Improving plant productivity and human health using next generation biotechnology approaches. Both medical and plant sciences face similar technological problems in harnessing the power of modern DNA sequencing for accelerating the pace of beneficial gene function discovery. Plant and animal researchers will collaborate in this program to meet this common challenge. The research outcomes envisaged in this proposal will benefit human health by enabling more rapid discovery of genes related to obe ....Improving plant productivity and human health using next generation biotechnology approaches. Both medical and plant sciences face similar technological problems in harnessing the power of modern DNA sequencing for accelerating the pace of beneficial gene function discovery. Plant and animal researchers will collaborate in this program to meet this common challenge. The research outcomes envisaged in this proposal will benefit human health by enabling more rapid discovery of genes related to obesity, immunity, fertility, neurological function and cancer. In the plant sphere, the outcomes will shed new light on plant functions related to plant energy metabolism, vitamin biosynthesis drought tolerance and water use by crops. The research will benefit both human health and agricultural food production and quality.Read moreRead less
Biology of flowering plant male gametic cells in relation to fertilization. The world food supply is primarily dependent on plants. The continuation of an adequate food supply depends upon the success of fertilization that involves the fusion of the sperm cell with the egg cell. The entire process of fertilization can be manipulated once the specific molecules involved in its regulation have been identified. This project deals with the discovery of male gamete biomolecules involved in fertili ....Biology of flowering plant male gametic cells in relation to fertilization. The world food supply is primarily dependent on plants. The continuation of an adequate food supply depends upon the success of fertilization that involves the fusion of the sperm cell with the egg cell. The entire process of fertilization can be manipulated once the specific molecules involved in its regulation have been identified. This project deals with the discovery of male gamete biomolecules involved in fertilization. The knowledge obtained will have potential applications in developing novel breeding technologies for improved crop plants.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0453608
Funder
Australian Research Council
Funding Amount
$579,230.00
Summary
Multifunctional confocal laser scanning microscope with time resolved and two photon imaging and fluorescence correlation capabilities. We seek to establish an Australian first confocal laser scanning microscope with time resolved imaging and fluorescence correlation spectroscopy capabilities. Its advantages include ultrasensitive detection of weak fluorescent emissions against high autofluorescent background by using fluorescence lifetime signatures, with over three orders of magnitude discrimi ....Multifunctional confocal laser scanning microscope with time resolved and two photon imaging and fluorescence correlation capabilities. We seek to establish an Australian first confocal laser scanning microscope with time resolved imaging and fluorescence correlation spectroscopy capabilities. Its advantages include ultrasensitive detection of weak fluorescent emissions against high autofluorescent background by using fluorescence lifetime signatures, with over three orders of magnitude discrimination improvement. The system will also be able to monitor binding of single molecules. These techniques will open new and exciting avenues for interdisciplinary research at the frontier between biological and physical sciences. The microscope will operate within an existing multi-user Optical Characterisation Facility supporting research of an established network of scientists in the Sydney area.Read moreRead less
How plants produce their biomass. This project aims to investigate mechanisms that underpin the formation of secondary walls, the bulk of biomass in plant cells. Plant cell walls are essential for plant growth and provide great raw materials for many industrial products. Understanding how cell walls are made would enable tailored plant biomass production, but understanding remains poor. The project will induce secondary walls at will and outline a framework for how secondary walls are made. The ....How plants produce their biomass. This project aims to investigate mechanisms that underpin the formation of secondary walls, the bulk of biomass in plant cells. Plant cell walls are essential for plant growth and provide great raw materials for many industrial products. Understanding how cell walls are made would enable tailored plant biomass production, but understanding remains poor. The project will induce secondary walls at will and outline a framework for how secondary walls are made. The outcomes are expected to be relevant for the fuel, feed, food and construction sectors, and thus to Australia's future.Read moreRead less
Why are many fungicide lead compounds active against pathogens in axenic culture but inactive when applied to infected plants? It is much cheaper and easier to screen compounds for fungicidal activity in vivo (that is against the fungus grown in axenic culture) than to test in planta. However, it is commonly observed that compounds active in in vivo screens do not subsequently prove to be active in planta. The aim of this project is to investigate the physiological, biochemical and genetics basi ....Why are many fungicide lead compounds active against pathogens in axenic culture but inactive when applied to infected plants? It is much cheaper and easier to screen compounds for fungicidal activity in vivo (that is against the fungus grown in axenic culture) than to test in planta. However, it is commonly observed that compounds active in in vivo screens do not subsequently prove to be active in planta. The aim of this project is to investigate the physiological, biochemical and genetics basis of this discrepancy and to attempt to provide tools that circumvent the problem. It is expected the project will generate novel information on the environment in the plant experienced by the fungus.Read moreRead less
