Transport systems that underpin nitrogen efficient maize. This project aims to define the nitrogen transport network involved in the uptake, storage and redistribution of inorganic nitrogen (nitrate and ammonium) over the developmental life cycle of maize. This information will provide novel insight into the genetic control of nitrogen use in maize and other cereal crops.
What is the function of gamma-aminobutyric acid-gated anion channels in plants? The project will identify the molecular basis of gamma-aminobutyric acid (GABA) signalling in plants. This is significant because GABA regulates proteins that release molecules involved in root-soil interactions, growth, and fertilisation. The project's discoveries will allow improvement of these agronomic traits that ultimately determine crop yield.
Microgenomics - a tool to dissect effects of salinity on gene expression in specific cell types of Arabidopsis and rice. This project will provide novel, fundamental understanding of the cell type-specific processes involved in salinity tolerance in higher plants. As such, it will impact on our understanding of a range of processes relevant to salinity tolerance, an area of great importance to Australian agriculture and environmental sustainability. The increased understanding arising from this ....Microgenomics - a tool to dissect effects of salinity on gene expression in specific cell types of Arabidopsis and rice. This project will provide novel, fundamental understanding of the cell type-specific processes involved in salinity tolerance in higher plants. As such, it will impact on our understanding of a range of processes relevant to salinity tolerance, an area of great importance to Australian agriculture and environmental sustainability. The increased understanding arising from this project will underpin future work to increase agricultural productivity and the quality of life for all in the Australian and international communities.Read moreRead less
Characterisation of PQ loop proteins in plants: are they voltage insensitive nonselective cation channels? Millions of hectares of Australian agricultural land are affected by salinity. This results in the loss of hundreds of millions of dollars in revenue each year. The identification of the pathway for the initial influx of Na+ into plants from the soil will be important in helping to develop crop plants that are salt tolerant. This will increase our understanding of the mechanisms of salinity ....Characterisation of PQ loop proteins in plants: are they voltage insensitive nonselective cation channels? Millions of hectares of Australian agricultural land are affected by salinity. This results in the loss of hundreds of millions of dollars in revenue each year. The identification of the pathway for the initial influx of Na+ into plants from the soil will be important in helping to develop crop plants that are salt tolerant. This will increase our understanding of the mechanisms of salinity tolerance, an area of great importance to Australian agriculture and environmental sustainability. The future applications of this work will increase agricultural productivity and enhance the quality of life for both Australians and the international community.Read moreRead less
Finding the missing links in salt and water transport in plants. Grain crops and horticultural plants use proteins called aquaporins to move water across cell membranes, but a group of these proteins can also transport some important nutrient ions as well as toxic sodium ions. This project aims to reveal the molecular pathways that regulate water and ion transport via aquaporins using advanced techniques in biophysics and molecular biology. These results will provide novel insights into how plan ....Finding the missing links in salt and water transport in plants. Grain crops and horticultural plants use proteins called aquaporins to move water across cell membranes, but a group of these proteins can also transport some important nutrient ions as well as toxic sodium ions. This project aims to reveal the molecular pathways that regulate water and ion transport via aquaporins using advanced techniques in biophysics and molecular biology. These results will provide novel insights into how plants coordinate and adapt to changing water and salt conditions, addressing a missing link in how ions and water move in and out of plant vacuoles. Benefits include an expanded, innovative range of targets for plant breeding programs to improve plant productivity in our changing climate.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0775503
Funder
Australian Research Council
Funding Amount
$255,000.00
Summary
Robotics for plant genomics: Increasing throughput in plant genetic analyses. Plant genomics has direct benefit to crop improvement, especially as focussed in the applicants' laboratories. Thus, the Australian agri-food sector will benefit substantially from the acceleration in plant functional genomics that will arise from the installation of the robotics equipment described in the current application, by both underpinning more applied research and also being used directly in crop improvement p ....Robotics for plant genomics: Increasing throughput in plant genetic analyses. Plant genomics has direct benefit to crop improvement, especially as focussed in the applicants' laboratories. Thus, the Australian agri-food sector will benefit substantially from the acceleration in plant functional genomics that will arise from the installation of the robotics equipment described in the current application, by both underpinning more applied research and also being used directly in crop improvement programs such as are based at the Waite Campus. The outputs will include crops with increased tolerance to biotic and abiotic stresses, a reduced dependence on chemical inputs such as fertilisers and improved food quality, with consequent benefits to the environment and human health and nutrition.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0347358
