The evolutionary transition from anaerobic to aerobic metabolism. This project aims to find out how life on Earth survived the revolutionary changes when cyanobacteria first released oxygen into the atmosphere. These events led to a transition from anoxic (oxygen-free) to oxic (oxygen-rich) conditions. A comparative genomic view across a series of photosynthetic organisms will be performed at the molecular level with ecological interpretation. Understanding of what metabolic changes occurred in ....The evolutionary transition from anaerobic to aerobic metabolism. This project aims to find out how life on Earth survived the revolutionary changes when cyanobacteria first released oxygen into the atmosphere. These events led to a transition from anoxic (oxygen-free) to oxic (oxygen-rich) conditions. A comparative genomic view across a series of photosynthetic organisms will be performed at the molecular level with ecological interpretation. Understanding of what metabolic changes occurred in response to the shifts in the environment will have wide implications for predicting the evolutionary events that are still occurring today, such as rapidly changing climatic conditions. This fundamental research will enhance Australia's profile in this field.Read moreRead less
Modern reptiles with ancient toxins: the molecular origin and evolution of novel bioactive proteins from squamate dental glands. Animal venoms provide a rich source of novel bioactive proteins, some of which have demonstrated therapeutically useful activities. Through this researcher's unique approach of investigating previously unmapped squamate venom systems, there is potential for the identification of divergent, bioactive proteins. Those already identified by the applicant in the dental gl ....Modern reptiles with ancient toxins: the molecular origin and evolution of novel bioactive proteins from squamate dental glands. Animal venoms provide a rich source of novel bioactive proteins, some of which have demonstrated therapeutically useful activities. Through this researcher's unique approach of investigating previously unmapped squamate venom systems, there is potential for the identification of divergent, bioactive proteins. Those already identified by the applicant in the dental glands of Australian monitor lizard species represent a tremendous opportunity for biodiscovery. Further knowledge in this area will increase medical understandings of bites and aid conservation measures informed by the natural history of these animals.Read moreRead less
Taming the intruders: the domestication of Tigger transposable elements in mammals. It has become apparent that most of the DNA that makes us what we are is actually comprised of the remnants of invading parasitic DNA acquired over time. A continual battle exists between host which tries to silence or remove this DNA, and the parasite that tries to multiply and spread. We are currently investigating an intriguing aspect of this process that involves host genomes 'domesticating' parasitic DNA to ....Taming the intruders: the domestication of Tigger transposable elements in mammals. It has become apparent that most of the DNA that makes us what we are is actually comprised of the remnants of invading parasitic DNA acquired over time. A continual battle exists between host which tries to silence or remove this DNA, and the parasite that tries to multiply and spread. We are currently investigating an intriguing aspect of this process that involves host genomes 'domesticating' parasitic DNA to provide novel functions, thereby facilitating the evolution of specific characteristics within species.Read moreRead less
Using venoms to map critical and evolutionary conserved vulnerabilities. We have developed and applied new functional genomic approaches to study venom evolution. Using CRISPR screening, we find that unrelated venoms act on cells by exploiting the same vulnerabilities. By functionally mapping these vulnerabilities for all venom classes, we can begin to develop universal venom antidotes. Conversely, much of what we know about venom mechanisms comes from a small percentage of the biodiversity with ....Using venoms to map critical and evolutionary conserved vulnerabilities. We have developed and applied new functional genomic approaches to study venom evolution. Using CRISPR screening, we find that unrelated venoms act on cells by exploiting the same vulnerabilities. By functionally mapping these vulnerabilities for all venom classes, we can begin to develop universal venom antidotes. Conversely, much of what we know about venom mechanisms comes from a small percentage of the biodiversity within a venom, and we have developed genomic tools to study the venom “dark matter”. This work will lead to the full molecular characterisation of venom biodiversity, and new venom components will be useful for research or as novel medicines.Read moreRead less
Evolution of the biofabrication of mineralized structures in animals. Shells and skeletons are produced by a wide range of animals. These highly-order crystalline structures are genetically-encoded and produce high-performance composite materials that exceed present capabilities in human engineering. This international collaboration will elucidate the molecular mechanisms controlling the fabrication of these architectures. This knowledge will contribute significantly to the development of materi ....Evolution of the biofabrication of mineralized structures in animals. Shells and skeletons are produced by a wide range of animals. These highly-order crystalline structures are genetically-encoded and produce high-performance composite materials that exceed present capabilities in human engineering. This international collaboration will elucidate the molecular mechanisms controlling the fabrication of these architectures. This knowledge will contribute significantly to the development of materials for advanced electronics and energy transducers, human bone therapeutics and marine-based products such as pearls and cements, through the identification of genes underlying biofabrication networks and the development of in vitro bioproduction systems.Read moreRead less
Evolution and ecology of integron gene cassettes: exploring the protein universe. Bacteria rapidly adapt to new conditions by sharing diverse genes via lateral genetic transfer, best illustrated by the spread of antibiotic resistance. This study will characterise mobile genes, discovering new gene families and proteins, and will expand existing knowledge of bacterial evolution.
