Development Of Therapeutically Useful Human Artificial Chromosomes For Gene Delivery And Optimal Gene Expression
Funder
National Health and Medical Research Council
Funding Amount
$496,986.00
Summary
Gene therapy is an exciting new form of treatment for genetic disorders aimed at providing long-term correction of the problems at source - namely the affected gene. The biggest technical hurdle facing gene therapy is to be able to deliver the therapeutic genes efficiently and safely into patient cells. Many gene therapy protocols are currently being trialled clinically. These protocols, based mostly on the use of attenuated viruses to deliver the genes, carry potential risks to the patients in ....Gene therapy is an exciting new form of treatment for genetic disorders aimed at providing long-term correction of the problems at source - namely the affected gene. The biggest technical hurdle facing gene therapy is to be able to deliver the therapeutic genes efficiently and safely into patient cells. Many gene therapy protocols are currently being trialled clinically. These protocols, based mostly on the use of attenuated viruses to deliver the genes, carry potential risks to the patients in terms of infection, immune response, and germline modification. We have developed the first stage of a new technology for gene delivery that does not require the use of viruses. This technology is based on the generation of human artificial chromosomes, which are smaller versions of the naturally occurring chromosomes that carry all the genes inside our cells. Safety in these artificial chromosomes comes from the use of entirely human materials for their engineering. These artificial chromosomes also have other advantages over the viral approaches, including allowing large genes to be carried, and providing a permanent cure in a single treatment. We have already successfully constructed, published, and patented a number of first-generation human artificial chromosomes. The current project aims to complete the next proof-of-concept milestone towards the further development of this technology. Specifically, we propose to demonstrate the ability of the artificial chromosomes to carry genes and provide sustainable expression of these genes in cells and in animal models. Success in this study will allow the technology to proceed rapidly into commercialisation and clinical trial as a new improved tool for gene delivery and gene therapy.Read moreRead less
Methylation-sensitive T Cell Genes And Childhood Food Allergy.
Funder
National Health and Medical Research Council
Funding Amount
$461,232.00
Summary
Australia has the highest reported prevalence food allergy in the world. Despite this, little is known about how allergy develops. Mounting evidence implicates environmentally induced disruption of the genetic blueprint via a process known as epigenetics. We are combining the strengths of food challenge proven food allergy with assessment of immune functioning & cutting edge genomics, to extensively characterise the pathways leading to food allergy in children.
Methylation Sensitive Genes And The Transition To Allergic Disease: A Twin Study
Funder
National Health and Medical Research Council
Funding Amount
$493,843.00
Summary
Australia has amongst the highest reported prevalence allergic conditions (including asthma) in the world. Despite this, little is known about how these conditions arise. Mounting evidence implicates environmentally induced disruption of the genetic blueprint via a process known as epigenetics. We are combining the strengths of a unique collection of identical twins where one of a pair is sensitive to house dust mite, with cutting edge genomics, to characterise the pathways leading to allergy in ....Australia has amongst the highest reported prevalence allergic conditions (including asthma) in the world. Despite this, little is known about how these conditions arise. Mounting evidence implicates environmentally induced disruption of the genetic blueprint via a process known as epigenetics. We are combining the strengths of a unique collection of identical twins where one of a pair is sensitive to house dust mite, with cutting edge genomics, to characterise the pathways leading to allergy in children.Read moreRead less
Pathogen recognition and plant-defence activation by a novel Fusarium wilt-resistance protein from tomato. The devastating effects of Fusarium wilt disease of tomato is a threat to one of Australia's most economically important horticultural crops. Resistant tomato varieties offer the most effective means of control but the fundamental mechanisms underlying this resistance are yet to be understood. This research will increase our understanding of resistance to Fusarium wilt disease. The knowledg ....Pathogen recognition and plant-defence activation by a novel Fusarium wilt-resistance protein from tomato. The devastating effects of Fusarium wilt disease of tomato is a threat to one of Australia's most economically important horticultural crops. Resistant tomato varieties offer the most effective means of control but the fundamental mechanisms underlying this resistance are yet to be understood. This research will increase our understanding of resistance to Fusarium wilt disease. The knowledge gained will assist in the development of new robust, sustainable approaches to disease control, as well as the development of pre-emptive strategies to avert major outbreaks, which will ensure reliable productivity and minimal economic losses into the future.Read moreRead less
Translocation of secreted effector proteins from fungal pathogens into host plant cells. Every year, fungal diseases of plants cause huge losses in agricultural productivity and extensive environmental damage in Australia. Disease control in major crops, like wheat, currently relies heavily on breeding for disease resistance. However, fungal pathogens continually adapt to overcome plant defences, necessitating identification of new sources of resistance. The research in this project will eluc ....Translocation of secreted effector proteins from fungal pathogens into host plant cells. Every year, fungal diseases of plants cause huge losses in agricultural productivity and extensive environmental damage in Australia. Disease control in major crops, like wheat, currently relies heavily on breeding for disease resistance. However, fungal pathogens continually adapt to overcome plant defences, necessitating identification of new sources of resistance. The research in this project will elucidate the molecular basis of a new aspect of the establishment of plant infection by fungi, and in so doing will provide new avenues for the development of novel disease resistance strategies, with relevance in particular to devastating cereal diseases like wheat rust.Read moreRead less
Role of fungal secreted proteins as plant disease effectors. Many crop diseases are economically significant threats to agricultural productivity in Australia, with rust fungi in particular being a major problem for cereal grain production. Current methods of rust disease control are based on breeding for resistance but continued adaption by rust fungi to overcome plant defences means there is an urgent need for new methods of crop protection. This project will investigate molecular processes ....Role of fungal secreted proteins as plant disease effectors. Many crop diseases are economically significant threats to agricultural productivity in Australia, with rust fungi in particular being a major problem for cereal grain production. Current methods of rust disease control are based on breeding for resistance but continued adaption by rust fungi to overcome plant defences means there is an urgent need for new methods of crop protection. This project will investigate molecular processes underlying fungal infection of plants, focusing on mechanisms that enable fungi to take over the metabolism of infected cells. The research will provide basic knowledge for development of novel and durable disease resistance strategies.Read moreRead less
Early Nutrition, Epigenetics And Developmental Outcomes In Children.