Funder
Australian Research Council
Funding Amount
$478,000.00
Summary
Adelaide high-speed cell sorter and analyser facility. Cell sorting and flow cytometric analysis represent powerful and essential tools in modern cell and molecular biology. The applicants seek to develop a core facility within Adelaide University housing a high-speed Fluorescence Activated Cell Sorter and a high-speed flow cytometric analyser. Such a facility is not currently available to researchers in Adelaide. These high-speed machines will allow studies on rare cells and subcellular compon ....Adelaide high-speed cell sorter and analyser facility. Cell sorting and flow cytometric analysis represent powerful and essential tools in modern cell and molecular biology. The applicants seek to develop a core facility within Adelaide University housing a high-speed Fluorescence Activated Cell Sorter and a high-speed flow cytometric analyser. Such a facility is not currently available to researchers in Adelaide. These high-speed machines will allow studies on rare cells and subcellular components, currently not possible on other machines and meeting the demand for access to this sophisticated equipment is vital for cell and molecular biologists in Adelaide to continue advances in genomics and proteomics.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0668294
Funder
Australian Research Council
Funding Amount
$110,000.00
Summary
Isotope Ratio Mass Spectrometry Facility for Nitrogen and Water Analysis in Plants. Continual improvement to agricultural plant production is key to maintaining future sustainable growth in Australian agriculture. Our respective research teams are focussed on improving how plants utilise both nitrogen and water. Many questions remain with respect to where, how and when plants use and or access these important nutrients. The proposed facility will enable plant scientists to begin in-depth anal ....Isotope Ratio Mass Spectrometry Facility for Nitrogen and Water Analysis in Plants. Continual improvement to agricultural plant production is key to maintaining future sustainable growth in Australian agriculture. Our respective research teams are focussed on improving how plants utilise both nitrogen and water. Many questions remain with respect to where, how and when plants use and or access these important nutrients. The proposed facility will enable plant scientists to begin in-depth analysis of both nitrogen transport mechanisms and the ability to model root development and water allocation in crop species. This research will ultimately lead to improved knowledge on how plants respond to their environment and where modifications can be made to generate sustainable crops suited to Australian agriculture.Read moreRead less
IMPROVING NITROGEN USE EFFICIENCY IN CROP PLANTS: ROLE OF THE AMMONIUM TRANSPORT FAMILY AMT. Improving nitrogen use efficiency in crop plants will reduce the use of environmentally damaging nitrogen fertilisers that threaten through leaching the sustainability of Australia's agricultural sector and local water ecosystems. Plants contain genes that encode transport proteins required for the uptake of nitrogen (ammonium and nitrate) from the soil. We will identify the in planta activity of the A ....IMPROVING NITROGEN USE EFFICIENCY IN CROP PLANTS: ROLE OF THE AMMONIUM TRANSPORT FAMILY AMT. Improving nitrogen use efficiency in crop plants will reduce the use of environmentally damaging nitrogen fertilisers that threaten through leaching the sustainability of Australia's agricultural sector and local water ecosystems. Plants contain genes that encode transport proteins required for the uptake of nitrogen (ammonium and nitrate) from the soil. We will identify the in planta activity of the AMT family of ammonium transporters and associated signalling pathways which control the uptake and assimilation of ammonium in plants. This project will confirm the mechanisms involved in ammonium uptake from the soil and lead to the development of ammonium-nitrogen efficient crop plants.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE0561161
Funder
Australian Research Council
Funding Amount
$110,000.00
Summary
Joint Facility for Genome Analysis of Nutrient Transport Proteins. The joint facility for genome analysis of nutrient transport proteins is a new initiative between the University of Adelaide, the Australian Centre for Plant Functional Genomics, and the University of Western Australia to use a high throughput Xenopus oocyte expression system to screen plant cDNA/cRNA collections for genes encoding nutrient transport proteins. The facility will also provide a platform to rapidly accelerate our p ....Joint Facility for Genome Analysis of Nutrient Transport Proteins. The joint facility for genome analysis of nutrient transport proteins is a new initiative between the University of Adelaide, the Australian Centre for Plant Functional Genomics, and the University of Western Australia to use a high throughput Xenopus oocyte expression system to screen plant cDNA/cRNA collections for genes encoding nutrient transport proteins. The facility will also provide a platform to rapidly accelerate our present capacity for Xenopus oocyte expression analysis of nutrient transport proteins. This facility will greatly aid our current research quantum in this field and allow for new discoveries related to nutrient transport in plants.Read moreRead less