How plants open up: revealing the evolution of stomatal opening mechanisms. This project aims to identify novel and conserved mechanisms that drive the opening of stomata – plant pores that enable CO2 acquisition for photosynthesis. Stomatal movements strongly affect plant productivity and water use efficiency and have profoundly influenced the earth’s climate and terrestrial ecology. This project will address critical gaps in our understanding of how plants open stomata in response to their env ....How plants open up: revealing the evolution of stomatal opening mechanisms. This project aims to identify novel and conserved mechanisms that drive the opening of stomata – plant pores that enable CO2 acquisition for photosynthesis. Stomatal movements strongly affect plant productivity and water use efficiency and have profoundly influenced the earth’s climate and terrestrial ecology. This project will address critical gaps in our understanding of how plants open stomata in response to their environment and the evolutionary history of the genes controlling this fundamental process. A major expected outcome is knowledge of the diversity of stomatal opening pathways, which should ultimately lead to improved predictions of plant responses to environmental change and assist future targeted modification of plant growth.Read moreRead less
Discovery Early Career Researcher Award - Grant ID: DE200101133
Funder
Australian Research Council
Funding Amount
$427,067.00
Summary
The quick and the dead: identifying mechanisms for plant drought survival. This project aims to identify genes that control plant responses to low air humidity, which enhance drought survival by restricting water loss. Most plant water loss occurs through pores called stomata. This project expects to identify the genes that close stomata within minutes of decreased humidity by determining the molecular changes that occur over this timeframe and testing candidate genes for a critical role. Divers ....The quick and the dead: identifying mechanisms for plant drought survival. This project aims to identify genes that control plant responses to low air humidity, which enhance drought survival by restricting water loss. Most plant water loss occurs through pores called stomata. This project expects to identify the genes that close stomata within minutes of decreased humidity by determining the molecular changes that occur over this timeframe and testing candidate genes for a critical role. Diverse land plant models will be examined to ensure broad applicability of results. A major expected outcome is new knowledge of genes that minimise plant water loss, which would ultimately benefit plant-based industries through new targets for breeding improved, drought-adapted varieties for food security in a drying climate.Read moreRead less
The evolution of specialised orchid pollination and its reversibility. This project aims to determine the changes in key floral volatile compounds underpinning pollination transitions, identify their molecular basis, and understand the ecological processes favouring reversals away from extreme specialisation. By focusing on pollination of sexually deceptive Australian orchids, this project would be the first to determine the molecular, chemical and behavioural basis of evolutionary reversals to ....The evolution of specialised orchid pollination and its reversibility. This project aims to determine the changes in key floral volatile compounds underpinning pollination transitions, identify their molecular basis, and understand the ecological processes favouring reversals away from extreme specialisation. By focusing on pollination of sexually deceptive Australian orchids, this project would be the first to determine the molecular, chemical and behavioural basis of evolutionary reversals to more generalised strategies in a group of plants facing high risk of pollinator extinction. The expected outcome, a mechanistic understanding of how pollination transitions occur, would be internationally ground-breaking, and provide crucial insights to protect this diverse but highly threatened group of plants.Read moreRead less
Evolution of defensive and predatory venom in cone snails. This project aims to determine the molecular and cellular origins and mechanisms regulating venom production and release to establish how defensive venoms evolved in cone snails. Cone snails possess a remarkable ability to rapidly and reversibly switch between separate venoms in response to predatory or defensive stimuli, implying that these are separately evolved and regulated mechanisms. The investigators hypothesise that defensive ven ....Evolution of defensive and predatory venom in cone snails. This project aims to determine the molecular and cellular origins and mechanisms regulating venom production and release to establish how defensive venoms evolved in cone snails. Cone snails possess a remarkable ability to rapidly and reversibly switch between separate venoms in response to predatory or defensive stimuli, implying that these are separately evolved and regulated mechanisms. The investigators hypothesise that defensive venoms, originally evolved in the proximal venom duct to protect against threats such as cephalopod and fish predation, have been repurposed in the proximal duct to allow predators to become prey, facilitating the switch from worm to mollusc and fish hunting. The project aims to show the broad implications for the evolution of venoms in animals and discover the regulatory mechanisms driving venom peptide expression.Read moreRead less
