Linkage Infrastructure, Equipment And Facilities - Grant ID: LE140100111
Funder
Australian Research Council
Funding Amount
$475,000.00
Summary
Expanding the Genomic Frontier - from Species to Strains and Individuals to Populations. Expanding the genomic frontier from species to strains and individuals to populations: The Ramaciotti Centre for Gene Function Analysis, a consortium of five universities, provides a large number of genomics and transcriptomics analyses. This project will establish an Ion Proton semiconductor-based sequencer and iScan platform to facilitate research breakthroughs in genomics, epigenomics, transcriptomics, an ....Expanding the Genomic Frontier - from Species to Strains and Individuals to Populations. Expanding the genomic frontier from species to strains and individuals to populations: The Ramaciotti Centre for Gene Function Analysis, a consortium of five universities, provides a large number of genomics and transcriptomics analyses. This project will establish an Ion Proton semiconductor-based sequencer and iScan platform to facilitate research breakthroughs in genomics, epigenomics, transcriptomics, and SNP analysis. Cell screening technology will also be established to allow the rapid analysis of cells of interest, prior to genomic / transcriptomic analysis. The increased data output, and concomitant reduction in analysis cost on the new platforms, will expand the genomics frontier, allowing researchers to fully analyse many strains from a single-celled species or many individuals from a population.Read moreRead less
Uncovering microRNA decay regulation in mammalian cells. MicroRNAs (miRNAs) constitute a novel mechanism used by cells to regulate gene expression, however, very little is known about the mechanisms affecting miRNA accumulation. Characterisation of the kinetics of miRNA turnover is of paramount importance to establish the reliability of miRNAs as novel biomarkers. This project aims to characterise miRNA stability in mammalian cells, investigate mechanisms of turnover and establish their importan ....Uncovering microRNA decay regulation in mammalian cells. MicroRNAs (miRNAs) constitute a novel mechanism used by cells to regulate gene expression, however, very little is known about the mechanisms affecting miRNA accumulation. Characterisation of the kinetics of miRNA turnover is of paramount importance to establish the reliability of miRNAs as novel biomarkers. This project aims to characterise miRNA stability in mammalian cells, investigate mechanisms of turnover and establish their importance on the regulatory function of miRNAs. Such information is critical in the future development of targeted therapeutics.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE120100091
Funder
Australian Research Council
Funding Amount
$250,000.00
Summary
A five laser multichannel flow cytometry cell sorter for the University of New South Wales as part of an advanced flow cytometry network. Flow cytometry is a technique for counting and examining microscopic particles, such as cells and chromosomes, by suspending them in a stream of fluid and passing them by an electronic detection apparatus. This project will establish such advanced cell sorting instrumentation at the University of New South Wales, providing this capability to a wide range of re ....A five laser multichannel flow cytometry cell sorter for the University of New South Wales as part of an advanced flow cytometry network. Flow cytometry is a technique for counting and examining microscopic particles, such as cells and chromosomes, by suspending them in a stream of fluid and passing them by an electronic detection apparatus. This project will establish such advanced cell sorting instrumentation at the University of New South Wales, providing this capability to a wide range of researchers in diverse fields. The project will also provide a basis for establishing a flow cytometry network with partner institutes University of Sydney and the University of Technology, Sydney.Read moreRead less
Discovery of bioactive natural substances from uncultured bacteria and their production using photosynthetic reactor technology. The range and rate of natural product discovery is the limiting factor in developing new therapies for cancer and infectious disease. This research will enable the discovery of new drugs, coupled to their production in a photosynthetic expression system. This represents a truly “green” and sustainable technology for the pharmaceutical industry.
Biology and evolution of intracellular parasitism. This project will investigate the development of intracellular parasitism in environmental amoebae. The outcomes of this work will help to understand the mechanisms by which bacteria have evolved to survive inside cells and in some cases cause disease.