Function and evolution of insect odorant receptors. This project aims to shed light on how insect odorant receptors function by using comparative genomic studies between the genetic model insect Drosophila melanogaster and a pest species, the Australian sheep blowfly. This project expects to generate knowledge of how specific chemicals activate specific receptors in order to excite sensory neurons and drive behaviour, which is not well understood. Expected outcomes include increased understandin ....Function and evolution of insect odorant receptors. This project aims to shed light on how insect odorant receptors function by using comparative genomic studies between the genetic model insect Drosophila melanogaster and a pest species, the Australian sheep blowfly. This project expects to generate knowledge of how specific chemicals activate specific receptors in order to excite sensory neurons and drive behaviour, which is not well understood. Expected outcomes include increased understanding of olfaction in insects, increased national and international collaboration, and outstanding graduate student training. This research will be of significant future benefit in deriving methods to modify the behaviour of insects of agricultural or medical importance, for example the sheep blowfly. Read moreRead less
The genomics of adaptation in Wolbachia pipientis, an emerging biocontrol agent. Australians are increasingly exposed to insect-transmitted diseases such as dengue fever. Novel biocontrol methods using the bacterium Wolbachia aim to control insect populations to reduce disease transmission. Our research will be the first to investigate genomic variation and the process of adaptation to new insect hosts in Wolbachia. The novel data and understanding of evolutionary processes we generate will be c ....The genomics of adaptation in Wolbachia pipientis, an emerging biocontrol agent. Australians are increasingly exposed to insect-transmitted diseases such as dengue fever. Novel biocontrol methods using the bacterium Wolbachia aim to control insect populations to reduce disease transmission. Our research will be the first to investigate genomic variation and the process of adaptation to new insect hosts in Wolbachia. The novel data and understanding of evolutionary processes we generate will be critical for screening bacterial biocontrol candidates and designing biocontrol release strategies. It will also strengthen the position of Australian research as a world-leader in the fusion of post-genomics and applied microbiology. Read moreRead less
Origin of multicellularity in animals: identification and analysis of intercellular signalling pathways in a basal metazoan, the demosponge Reniera. The Reniera genome project is a multi-million dollar collaboration between JGI (US-DOE) and Australian scientists that will see the sequencing of the first Australian marine animal by 2006. This project will significantly advance our understanding of the origins of animals and contribute to the reconstruction of creatures that lived over 600 million ....Origin of multicellularity in animals: identification and analysis of intercellular signalling pathways in a basal metazoan, the demosponge Reniera. The Reniera genome project is a multi-million dollar collaboration between JGI (US-DOE) and Australian scientists that will see the sequencing of the first Australian marine animal by 2006. This project will significantly advance our understanding of the origins of animals and contribute to the reconstruction of creatures that lived over 600 million years ago. A major outcome of this reconstruction will be a fundamental understanding of how cells communicate with each other during the process of development to give rise to the diversity of cell types within multicellular animals. This study will also shed light on what happens when cell communication goes astray, as observed in a range of human malignancies, including cancer. Read moreRead less
The sponge genome project and the evolution of multicellularity: using comparative genomics and developmental biology to reconstruct the first animals. Recently the entire genome from a living fossil - a sponge from the Great Barrier Reef - was sequenced (jointly supported by the ARC and US Department of Energy). As this genome is assembled and analysed, many of the fundamental biological processes that underlie the construction and evolution of all animals, including humans, will be revealed. ....The sponge genome project and the evolution of multicellularity: using comparative genomics and developmental biology to reconstruct the first animals. Recently the entire genome from a living fossil - a sponge from the Great Barrier Reef - was sequenced (jointly supported by the ARC and US Department of Energy). As this genome is assembled and analysed, many of the fundamental biological processes that underlie the construction and evolution of all animals, including humans, will be revealed. In addition, sponge genomics will fuel innovations in medicine and biotechnology. Specifically, sponges are renowned for their capacity to synthesise bioactive compounds used in drug development, and high-grade silica used for semi-conductor construction. This project will identify the gene networks controlling these biosynthetic processes.Read moreRead less