Linkage Infrastructure, Equipment And Facilities - Grant ID: LE180100157
Funder
Australian Research Council
Funding Amount
$600,000.00
Summary
Confocal and single molecule microscopes for systems microscopy. This project aims to establish Australia’s first system microscopy facility with dedicated live-cell confocal and single-molecule fluorescence microscopes. In systems microscopy, the imaging workflow is automated so that large and unbiased data sets of the spatiotemporal organisation of molecules and cells can be generated. Combined with statistical and bioinformatics analyses, image-derived data provides system-wide information th ....Confocal and single molecule microscopes for systems microscopy. This project aims to establish Australia’s first system microscopy facility with dedicated live-cell confocal and single-molecule fluorescence microscopes. In systems microscopy, the imaging workflow is automated so that large and unbiased data sets of the spatiotemporal organisation of molecules and cells can be generated. Combined with statistical and bioinformatics analyses, image-derived data provides system-wide information that is not easily obtainable with other approaches. The project will enable Australian researchers to image and analyse the full complexity of biological systems, potentially transforming cell biology, drug development and understanding the molecular basis of disease. It will also demonstrate how the capacity of microscopy facilities can be enhanced and bias in imaging data reduced by automating data acquisition and mining of image-based data.Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE150100163
Funder
Australian Research Council
Funding Amount
$560,000.00
Summary
Single molecule imaging laboratory. Single molecule imaging laboratory: The goal of the project is to establish a single molecule imaging laboratory to close the gap between structural imaging and cellular imaging. Utilising the expertise of the ARC Centre of Excellence in Advanced Molecular Imaging, the aim of the project is to design, build and apply three microscopes that go beyond the current commercial solutions for single molecule localisation microscopy such as Photo-Activation Localisati ....Single molecule imaging laboratory. Single molecule imaging laboratory: The goal of the project is to establish a single molecule imaging laboratory to close the gap between structural imaging and cellular imaging. Utilising the expertise of the ARC Centre of Excellence in Advanced Molecular Imaging, the aim of the project is to design, build and apply three microscopes that go beyond the current commercial solutions for single molecule localisation microscopy such as Photo-Activation Localisation Microscopy (PALM) and Stochastic Optical Reconstruction Microscopy (STORM) and perform single molecule imaging: deep inside cells and tissue.The facility will have a fast acquisition rate to monitor highly dynamic molecular events, and improved precision to image molecules and complexes in intact cells with less than or equal to one nanometre resolution. There is currently no comparable imaging facility in the world.Read moreRead less