Identification of Biological pathways regulated by circular RNAs. Circular RNAs (circRNAs) are a, recently discovered molecule. circRNAs are highly abundant and expressed in a tissue and disease specific manner. Yet, currently the understanding of how circRNAs regulate biological processes is very poor. This project aims to use pooled shRNA libraries to screen a large panel of cell lines and systematically identify cellular activities that are regulated by circRNAs. The expected outcome of this ....Identification of Biological pathways regulated by circular RNAs. Circular RNAs (circRNAs) are a, recently discovered molecule. circRNAs are highly abundant and expressed in a tissue and disease specific manner. Yet, currently the understanding of how circRNAs regulate biological processes is very poor. This project aims to use pooled shRNA libraries to screen a large panel of cell lines and systematically identify cellular activities that are regulated by circRNAs. The expected outcome of this study will be a catalogue of functionally active circRNAs. Over the past decades, the wealth of knowledge on the function of linear mRNAs has had a significant impact on medicine and agriculture. Similarly understanding how circRNAs regulate cellular activities may have an analogous impact on humans.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE200100584
Funder
Australian Research Council
Funding Amount
$424,636.00
Summary
DNA Replication Stress: Characterizing ground zero for genomic instability. The overarching goal of this research is to gain insights into key underlying molecular causes of, and responses to, DNA replication stress - one of the most fundamental biological processes enabling life as we know it. I will use advanced interdisciplinary imaging techniques to directly visualize DNA replication stress structures within cells in order to better understand how cells stop this stress from progressing into ....DNA Replication Stress: Characterizing ground zero for genomic instability. The overarching goal of this research is to gain insights into key underlying molecular causes of, and responses to, DNA replication stress - one of the most fundamental biological processes enabling life as we know it. I will use advanced interdisciplinary imaging techniques to directly visualize DNA replication stress structures within cells in order to better understand how cells stop this stress from progressing into DNA damage. Insights into these processes will have the potential to improve preventative and therapeutic approaches to diseases such as cancer and neurodegeneration. Read moreRead less
Endocardial sprouting and mechano-signalling in heart trabeculation. This project aims to understand how the ventricles, the pumping chambers of the mammalian heart, form during embryonic life. Critical is the elaboration of trabeculae, myocardial projections that form a sponge-like layer on the inner surface of the chamber wall and which play vital roles in contraction, oxygen and nutrient exchange, conduction and septation. The project expects to develop a deeper understanding of trabeculation ....Endocardial sprouting and mechano-signalling in heart trabeculation. This project aims to understand how the ventricles, the pumping chambers of the mammalian heart, form during embryonic life. Critical is the elaboration of trabeculae, myocardial projections that form a sponge-like layer on the inner surface of the chamber wall and which play vital roles in contraction, oxygen and nutrient exchange, conduction and septation. The project expects to develop a deeper understanding of trabeculation using high resolution, single cell methodologies, and to investigate how bio-mechanical forces from contraction or blood flow influence chambers formation.Read moreRead less