Linkage Infrastructure, Equipment And Facilities - Grant ID: LE180100001
Funder
Australian Research Council
Funding Amount
$345,475.00
Summary
Pushing the limits of fluorescence microscopy with adaptive optics. This project aims to establish an adaptive optics, super-resolution optical microscopy facility to image cellular events with the highest possible spatial resolution, in a whole cell or tissue context. Sophisticated computer-controlled deformable mirrors will be used to correct the way light is distorted as it passes through specimens, thereby overcoming aberrations found in thick and complex samples. This adaptive optics system ....Pushing the limits of fluorescence microscopy with adaptive optics. This project aims to establish an adaptive optics, super-resolution optical microscopy facility to image cellular events with the highest possible spatial resolution, in a whole cell or tissue context. Sophisticated computer-controlled deformable mirrors will be used to correct the way light is distorted as it passes through specimens, thereby overcoming aberrations found in thick and complex samples. This adaptive optics system will enable researchers to study complex behaviour of biological specimens, at the optical resolution limit in plant and animal tissues, leading to basic biology and biotechnology outcomes in biofuels, biomaterials and biomedicines.Read moreRead less
Differential roles of gene family members in development of a cell lineage. This project aims to investigate how a family of genes influence cells in the testis to become mature sperm. Testicular cells regulate gene activity via the Snail family of proteins during sperm development, and interruption of their activities reduces fertility in mice and fruit flies. The project aims to use genetic, cell biological and biochemical studies in Drosophila and mice to compare different Snail family protei ....Differential roles of gene family members in development of a cell lineage. This project aims to investigate how a family of genes influence cells in the testis to become mature sperm. Testicular cells regulate gene activity via the Snail family of proteins during sperm development, and interruption of their activities reduces fertility in mice and fruit flies. The project aims to use genetic, cell biological and biochemical studies in Drosophila and mice to compare different Snail family proteins in spermatogenesis. The outcomes will define the different roles of highly similar proteins from the same family in differentiation of a single cell lineage. This is important in generating functional tissues using in vitro laboratory approaches or understanding how normal development and developmental disorders arise.Read moreRead less
Understanding the potency and role of individual stem cells in the skin using Rainbow technology. To renew itself, the skin and its components rely on the activity of stem cells. This project will define more precisely the role of each individual stem cell by labelling them with a unique colour and following its fate. This project has the potential to change our current view on how the skin maintains and repairs itself.
Understanding the diverse biology of CD4+ T cell resident memory. This project aims to examine the biology of CD4 T cell memory in tissues. The previously unappreciated complexity of the CD4 T cell resident memory compartment in the liver will be characterised, focusing on the generation, maintenance and diversity of functions of these cells. Expected outcomes include the generation of fundamental knowledge in the disciplines of cellular biology and immunology, and unique, highly specialised stu ....Understanding the diverse biology of CD4+ T cell resident memory. This project aims to examine the biology of CD4 T cell memory in tissues. The previously unappreciated complexity of the CD4 T cell resident memory compartment in the liver will be characterised, focusing on the generation, maintenance and diversity of functions of these cells. Expected outcomes include the generation of fundamental knowledge in the disciplines of cellular biology and immunology, and unique, highly specialised student and personnel training through the interdisciplinary approach utilised, which spans cellular biology, live-imaging and transcriptomic analyses. Expected benefits include influential publications and the import of a novel, specialised technique to Australia through an international collaboration (Germany)Read moreRead less
What drives the Anterior Expansion of the Central Nervous System? A striking and highly conserved feature of the central nervous system is that the brain is larger than the spinal cord. Despite the manifest implications this has for nervous system function, the underlying drivers are largely unknown. This project aims to investigate the mechanisms controlling anterior expansion of the central nervous system, and will generate new knowledge in the areas of nervous system development and evolution ....What drives the Anterior Expansion of the Central Nervous System? A striking and highly conserved feature of the central nervous system is that the brain is larger than the spinal cord. Despite the manifest implications this has for nervous system function, the underlying drivers are largely unknown. This project aims to investigate the mechanisms controlling anterior expansion of the central nervous system, and will generate new knowledge in the areas of nervous system development and evolution. This project aims to impact on our understanding of nervous system function, develop bioinformatics tools with broad utility within the biosciences field, strengthen Australia’s international standing in the developmental neuroscience, and enhance the capacity for interdisciplinary international collaborations.Read moreRead less
Targeting mitochondria with mitocans to treat cancer: mechanistic aspects. Mitochondria are the power-house of the cell and also the reservoir of proteins causing the demise of cancer cells, therefore suppressing tumour progression. This project proposes a novel way to modify certain compounds, increasing their level in mitochondria in order to maximise their anti-cancer effect.
Mechanism and function of cell asymmetry during cell death. This project aims to investigate how dying cells rearrange their cellular contents to aid their removal.
More than 200 billions cells die daily in the human body. It is critical that dying cells are rapidly cleared as their buildup can interfere with normal tissue functions. This project will use a suite of contemporary molecular/cell biological approaches to study a newly identified process that occurs during cell death. Expected outc ....Mechanism and function of cell asymmetry during cell death. This project aims to investigate how dying cells rearrange their cellular contents to aid their removal.
More than 200 billions cells die daily in the human body. It is critical that dying cells are rapidly cleared as their buildup can interfere with normal tissue functions. This project will use a suite of contemporary molecular/cell biological approaches to study a newly identified process that occurs during cell death. Expected outcomes include a paradigm-shift in understanding the process of cell clearance.
This project is expected to generate fundamental new knowledge of the mechanisms by which dying cells are efficiently removed from tissues. This should provide significant benefits to the cell death and general cell biology fields.Read moreRead less
The lipidomics of cell fate. This project aims to dissect the roles of lipids in cell fate. The study of lipids, or lipidomics, is an emerging and exciting area of biological science. The fundamental roles of lipids in development remain vastly understudied. This project will look at reprogramming of somatic cells into stem cells, their pluripotency and differentiation. This will be complemented with studies in the zebrafish, which permits the direct study of cell fate in vivo. This approach is ....The lipidomics of cell fate. This project aims to dissect the roles of lipids in cell fate. The study of lipids, or lipidomics, is an emerging and exciting area of biological science. The fundamental roles of lipids in development remain vastly understudied. This project will look at reprogramming of somatic cells into stem cells, their pluripotency and differentiation. This will be complemented with studies in the zebrafish, which permits the direct study of cell fate in vivo. This approach is a powerful way to unlock major events involved in development and to unmask the roles of lipids in these fundamental mechanisms.Read moreRead less
Mechanism and function of dying cell disassembly. This project aims to elucidate the molecular machinery that disassembles dying cells, and the role of this process in cell clearance. Billions of cells in the body die daily as part of normal turnover. Dying cells must be rapidly removed, as their accumulation can interfere with normal tissue functions. To efficiently clear dead cells, dying cells can disassemble into smaller fragments that neighbouring cells engulf. Understanding the mechanistic ....Mechanism and function of dying cell disassembly. This project aims to elucidate the molecular machinery that disassembles dying cells, and the role of this process in cell clearance. Billions of cells in the body die daily as part of normal turnover. Dying cells must be rapidly removed, as their accumulation can interfere with normal tissue functions. To efficiently clear dead cells, dying cells can disassemble into smaller fragments that neighbouring cells engulf. Understanding the mechanistic basis and function of dying cell disassembly is expected to generate knowledge of the downstream consequence of cell death. This breakthrough will be important in many fields of research including cell biology and biochemistry, and generate basic knowledge that can ultimately be applied in medical science to understand or treat pathological conditions associated with cell death.Read moreRead less