Maximising pineapple production for Australian farmers using genome editing. This project addresses the single biggest issue affecting the viability of pineapple farming in Australia and internationally: premature flowering leading to supply collapse. We aim to develop CRISPR technology to breed new pineapple varieties using non-GM approaches. Expected outcomes include the production of pineapples with resistance to premature flowering, as well as the technologies to deliver additional improveme ....Maximising pineapple production for Australian farmers using genome editing. This project addresses the single biggest issue affecting the viability of pineapple farming in Australia and internationally: premature flowering leading to supply collapse. We aim to develop CRISPR technology to breed new pineapple varieties using non-GM approaches. Expected outcomes include the production of pineapples with resistance to premature flowering, as well as the technologies to deliver additional improvements in the future. The new varieties will benefit farmers through increased production, maximising supply capability for a rapidly growing internal demand. This will benefit Australian pineapple producers through higher profitability in existing markets, as well as enabling expansion into international and future markets.Read moreRead less
Understanding, controlling and improving the flavour of almond kernels. Almond kernels may be sweet, semi-bitter or bitter, with the first two categories marketed as fresh nuts, while the latter are used in processed products such as marzipan. Semi-bitter kernels have a more interesting flavour than sweet kernels and we require tools to breed for this character. The bitter flavour is imparted by amygdalin via the cyanogenic pathway. This project will characterise the genetic control of sweet, ....Understanding, controlling and improving the flavour of almond kernels. Almond kernels may be sweet, semi-bitter or bitter, with the first two categories marketed as fresh nuts, while the latter are used in processed products such as marzipan. Semi-bitter kernels have a more interesting flavour than sweet kernels and we require tools to breed for this character. The bitter flavour is imparted by amygdalin via the cyanogenic pathway. This project will characterise the genetic control of sweet, semi-bitter and bitter flavour, amygdalin accumulation in developing kernels, and key enzymes in the cyanogenic pathway. Almond populations segregating for these traits will be used and the data will be integrated into the Australian almond meiotic map.Read moreRead less
Microsatellite marker development for almond breeding. Australian almond growers produce 8,000 tonnes of kernel annually, and the long term aim is to develop strong export markets. The industry funds a breeding program at Adelaide University, comprising hybridisation and selection. Through our current ARC SPIRT grant we augment this with a program in molecular techniques. Research is well-advanced in the development of a genetic map for almond based on both molecular and agronomic characters. ....Microsatellite marker development for almond breeding. Australian almond growers produce 8,000 tonnes of kernel annually, and the long term aim is to develop strong export markets. The industry funds a breeding program at Adelaide University, comprising hybridisation and selection. Through our current ARC SPIRT grant we augment this with a program in molecular techniques. Research is well-advanced in the development of a genetic map for almond based on both molecular and agronomic characters. This IREX grant will facilitate integration of our map with the International Prunus mapping program, based at Cabrils, Spain. It will also facilitate augmentation of the map with jointly-developed microsatellite markers.Read moreRead less
Molecular tools for understanding, predicting and managing flowering and reproductive development in Brassica oleracea. This project integrates basic research with application to a significant horticultural industry, building on Australia's position as a leader in world research on molecular and genetic regulation of flowering. The project will strengthen Australia's research reputation in this field, provide new tools for cultivar screening in and management of B. oleracea seed crops. The proj ....Molecular tools for understanding, predicting and managing flowering and reproductive development in Brassica oleracea. This project integrates basic research with application to a significant horticultural industry, building on Australia's position as a leader in world research on molecular and genetic regulation of flowering. The project will strengthen Australia's research reputation in this field, provide new tools for cultivar screening in and management of B. oleracea seed crops. The project will deliver training for 3 PhD students at the interface between basic and applied research. A shortage of skilled scientists with ability to link understanding of plant development at the molecular/genetic level with improved crop management practices has been identified by the horticultural sector as a major challenge. Read moreRead less
Improved olive productivity and the use of molecular markers. Sales of olive oil on international markets are growing at 15% per year, and the Australian industry is expanding rapidly to win some of this trade. Varietal identity is a major problem, which has production implications and this project will verify trueness-to type via genetic fingerprinting. Many olive cultivars require cross pollination for optimal production, and the research will identify compatible cultivars for the major variet ....Improved olive productivity and the use of molecular markers. Sales of olive oil on international markets are growing at 15% per year, and the Australian industry is expanding rapidly to win some of this trade. Varietal identity is a major problem, which has production implications and this project will verify trueness-to type via genetic fingerprinting. Many olive cultivars require cross pollination for optimal production, and the research will identify compatible cultivars for the major varieties. Molecular markers will be developed to accelerate the selection of improved varieties with self-fertility, high oleic acid, low linolenic acid and tolerance to Peacock spot disease.Read moreRead less
