Linkage Infrastructure, Equipment And Facilities - Grant ID: LE100100078
Funder
Australian Research Council
Funding Amount
$600,000.00
Summary
Multiphoton confocal microscope. Recent developments in light microscopy have revolutionised modern molecular and cellular biology. Dramatic improvements in microscope hardware and software and in the range of fluorescent markers used to tag selected cellular components now provide new and exciting opportunities to localise and determine the function of ions and molecules not only in preserved samples but also, most excitingly, in living cells. The proposed multiphoton confocal microscope will ....Multiphoton confocal microscope. Recent developments in light microscopy have revolutionised modern molecular and cellular biology. Dramatic improvements in microscope hardware and software and in the range of fluorescent markers used to tag selected cellular components now provide new and exciting opportunities to localise and determine the function of ions and molecules not only in preserved samples but also, most excitingly, in living cells. The proposed multiphoton confocal microscope will allow researchers in Canberra to obtain high quality images of static and moving components in living cells and tissues and will facilitate the discovery of new knowledge that contributes to our understanding and control of development and disease in both plants and animals.Read moreRead less
Proteome mapping of the model fungal plant pathogen Stagonospora nodorum using LC-LC-MS/MS. Stagonospora nodorum is a fungus that causes leaf and glume blotch disease on wheat. This disease alone causes $55 million dollars in yield losses per annum in Australia. This project aims to identify the proteins produced by Stagonospora nodorum through the development of a new proteomics technique. Two clear benefits to the community resulting from this project will emerge. The first will be the expert ....Proteome mapping of the model fungal plant pathogen Stagonospora nodorum using LC-LC-MS/MS. Stagonospora nodorum is a fungus that causes leaf and glume blotch disease on wheat. This disease alone causes $55 million dollars in yield losses per annum in Australia. This project aims to identify the proteins produced by Stagonospora nodorum through the development of a new proteomics technique. Two clear benefits to the community resulting from this project will emerge. The first will be the expert training of a student in proteomics, a skill that is keenly sought. Secondly, the identification of these pathogen proteins will lead to new strategies to better control the disease and secure the supply of wheat.Read moreRead less
Functional characterisation of the necrotrophic effector proteins Tox1 and Tox3 from the wheat pathogen Stagonospora nodorum. Fungal pathogens cost the Australian agricultural industry over one billion dollars per year. This project will build upon recent key advances to provide a fundamental basis on how fungal pathogens cause disease. The results from this study will promote future advances in disease management with the aim of securing Australian wheat supplies.
Isolation and functional characterisation of a pathogen meta effector able to inhibit detection of multiple disease effectors by resistant plants. The rust fungi are a major economic threat to crop production in Australia. This project will investigate the molecular mechanism used by a rust fungus to prevent detection of multiple disease-inducing proteins by resistant plants and generate knowledge that will lead to the development of new and more effective disease control strategies.
The identification of Mycosphaerella graminicola effectors that promote pathogenicity on wheat. Fungal diseases are one of the greatest challenges to sustainable wheat production in the 21st century. Septoria tritici blotch is one such disease as it inflicts millions of tonnes in yield losses per annum. This project will identify the molecular basis of Septoria tritici blotch and assess its potential as an Australian biosecurity threat.
Control of sucrose source/sink status by the plant immune system. This project aims to investigate the roles of plant malectin receptor kinases in pathogen recognition and response. The project will utilise molecular and cell biological tools to detect complex formation and changes in sugar import into cells. Expected outcomes of this project include a better understanding of how pathogens manipulate plant cells to their benefit, and greater knowledge of which host molecules participate in this ....Control of sucrose source/sink status by the plant immune system. This project aims to investigate the roles of plant malectin receptor kinases in pathogen recognition and response. The project will utilise molecular and cell biological tools to detect complex formation and changes in sugar import into cells. Expected outcomes of this project include a better understanding of how pathogens manipulate plant cells to their benefit, and greater knowledge of which host molecules participate in this process. This should provide significant benefits such as a new theoretical basis to engineer crop plants for resistance against devastating diseases.Read moreRead less
Identification of immune receptor and signalling proteins from plants. This project aims to clone a new extracellular pathogen receptor, and map immune signalling pathways downstream of both intra- and extra-cellular receptors using innovative biochemical methods. The plant immune system protects plants and crops from attack by pests and pathogens. It is an innate system based on extracellular and intracellular pathogen receptors. Despite the importance of plant immunity in both biological and a ....Identification of immune receptor and signalling proteins from plants. This project aims to clone a new extracellular pathogen receptor, and map immune signalling pathways downstream of both intra- and extra-cellular receptors using innovative biochemical methods. The plant immune system protects plants and crops from attack by pests and pathogens. It is an innate system based on extracellular and intracellular pathogen receptors. Despite the importance of plant immunity in both biological and agricultural terms, little is known about the identity of such receptors or the signalling events that link pathogen perception to response. The results are expected to enhance crop productivity and provide important insights into the architecture of the plant immune system.Read moreRead less
Molecular characterisation of the Prf bacterial recognition complex of tomato. This project will investigate the composition and function of a large protein complex in tomatoes that controls their ability to resist attack by bacteria. The results will provide understanding to how all plants resist all diseases, and may help to improve environmentally-benign disease resistance in crop species.
Discovery Early Career Researcher Award - Grant ID: DE130101292
Funder
Australian Research Council
Funding Amount
$375,000.00
Summary
Plant immune receptors: what are the first steps that trigger defence signalling? Plant immune receptors that confer resistance to infectious disease will be investigated at a molecular level. The outcomes of this study will influence the development of new strategies to protect Australian crops from destructive disease and reduce the use of pesticides.
Molecular basis of rust infection and host plant resistance. Plant diseases threaten agricultural productivity in Australia, with rust fungi being a major problem for cereal grain production. This project will investigate molecular processes underlying the infection of plants by rust fungi and will provide basic knowledge for development of novel and durable disease resistance strategies.