Targeted analysis of the arbuscular mycorrhizal symbiosis phenome in a model host, tomato. We will capitalise on our previous discovery of novel phenotypic variation in arbuscular mycorrhizas in mutant and wild-type tomato, to explore development and function of the symbiosis at the molecular-genetic level. We will clone and sequence the gene responsible for mycorrhiza-defective phenotypes to provide inferences on function and relations with other genes. We will determine if plant defence blocks ....Targeted analysis of the arbuscular mycorrhizal symbiosis phenome in a model host, tomato. We will capitalise on our previous discovery of novel phenotypic variation in arbuscular mycorrhizas in mutant and wild-type tomato, to explore development and function of the symbiosis at the molecular-genetic level. We will clone and sequence the gene responsible for mycorrhiza-defective phenotypes to provide inferences on function and relations with other genes. We will determine if plant defence blocks fungal colonisation in the mutant and/or varies with different wild-type phenotypes and explore molecular mechanisms of nutrient transfer from fungus to plant in relation to phenotypic diversity. The project will provide new insights into genome/phenome interactions controlling this widespread beneficial symbiosis.Read moreRead less
Australian rushes: unearthing the function of root clusters and sand-binding roots. Unearthing the functioning of highly specialised root structures provides fundamental insights into the role of native rush plants in south-western Australian ecosystems and addresses a major issue with Australia's biodiversity, currently a Priority area for the ARC. Native rushes form a prominent but inconspicuous component of the Australian 'grass-like' flora, accounting for more than half the plant biomass on ....Australian rushes: unearthing the function of root clusters and sand-binding roots. Unearthing the functioning of highly specialised root structures provides fundamental insights into the role of native rush plants in south-western Australian ecosystems and addresses a major issue with Australia's biodiversity, currently a Priority area for the ARC. Native rushes form a prominent but inconspicuous component of the Australian 'grass-like' flora, accounting for more than half the plant biomass on some landscapes. Rushes are also highly sensitive to small increments in nutrients in disturbed environments and thus form a management priority relating to their use in rehabilitation of degraded landscapes, such as mine sites and wetland margins. Read moreRead less
The impact of water logging on mitochondrial metabolism in leaves and roots of Grey poplar. Water logging and flooding are seasonal occurrences in a variety of environments. These events cause various damage to environments, including damage to plants. The damage to plants has traditionally been associated with annuals, such as various crop species. However larger plants, i.e. trees, are also very susceptible to water-logging and flooding. As it can take from several years to decades for tress t ....The impact of water logging on mitochondrial metabolism in leaves and roots of Grey poplar. Water logging and flooding are seasonal occurrences in a variety of environments. These events cause various damage to environments, including damage to plants. The damage to plants has traditionally been associated with annuals, such as various crop species. However larger plants, i.e. trees, are also very susceptible to water-logging and flooding. As it can take from several years to decades for tress to be established it is important to understand how trees can withstand water-logging and flooding. As tree plantation are uses for commercial and environmental purposes this knowledge will be useful to a broad spectrum of users in the community.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE120101562
Funder
Australian Research Council
Funding Amount
$375,000.00
Summary
Quantifying the contribution of leaf vein networks to the leaf economics spectrum in native and agricultural species. Using a combination of eco-physiological and geometric measures this project will evaluate the influence of leaf vein networks on leaf economics. It is expected that this work will identify vein investment and network design as major sources of variability underlying species adaptive strategies, and the global leaf economics spectrum as a whole.
New approaches to unravelling post-translational controls operating on the cyanobacterial carbon dioxide concentrating mechanism. Marine blue-green algae contribute to global primary productivity but their carbon dioxide acquisition processes are poorly understood. The project will employ mutagenesis and genome sequencing to discover the controls that regulate carbon dioxide uptake processes required for efficient photosynthesis and use this data to aid in engineering crop plants that use less w ....New approaches to unravelling post-translational controls operating on the cyanobacterial carbon dioxide concentrating mechanism. Marine blue-green algae contribute to global primary productivity but their carbon dioxide acquisition processes are poorly understood. The project will employ mutagenesis and genome sequencing to discover the controls that regulate carbon dioxide uptake processes required for efficient photosynthesis and use this data to aid in engineering crop plants that use less water.Read moreRead less