Revealing novel mechanisms conferring evolution of resistance to glufosinate and glyphosate in Eleusine indica. Glyphosate and its alternative glufosinate are the most important herbicides in world agriculture. The world’s first cases of glufosinate resistance in Eleusine indica have been recently reported. The aims of the proposed research is to identify the currently unknown biochemical and molecular mechanisms conferring glufosinate resistance, to unravel the novel molecular mechanism endowin ....Revealing novel mechanisms conferring evolution of resistance to glufosinate and glyphosate in Eleusine indica. Glyphosate and its alternative glufosinate are the most important herbicides in world agriculture. The world’s first cases of glufosinate resistance in Eleusine indica have been recently reported. The aims of the proposed research is to identify the currently unknown biochemical and molecular mechanisms conferring glufosinate resistance, to unravel the novel molecular mechanism endowing very high level glyphosate resistance, and to elucidate the evolutionary trajectory of glyphosate resistance in E. indica. This will advance our current knowledge and understanding of resistance evolution and have impact on resistance management.Read moreRead less
Gene regulation by retroelement encoded natural antisense transcripts. Genetic information underpins all life on earth and is processed to make proteins, which determine the characteristics of an organism. However, only about 2% of our whole genome is made up of genes that encode proteins; the other 98% is non-coding and its function remains poorly understood. Aims and Significance: This proposal aims to utilise cutting edge genomic technologies to generate new knowledge about how the non-coding ....Gene regulation by retroelement encoded natural antisense transcripts. Genetic information underpins all life on earth and is processed to make proteins, which determine the characteristics of an organism. However, only about 2% of our whole genome is made up of genes that encode proteins; the other 98% is non-coding and its function remains poorly understood. Aims and Significance: This proposal aims to utilise cutting edge genomic technologies to generate new knowledge about how the non-coding genome regulates the expression of protein coding genes. Expected Outcomes and Benefits: This proposal will provide novel targets and methodology for gene modulation with broad applications from biology to environmental sciences.Read moreRead less
Imaging the world of miniature venomous arthropods. Venomous arthropods produce a myriad of biologically active peptides, with many having potential as pharmacological tools, bioinsecticides and pharmaceuticals. Most studies to date have focussed on large arthropods; smaller species remain neglected due to the difficulties of venom collection. This project seeks to further advance the pioneering imaging mass spectrometry approaches the project team developed for imaging toxins in the venom gland ....Imaging the world of miniature venomous arthropods. Venomous arthropods produce a myriad of biologically active peptides, with many having potential as pharmacological tools, bioinsecticides and pharmaceuticals. Most studies to date have focussed on large arthropods; smaller species remain neglected due to the difficulties of venom collection. This project seeks to further advance the pioneering imaging mass spectrometry approaches the project team developed for imaging toxins in the venom glands of spiders and centipedes. By combining high-resolution matrix-assisted laser desorption ionisation imaging data with histological and transcriptomic information the project aims to provide the first detailed insights into the neglected world of miniature arthropod venoms. The approaches developed by this project aim to have wide application in the field of biology.Read moreRead less
The nature and consequences of environmentally-generated phenotypic variation in natural populations. The ambient environment can generate both heritable and non-heritable variation in individual traits, but the role of such variation in evolution is poorly understood. This project will use a powerful model organism, the Australian neriid flies, to elucidate the evolutionary implications of environmentally-generated variation.
Understanding trifluralin resistance in annual ryegrass, a major Australian agricultural weed. This project aims to better understand the biochemical and molecular basis of trifluralin resistance in annual ryegrass. The herbicide trifluralin is an alternative to soil cultivation in controlling crop-infesting weeds such as annual ryegrass. However, resistance to trifluralin in annual ryegrass now looms. A fundamental understanding and insight into trifluralin resistance will assist resistance man ....Understanding trifluralin resistance in annual ryegrass, a major Australian agricultural weed. This project aims to better understand the biochemical and molecular basis of trifluralin resistance in annual ryegrass. The herbicide trifluralin is an alternative to soil cultivation in controlling crop-infesting weeds such as annual ryegrass. However, resistance to trifluralin in annual ryegrass now looms. A fundamental understanding and insight into trifluralin resistance will assist resistance management, trifluralin sustainability and soil conservation. This will provide significant benefits for Australian grain production and soil conservation.Read moreRead less
Conservation genomics of a critically endangered insect. This project aims to develop tools genotyping large animal genomes, focusing on the case of the Lord Howe Island stick insect, once thought to be extinct and now critically endangered. This project expects to generate molecular tools to monitor the genetic health the insect which has a large, complex and poorly understood genome. Expected outcomes include the development of a preservation and reintroduction strategy for the insect. This pr ....Conservation genomics of a critically endangered insect. This project aims to develop tools genotyping large animal genomes, focusing on the case of the Lord Howe Island stick insect, once thought to be extinct and now critically endangered. This project expects to generate molecular tools to monitor the genetic health the insect which has a large, complex and poorly understood genome. Expected outcomes include the development of a preservation and reintroduction strategy for the insect. This project will benefit ongoing conservation efforts, and is timely given the ongoing eradication of rats from Lord Howe Island where this species once lived. Read moreRead less