Subunit Contacts in a Replicative DNA Polymerase: A New Paradigm for Protein-Protein Interactions? The bacterial DNA polymerase III is a 15-subunit protein that acts as an extraordinary molecular machine to copy both strands of chromosomal DNA at the same time, making DNA at the rate of 1000 base pairs each second without ever falling off the chromosome or making mistakes. This project aims to understand the way its subunits iteract, such that they can form stable complexes that are nevertheless ....Subunit Contacts in a Replicative DNA Polymerase: A New Paradigm for Protein-Protein Interactions? The bacterial DNA polymerase III is a 15-subunit protein that acts as an extraordinary molecular machine to copy both strands of chromosomal DNA at the same time, making DNA at the rate of 1000 base pairs each second without ever falling off the chromosome or making mistakes. This project aims to understand the way its subunits iteract, such that they can form stable complexes that are nevertheless flexible enough to accomplish DNA synthesis. There are applications of this knowledge to discovery of new antibacterial agents and in design of new protein machines.Read moreRead less
Quantifying the role of the Southern Ocean for anthropogenic CO2 uptake. Carbon dioxide (CO2) is the most important greenhouse gas contributing to global warming and climate change. Climate change is likely to have dramatic economic and environmental consequences for Australia. Knowledge and understanding of the complex carbon cycle is fundamental for predicting future atmospheric CO2 levels and managing climate change. The aim of the work proposed here is to quantify and improve our understan ....Quantifying the role of the Southern Ocean for anthropogenic CO2 uptake. Carbon dioxide (CO2) is the most important greenhouse gas contributing to global warming and climate change. Climate change is likely to have dramatic economic and environmental consequences for Australia. Knowledge and understanding of the complex carbon cycle is fundamental for predicting future atmospheric CO2 levels and managing climate change. The aim of the work proposed here is to quantify and improve our understanding of the oceans role in controlling atmospheric CO2 levels. This will be done by combining modeling and observational expertise among UNSW and CSIRO (Marine Research) researchers. Our work will be the first to assess the extent of which the Southern Ocean (and Australian waters) acts as a carbon sink. This will reduce modeling uncertainties in predicting future atmospheric CO2 levels and will also be valuable to the federal government in future international negotiations on climate change.Read moreRead less
Atomisation and Combustion Physics of Australian Bio-oils. Australia is highly dependent on fossil fuels for energy production and transport, and this dependence is growing. Wide spread substitution of liquid hydrocarbon fuels by indigenous renewable bio-oil has the potential to improve Australian's energy outlook and assist in reaching greenhouse gas targets. Understanding the interrelationships between the physical and chemical properties of bio-oil, its atomisation, droplet formation and com ....Atomisation and Combustion Physics of Australian Bio-oils. Australia is highly dependent on fossil fuels for energy production and transport, and this dependence is growing. Wide spread substitution of liquid hydrocarbon fuels by indigenous renewable bio-oil has the potential to improve Australian's energy outlook and assist in reaching greenhouse gas targets. Understanding the interrelationships between the physical and chemical properties of bio-oil, its atomisation, droplet formation and combustion physics is fundamental to the delivery of an efficient and reliable combustion process using this fuel. Measurements using laser based diagnostics of the atomisation flow, droplet formation and combustion process will provide the experimental data to understand this complex interrelationship.Read moreRead less
The Cytochrome P450 Gene Super-family in Drosophila melanogaster; Gene Function and Insecticide Resistance. The cytochrome P450 (Cyp) gene super-family is represented by over 90 sequences in the genome of the vinegar fly, Drosophila melanogaster. To date, four Cyp genes are found to be involved in insecticide resistance. The function of the majority of Cyp genes is unknown. This project will investigate the function and regulation of D. melanogaster Cyp genes, linking the fly's genotype to its ....The Cytochrome P450 Gene Super-family in Drosophila melanogaster; Gene Function and Insecticide Resistance. The cytochrome P450 (Cyp) gene super-family is represented by over 90 sequences in the genome of the vinegar fly, Drosophila melanogaster. To date, four Cyp genes are found to be involved in insecticide resistance. The function of the majority of Cyp genes is unknown. This project will investigate the function and regulation of D. melanogaster Cyp genes, linking the fly's genotype to its phenotype. By studying the effects of Cyp genes on fly survival, Cyp gene expression and regulation, and expressing selected Cyp genes in a yeast expression system, we will enhance our understanding of Cyp gene function and evolution.Read moreRead less