Miniaturised biosensors with high selectivity . This project aims to develop a technological platform for the fabrication of miniaturised and flexible sensors that enable the quantitative detection of important bioactive compounds such as fatty acids and biogenic amines. By utilising multi-enzymatic reactions in solid phase and engineering task-specific inks, chemiresistive sensors will be printed seamlessly as a whole. The sensors will respond to complex target biomolecules via a series of enzy ....Miniaturised biosensors with high selectivity . This project aims to develop a technological platform for the fabrication of miniaturised and flexible sensors that enable the quantitative detection of important bioactive compounds such as fatty acids and biogenic amines. By utilising multi-enzymatic reactions in solid phase and engineering task-specific inks, chemiresistive sensors will be printed seamlessly as a whole. The sensors will respond to complex target biomolecules via a series of enzymatic reactions through which the analyte will convert to much simpler, reactive and hence measurable molecules. This project will enable to design miniaturised sensors for point-of-care detection of biomolecules that cannot be yet evaluated by the end users.Read moreRead less
Enhancing biopharmaceuticals: A disruptive bioseparation resin technology. This project aims to develop an innovative and disruptive platform technology for designing and manufacturing tailor-made high-performance bioseparation resins to enhance biopharmaceuticals manufacturing. Bacterial cell factories will be developed to enable biotechnological production of innovative polyester bead-based bioseparation resins, which will revolutionise manufacturing of biopharmaceuticals. Expected outcomes o ....Enhancing biopharmaceuticals: A disruptive bioseparation resin technology. This project aims to develop an innovative and disruptive platform technology for designing and manufacturing tailor-made high-performance bioseparation resins to enhance biopharmaceuticals manufacturing. Bacterial cell factories will be developed to enable biotechnological production of innovative polyester bead-based bioseparation resins, which will revolutionise manufacturing of biopharmaceuticals. Expected outcomes of this project are cost-effective and strongly enhanced approaches for biopharmaceuticals recovery, thereby providing significant benefits to accelerate research and development in early stage discovery and manufacture of biologics, therapeutic proteins and vaccines.Read moreRead less
Efficient CO and CO2 conversion to biopolymers using phototrophic bacteria. This project aims to deliver efficient processes for the large-scale production of biopolymers from low cost inputs, using phototrophic bacteria. Feedstocks include syngas from solid wastes and carbon-dioxide-hydrogen mixes from fossil and renewable sources. The choice of phototrophic bacteria avoids the energy losses associated with existing technologies, since photons are used instead of chemical energy for metabolic n ....Efficient CO and CO2 conversion to biopolymers using phototrophic bacteria. This project aims to deliver efficient processes for the large-scale production of biopolymers from low cost inputs, using phototrophic bacteria. Feedstocks include syngas from solid wastes and carbon-dioxide-hydrogen mixes from fossil and renewable sources. The choice of phototrophic bacteria avoids the energy losses associated with existing technologies, since photons are used instead of chemical energy for metabolic needs. This project enables the production and optimisation of biopolymers through collaborations between engineers, polymer scientists and molecular biologists. The project will deliver technologies to produce tough, flexible and affordable biopolymers, converting wastes and greenhouse gases to a valuable product.Read moreRead less
Algal direct-air CO2 capture through interfacial enzyme immobilisation . Capturing CO2 directly from the atmosphere is challenging due to inherently slow mass transfer kinetics. This project aims to overcome this using an enzyme that can rapidly solubilise CO2 from air into water, to produce algae. By engineering the enzyme immobilisation at the air-water interface, this project will activate and protect the enzymes, increasing their lifespan and reducing costs. By understanding mass transfer an ....Algal direct-air CO2 capture through interfacial enzyme immobilisation . Capturing CO2 directly from the atmosphere is challenging due to inherently slow mass transfer kinetics. This project aims to overcome this using an enzyme that can rapidly solubilise CO2 from air into water, to produce algae. By engineering the enzyme immobilisation at the air-water interface, this project will activate and protect the enzymes, increasing their lifespan and reducing costs. By understanding mass transfer and enzyme activity in the interfacial immobilisation media, floating enzyme rafts can be developed for deployment over expansive areas, facilitating large-scale conversion of atmospheric CO2 into algae-derived fuels, feeds and chemicals.Read moreRead less
Combining new synthetic biology tools to boost crop CO2 capture and growth. A solution for improving crop yield is to enhance the carbon dioxide fixation properties of the enzyme Rubisco whose inefficient activity often limits plant growth. This project makes use of new synthetic biology capabilities to artificially evolve Rubisco in the laboratory and select for new versions with improved performance. These beneficial changes will be introduced into crop Rubisco using targeted gene editing appr ....Combining new synthetic biology tools to boost crop CO2 capture and growth. A solution for improving crop yield is to enhance the carbon dioxide fixation properties of the enzyme Rubisco whose inefficient activity often limits plant growth. This project makes use of new synthetic biology capabilities to artificially evolve Rubisco in the laboratory and select for new versions with improved performance. These beneficial changes will be introduced into crop Rubisco using targeted gene editing approaches and the improvements in photosynthesis, growth and yield evaluated. This information will aid complimentary biotechnological efforts seeking to supercharge photosynthesis and help deliver the second Green Revolution needed to meet the improvement required in future agriculture productivity and resource use.Read moreRead less