The role of phytotoxins in ray blight disease of pyrethrum and significance of toxin tolerance to disease resistance. Ray blight disease, caused by Phoma ligulicola, is the greatest threat to the Australian pyrethrum industry causing $2.0M in annual losses. We aim to clarify the pathology of P.ligulicola in pyrethrum, focussing on the role of fungal toxins during infection. Toxin importance in disease will be determined through histology and induction of toxin- pathogen mutants. Toxins will be ....The role of phytotoxins in ray blight disease of pyrethrum and significance of toxin tolerance to disease resistance. Ray blight disease, caused by Phoma ligulicola, is the greatest threat to the Australian pyrethrum industry causing $2.0M in annual losses. We aim to clarify the pathology of P.ligulicola in pyrethrum, focussing on the role of fungal toxins during infection. Toxin importance in disease will be determined through histology and induction of toxin- pathogen mutants. Toxins will be characterised and used in tissue culture to select obtain toxin tolerant pyrethrum clones that will be evaluated for agronomic performance and disease resistance. Successful development of resistant clones will decrease production costs, minimise risk of fungicide resistance development and reduce environmental pollution.Read moreRead less
Factors responsible for host resistance to the pathogen Sclerotinia sclerotiorum for developing effective disease management in vegetable brassicas. Through successful identification of host resistance in vegetable Brassicas to Sclerotinia sclerotiorum and determining the factors associated with this resistance, breeders, for the first time, will be able to develop varieties with resistance against this difficult-to-manage pathogen. Benefits include prevention of severe losses in vegetable Bras ....Factors responsible for host resistance to the pathogen Sclerotinia sclerotiorum for developing effective disease management in vegetable brassicas. Through successful identification of host resistance in vegetable Brassicas to Sclerotinia sclerotiorum and determining the factors associated with this resistance, breeders, for the first time, will be able to develop varieties with resistance against this difficult-to-manage pathogen. Benefits include prevention of severe losses in vegetable Brassicas from Sclerotinia, and more viable and sustainable production with less reliance upon fungicides and toxic or ineffective fumigants. This research addresses the National Research Priority, an environmentally sustainable Australia, and the priority goal transforming existing industries, and will particularly benefit Australian horticultural communities.Read moreRead less
Manipulation of apoptosis-related genes to generate novel disease resistances in banana. Bananas are one of Australia's most important fruit crops. However, the industry is continually threatened by numerous diseases including yellow and black Sigatoka, Fusarium wilt and bunchy top. Control of these diseases currently requires either extensive pesticide treatments or complex agronomic procedures, of which most are ineffective, expensive or environmentally damaging. The aim of this project is to ....Manipulation of apoptosis-related genes to generate novel disease resistances in banana. Bananas are one of Australia's most important fruit crops. However, the industry is continually threatened by numerous diseases including yellow and black Sigatoka, Fusarium wilt and bunchy top. Control of these diseases currently requires either extensive pesticide treatments or complex agronomic procedures, of which most are ineffective, expensive or environmentally damaging. The aim of this project is to utilise a novel strategy to generate transgenic bananas with resistance to these diseases. The benefit will be both economic and environmental as implementation should significantly reduce or eliminate pesticide use. Importantly, this strategy can potentially be extrapolated to many other crops.Read moreRead less
Generating broad and stable resistance to gemini- and nanoviruses. The ssDNA geminiviruses and nanoviruses are considered a major threat to crop production in the tropics and subtropics. There are currently no effective control strategies for these viruses. We have been developing a novel transgenic strategy for generating resistance to ssDNA viruses based on the activation of a suicide gene in the presence of the viral Rep protein. Importantly, we have demonstrated proof-of-principle using Toba ....Generating broad and stable resistance to gemini- and nanoviruses. The ssDNA geminiviruses and nanoviruses are considered a major threat to crop production in the tropics and subtropics. There are currently no effective control strategies for these viruses. We have been developing a novel transgenic strategy for generating resistance to ssDNA viruses based on the activation of a suicide gene in the presence of the viral Rep protein. Importantly, we have demonstrated proof-of-principle using Tobacco yellow dwarf mastrevirus. In this project, we aim to further develop this strategy into a broad and stable mechanism for generating resistance to all known geminiviruses and nanoviruses.Read moreRead less