Linkage Infrastructure, Equipment And Facilities - Grant ID: LE150100066
Funder
Australian Research Council
Funding Amount
$440,000.00
Summary
Mass Cytometry: A breakthrough in multidimensional systems biology. Mass cytometry - a breakthrough in multidimensional systems biology: Mass Cytometry by Time of Flight marries the resolution, specificity and sensitivity of atomic stable isotope mass spectrometry to the high-throughput, single-cell analytical advantages of flow cytometry. Using molecular probes conjugated with stable isotope tags, a large increase is possible in the number of simultaneous quantitative measurements in complex sa ....Mass Cytometry: A breakthrough in multidimensional systems biology. Mass cytometry - a breakthrough in multidimensional systems biology: Mass Cytometry by Time of Flight marries the resolution, specificity and sensitivity of atomic stable isotope mass spectrometry to the high-throughput, single-cell analytical advantages of flow cytometry. Using molecular probes conjugated with stable isotope tags, a large increase is possible in the number of simultaneous quantitative measurements in complex samples. These parameters, denoting cell type, function and signalling status, will make possible future advances in the understanding of the diversity of cell phenotype and function with a systems biology approach. Read moreRead less
Linkage Infrastructure, Equipment And Facilities - Grant ID: LE180100157
Funder
Australian Research Council
Funding Amount
$600,000.00
Summary
Confocal and single molecule microscopes for systems microscopy. This project aims to establish Australia’s first system microscopy facility with dedicated live-cell confocal and single-molecule fluorescence microscopes. In systems microscopy, the imaging workflow is automated so that large and unbiased data sets of the spatiotemporal organisation of molecules and cells can be generated. Combined with statistical and bioinformatics analyses, image-derived data provides system-wide information th ....Confocal and single molecule microscopes for systems microscopy. This project aims to establish Australia’s first system microscopy facility with dedicated live-cell confocal and single-molecule fluorescence microscopes. In systems microscopy, the imaging workflow is automated so that large and unbiased data sets of the spatiotemporal organisation of molecules and cells can be generated. Combined with statistical and bioinformatics analyses, image-derived data provides system-wide information that is not easily obtainable with other approaches. The project will enable Australian researchers to image and analyse the full complexity of biological systems, potentially transforming cell biology, drug development and understanding the molecular basis of disease. It will also demonstrate how the capacity of microscopy facilities can be enhanced and bias in imaging data reduced by automating data acquisition and mining of image-based data.Read moreRead less