in methodology for investigating phage for future applications in Australian industry and biotechnology.
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