Linkage Infrastructure, Equipment And Facilities - Grant ID: LE210100046
Funder
Australian Research Council
Funding Amount
$289,381.00
Summary
A fast fluorescence lifetime imaging microscope to track protein dynamics. This project aims to establish a fast fluorescence lifetime imaging microscope that can track the intracellular journey of a protein throughout the entire structural framework of a living cell. By coupling single particle tracking technology with a cutting-edge fluorescence lifetime camera, this one-of-a-kind microscope will enable protein mobility and interaction to be spatially mapped with unprecedented temporal resolut ....A fast fluorescence lifetime imaging microscope to track protein dynamics. This project aims to establish a fast fluorescence lifetime imaging microscope that can track the intracellular journey of a protein throughout the entire structural framework of a living cell. By coupling single particle tracking technology with a cutting-edge fluorescence lifetime camera, this one-of-a-kind microscope will enable protein mobility and interaction to be spatially mapped with unprecedented temporal resolution. The benefit of this technology is that it will enable scientists in Australia to image, for the first time, the biophysical mechanism by which a protein navigates intracellular architecture to regulate a complex biological function at the single molecule level.Read moreRead less
Photosynthesis under extreme conditions. The aim of this project is to characterise modifications to the light dependent reactions of photosynthesis of simple, single cell organisms that live under harsh environmental conditions including: i) elevated temperature; ii) low, variable and low energy (red) light; iii) arid and variable hydration; and iv) chemical stress e.g. low pH. In a changing biosphere brought about by anthropological climate change, a better understanding of existing adaptions ....Photosynthesis under extreme conditions. The aim of this project is to characterise modifications to the light dependent reactions of photosynthesis of simple, single cell organisms that live under harsh environmental conditions including: i) elevated temperature; ii) low, variable and low energy (red) light; iii) arid and variable hydration; and iv) chemical stress e.g. low pH. In a changing biosphere brought about by anthropological climate change, a better understanding of existing adaptions of bacterial photosynthetic organisms may allow more resilient crops and other essential plants to be developed in the future. The project brings together an international consortium of world renowned experts across key aspects of photosynthesis. Read moreRead less
Molecular mechanisms of mechanosensation and shape regulation in cells. This project aims to explore how cells physically sense and respond to the surrounding environment on a molecular level. Physical distortion of erythrocytes doubles their glucose consumption and increases cation membrane flux five-fold. This mechanism involves opening of the mechanosenstive ion channel Piezo1. This project will include a kinetic description of these phenomena, with a goal to establish a predictive mathematic ....Molecular mechanisms of mechanosensation and shape regulation in cells. This project aims to explore how cells physically sense and respond to the surrounding environment on a molecular level. Physical distortion of erythrocytes doubles their glucose consumption and increases cation membrane flux five-fold. This mechanism involves opening of the mechanosenstive ion channel Piezo1. This project will include a kinetic description of these phenomena, with a goal to establish a predictive mathematical model of the regulation of cell-shape and volume. The project will provide an understanding of mechanisms operating when cells and tissues are succumbing to trauma and invasion, and how to control these processes on a molecular level.Read moreRead less