Development of a novel high yield cell-free protein expression system. Recombinant proteins are used as vaccines, drugs, and research tools, as well as food and detergent additives, comprising a A$100 billion international market. Their production requires laborious, expensive, and time-consuming construction of transgenic organisms or cells. Alternatively, recombinant proteins can be produced in extracts prepared from cells or organisms. The aim of this proposal is to develop a new technology t ....Development of a novel high yield cell-free protein expression system. Recombinant proteins are used as vaccines, drugs, and research tools, as well as food and detergent additives, comprising a A$100 billion international market. Their production requires laborious, expensive, and time-consuming construction of transgenic organisms or cells. Alternatively, recombinant proteins can be produced in extracts prepared from cells or organisms. The aim of this proposal is to develop a new technology that will make cell-free production of recombinant proteins rapid, cheap, and scalable. This will advance Australia’s intellectual leadership in the area of biotechnology and will bring numerous economic benefits by accelerating pharmaceutical development. Read moreRead less
Development of novel fluorescent proteins from marine organisms for in vivo fluorescence imaging technologies and cancer research. The Australian Great Barrier Reef (GBR) is a national treasure and the development of fluorescent proteins (FPs) from reef organisms for in vivo imaging and biotechnology will enhance the appreciation of this resource. Nowadays, imaging of biochemical processes in living cells is essential for knowing the roles of various genes and proteins in health and diseases. Th ....Development of novel fluorescent proteins from marine organisms for in vivo fluorescence imaging technologies and cancer research. The Australian Great Barrier Reef (GBR) is a national treasure and the development of fluorescent proteins (FPs) from reef organisms for in vivo imaging and biotechnology will enhance the appreciation of this resource. Nowadays, imaging of biochemical processes in living cells is essential for knowing the roles of various genes and proteins in health and diseases. The global market for fluorescence-based products is estimated in billions of dollars p.a. The discovery of natural FPs from GBR with superior molecular, biophysical and optical characteristics to those of commercially available FPs offers a unique opportunity to develop advanced tools to study cellular processes and develop diagnostic assays for diseases such as cancer.Read moreRead less