Funder
National Health and Medical Research Council
Funding Amount
$550,093.00
Summary
Increasing evidence demonstrates that early nutrition, including in pregnancy, has long lasting programming effects on later health of children. Evidence implicates epigenetics (literally ‘above DNA’) in mediating these effects. The most convincing data exists for obesity and related disorders such as metabolic syndrome, diabetes, and heart disease. The focus of this project is on understanding how ‘epigenetic’ mechanisms are influencing the way our genes function to cause these disorders.
Plant immunity to fungal and bacterial pathogens. Since 2003, the Australian wheat crop has been threatened by a continuing stripe rust epidemic, which has required an additional production expense of at least $100 million per annum in fungicides. This Australian National University (ANU) - Commonwealth Scientific and Industrial Research Organisation (CSIRO) joint proposal aims to exploit the next-generation genome sequencing and associated bioinformatic and proteomic methods which are poised to ....Plant immunity to fungal and bacterial pathogens. Since 2003, the Australian wheat crop has been threatened by a continuing stripe rust epidemic, which has required an additional production expense of at least $100 million per annum in fungicides. This Australian National University (ANU) - Commonwealth Scientific and Industrial Research Organisation (CSIRO) joint proposal aims to exploit the next-generation genome sequencing and associated bioinformatic and proteomic methods which are poised to revolutionise biology to investigate the wheat-fungus interaction. We will develop new effective approaches for environmentally benign stripe rust control based on new knowledge about how this fungus causes disease and avoids the wheat's immune surveillance system.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0989105
Funder
Australian Research Council
Funding Amount
$495,000.00
Summary
An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry. Biomolecular research and research training, in which proteomics is core, has become a critical component of post-industrial development in the Hunter region. Development of a cutting edge proteomics facility will benefit a research community comprising over 50 researchers and 150 undergraduate students significantly enhancing their research productivity and translation of outcomes in areas of national i ....An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry. Biomolecular research and research training, in which proteomics is core, has become a critical component of post-industrial development in the Hunter region. Development of a cutting edge proteomics facility will benefit a research community comprising over 50 researchers and 150 undergraduate students significantly enhancing their research productivity and translation of outcomes in areas of national importance. These include understanding the impact of the environment on plant and animal development, pest animal control, development of new biotechnology tools, new drugs and new methods for the detection of narcotics and explosives.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0561173
Funder
Australian Research Council
Funding Amount
$207,189.00
Summary
High throughput proteomics - Thermo Finnigan ProteomeX LCQ Integrated Proteomics Workstation. As research in the biological sciences moves into post-genomics era, so attention has focused on the development of technologies capable of characterizing the molecular complexity inherent in the proteome. Recent technical innovations in this field have resulted in the advancement of mass spectrometers that are capable of exemplifying unknown proteins with great efficiency. These new technologies are ....High throughput proteomics - Thermo Finnigan ProteomeX LCQ Integrated Proteomics Workstation. As research in the biological sciences moves into post-genomics era, so attention has focused on the development of technologies capable of characterizing the molecular complexity inherent in the proteome. Recent technical innovations in this field have resulted in the advancement of mass spectrometers that are capable of exemplifying unknown proteins with great efficiency. These new technologies are central to any institution committed to the development of a competitive research nexus in biological sciences. The purpose of this application is to upgrade the mass spectrometry facility at the University of Newcastle such that it is able to provide cutting edge support to the extensive scientific community within the Hunter region.Read moreRead less