Development of electrophoretic cell sorters. The aim of this research is to develop the next generation of cell sorters to scale production of viable sperm and other cell types. Our approach is to understand the factors that control the migration of cells in an electric field so that we can design large-scale devices to purify cells using electrophoretic separation. The outcomes of this research enable large-scale production of viable sperm for human and animal-assisted conception, benefiting in ....Development of electrophoretic cell sorters. The aim of this research is to develop the next generation of cell sorters to scale production of viable sperm and other cell types. Our approach is to understand the factors that control the migration of cells in an electric field so that we can design large-scale devices to purify cells using electrophoretic separation. The outcomes of this research enable large-scale production of viable sperm for human and animal-assisted conception, benefiting infertile couples and the livestock breeding industry. Successful development of an efficient cell separation technology would add significant commercial value to the Australian biomanufacturing sector. Read moreRead less
High performance bioderived hybrid fillers for rubber composite. This project aims to address a significant problem in polymer composite synthesis by production and application of high performance bioderived hybrid silica fillers from renewable biomass feedstock. The project expects to generate new knowledge in the area of advanced manufacturing using interdisciplinary approaches in biorefining, filler and composite production and characterization. Expected outcomes of this project include a mo ....High performance bioderived hybrid fillers for rubber composite. This project aims to address a significant problem in polymer composite synthesis by production and application of high performance bioderived hybrid silica fillers from renewable biomass feedstock. The project expects to generate new knowledge in the area of advanced manufacturing using interdisciplinary approaches in biorefining, filler and composite production and characterization. Expected outcomes of this project include a more sustainable filler production process for producing novel bioderived silica fillers with properties superior to commercial silica fillers. The successful implementation of this project will lead to the development of a new advanced manufacturing industry, creating jobs in regional Australia. Read moreRead less
Industrial biotechnology for the manufacture of alkaloid pharmaceuticals. Drugs that treat opioid dependence and overdose are increasingly needed but their manufacture is difficult, inefficient and expensive. This project aims to develop enzymatic N-demethylation as a simpler and more sustainable approach to the manufacture and modification of opioid antagonists, alkaloids and other drug targets. It will increase our understanding of enzymatic N-demethylation and address engineering and biotechn ....Industrial biotechnology for the manufacture of alkaloid pharmaceuticals. Drugs that treat opioid dependence and overdose are increasingly needed but their manufacture is difficult, inefficient and expensive. This project aims to develop enzymatic N-demethylation as a simpler and more sustainable approach to the manufacture and modification of opioid antagonists, alkaloids and other drug targets. It will increase our understanding of enzymatic N-demethylation and address engineering and biotechnology challenges to improve yield and product isolation and concentration. Improvements in drug manufacturing processes will assist drug affected communities and industry will benefit from value adding, greater market share and flexibility, increased innovation and de-risked processes and new products. Read moreRead less
Light-driven biocatalytic cell factories. This project aims to develop single-cell algae optimised for high-efficiency green hydrogen production from cheap, sustainable resources - sunlight and water. The success of this project would be a game changer for industry by dramatically improving light to fuel conversion efficiencies. The expected high-efficiency cells would be a valuable resource for a wide range of other light-driven advanced bio-manufacture applications from high-value biopharmaceu ....Light-driven biocatalytic cell factories. This project aims to develop single-cell algae optimised for high-efficiency green hydrogen production from cheap, sustainable resources - sunlight and water. The success of this project would be a game changer for industry by dramatically improving light to fuel conversion efficiencies. The expected high-efficiency cells would be a valuable resource for a wide range of other light-driven advanced bio-manufacture applications from high-value biopharmaceuticals (e.g. <10 Hectare scale) through to renewable fuels (e.g. 10-1000 Hectare regional scale). The benefits would include advanced green chemical and biochemical manufacturing, diversified sources for green H2 production, regional development, industry growth, job security and exports.Read moreRead less