Discovery Early Career Researcher Award - Grant ID: DE140101728
Funder
Australian Research Council
Funding Amount
$395,220.00
Summary
The regulation and evolution of posttranscriptional gene networks. The ability of cells to regulate gene expression is key for organism development, adaptation to new environments and evolutionary changes that shape the diversity of life on Earth. This project studies the RNA binding proteins called PUFs which are central for gene expression in diverse organisms. Using cutting-edge new generation systems biology approaches, this project will study how PUF proteins regulate genes to enable metabo ....The regulation and evolution of posttranscriptional gene networks. The ability of cells to regulate gene expression is key for organism development, adaptation to new environments and evolutionary changes that shape the diversity of life on Earth. This project studies the RNA binding proteins called PUFs which are central for gene expression in diverse organisms. Using cutting-edge new generation systems biology approaches, this project will study how PUF proteins regulate genes to enable metabolic adaptation, differentiation of cell types and the evolution of new gene expression outputs in distinct biological species. The outcomes will include new insights into the regulation and evolution of posttranscriptional gene networks. Read moreRead less
Epigenetic regulation in bacteria. This project aims to understand the effect of DNA modification on gene regulation in the bacterial organism Escherichia coli, which causes urinary tract infection worldwide. High-throughput DNA sequencing technologies mean one can determine the entire genetic blueprint of a bacterium – its genome – accurately, quickly and cheaply. Single-molecule real-time sequencing provides a complete read-out of a bacterial genome (genetic data) and chemical modifications of ....Epigenetic regulation in bacteria. This project aims to understand the effect of DNA modification on gene regulation in the bacterial organism Escherichia coli, which causes urinary tract infection worldwide. High-throughput DNA sequencing technologies mean one can determine the entire genetic blueprint of a bacterium – its genome – accurately, quickly and cheaply. Single-molecule real-time sequencing provides a complete read-out of a bacterial genome (genetic data) and chemical modifications of the DNA (epigenetic data). Epigenetic data can affect regulation: how genes are switched off and on. This project seeks to harness the power of single-molecule DNA sequencing, together with state-of-the-art genomic and molecular approaches, to better understand the impact of DNA modification on gene regulation in the model bacterial organism, Escherichia coli. This work will support advanced training in bioinformatics and microbiology and improve our understanding of regulation in all bacteria.Read moreRead less
The protein O-glycosylation pathway in Neisseria meningitidis. Neisseria meningitidis causes bacterial meningitis, a sudden and severe disease of particular concern to children in both the developed and developing worlds. This project will contribute to an understanding of how these bacteria evade the immune system by modifying the proteins displayed on their surface, which will help in the development of a vaccine.
Peril and promise: Origins and spread of integron gene cassettes. Integrons have a major role in spreading antibiotic resistance genes among pathogens. They do so by capturing gene cassettes encoding resistance, yet how these cassettes are generated, the taxa in which they originate, and the range of traits that cassettes can encode have been outstanding questions for 30 years. This project addresses these long standing questions. The project will analyze single bacterial cells to detect newly ....Peril and promise: Origins and spread of integron gene cassettes. Integrons have a major role in spreading antibiotic resistance genes among pathogens. They do so by capturing gene cassettes encoding resistance, yet how these cassettes are generated, the taxa in which they originate, and the range of traits that cassettes can encode have been outstanding questions for 30 years. This project addresses these long standing questions. The project will analyze single bacterial cells to detect newly generated cassettes and assign them to specific taxa, using an innovative method that links cassette DNA to bacterial 16S rDNA. Understanding cassette origins is the key to controlling their activity, both to harness integrons for biotechnology, and to prevent pathogens from acquiring new, dangerous traits. Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE140100166
Funder
Australian Research Council
Funding Amount
$370,000.00
Summary
Imaging Cell and Tissue Architecture using Confocal and Super-Resolution Microscopy. Imaging cell and tissue architecture using confocal and super-resolution microscopy: This project aims to understand how the architecture of cells and tissues is controlled. This is because the organisation of biological space underpins the function of cells, tissues and organisms. This project will test the role of identified parts of cell architecture in regulating specific animal functions/pathologies. It wil ....Imaging Cell and Tissue Architecture using Confocal and Super-Resolution Microscopy. Imaging cell and tissue architecture using confocal and super-resolution microscopy: This project aims to understand how the architecture of cells and tissues is controlled. This is because the organisation of biological space underpins the function of cells, tissues and organisms. This project will test the role of identified parts of cell architecture in regulating specific animal functions/pathologies. It will do this by using new microscope technologies which are at the frontier of visualising cell structure in isolation and in the context of tissue including application to the living animal. The dynamic organisation of structures in cells will be imaged in living tissue. Novel insights into structure/function relationships in the body will impact the health industry and generate opportunities for new diagnostics and therapeutics. Read moreRead less